Measuring ATP in Axons with FRET

Shields, Lauren; Mendelsohn, Bryce A.; Nakamura, Ken

doi:10.1007/978-1-4939-6890-9_6

Cited by 3 publications

(1 citation statement)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ATP is one of the most abundant metabolites in a cell, ranging from 1 to 5 mM in mammalian cells (83). Intracellular distribution of ATP, detected by several types of genetic ATP biosensors (81), shows discrete ATP levels in organelles (84)(85)(86)(87). There is also a report showing elevated ATP in the cortical region (88).…”

Section: Heterogenous Purine Nucleotide Metabolism In Cell Membrane-pmentioning

confidence: 99%

Metabolic Compartmentalization at the Leading Edge of Metastatic Cancer Cells

et al. 2020

View full text Add to dashboard Cite

Despite advances in targeted therapeutics and understanding in molecular mechanisms, metastasis remains a substantial obstacle for cancer treatment. Acquired genetic mutations and transcriptional changes can promote the spread of primary tumor cells to distant tissues. Additionally, recent studies have uncovered that metabolic reprogramming of cancer cells is tightly associated with cancer metastasis. However, whether intracellular metabolism is spatially and temporally regulated for cancer cell migration and invasion is understudied. In this review, we highlight the emergence of a concept, termed "membraneless metabolic compartmentalization," as one of the critical mechanisms that determines the metastatic capacity of cancer cells. In particular, we focus on the compartmentalization of purine nucleotide metabolism (e.g., ATP and GTP) at the leading edge of migrating cancer cells through the uniquely phase-separated microdomains where dynamic exchange of nucleotide metabolic enzymes takes place. We will discuss how future insights may usher in a novel class of therapeutics specifically targeting the metabolic compartmentalization that drives tumor metastasis.

show abstract

Section: Heterogenous Purine Nucleotide Metabolism In Cell Membrane-pmentioning

confidence: 99%

Metabolic Compartmentalization at the Leading Edge of Metastatic Cancer Cells

et al. 2020

View full text Add to dashboard Cite

show abstract

Isoflurane inhibition of endocytosis is an anesthetic mechanism of action

Jung¹,

Zimin

Woods³

et al. 2022

Current Biology

View full text Add to dashboard Cite

Nanometer-Resolution Long-term Tracking of Single Cargos Reveals Dynein Motor Mechanisms

Peng

Zhang

Liu

et al. 2022

Preprint

View full text Add to dashboard Cite

Cytoplasmic dynein is essential for intracellular transport, but because of its complexity, we still do not fully understand how this 1.5 megadalton protein works. Here, we used novel optical probes that enable single-particle tracking (SPT) of individual cargos transported by dynein motors in live neurons over 900 μm. Analyses using the Fluctuation Theorem (FT) showed that the number of dynein molecules switches between 1-5 motors during the transport. Clearly resolved single-molecular steps revealed that the dwell times between individual steps were accurately described by an enzymatic cycle dominated by two equal and thermally-activated rate constants. Based on these data, we propose a new molecular model whereby each step requires the hydrolysis of 2 ATPs. The model is consistent with extensive structural, single-molecule and biochemical measurements.

show abstract

Measuring ATP in Axons with FRET

Cited by 3 publications

References 35 publications

Metabolic Compartmentalization at the Leading Edge of Metastatic Cancer Cells

Metabolic Compartmentalization at the Leading Edge of Metastatic Cancer Cells

Isoflurane inhibition of endocytosis is an anesthetic mechanism of action

Nanometer-Resolution Long-term Tracking of Single Cargos Reveals Dynein Motor Mechanisms

Contact Info

Product

Resources

About