Measuring and modeling of binary mixture effects of pharmaceuticals and nickel on cell viability/cytotoxicity in the human hepatoma derived cell line HepG2

Similarities and Differences in the Expression of Drug-Metabolizing Enzymes between Human Hepatic Cell Lines and Primary Human Hepatocytes

“…In addition to primary human hepatocytes, hepatoma cell lines and immortalized or transfected nonhepatoma hepatic cell lines have been used for pharmacological and toxicological studies (Dykens et al, 2008;Rudzok et al, 2010). However, their limitations with respect to FIG.…”

“…On the other hand, HepG2 hepatoma cells are relatively easy to maintain in culture and are widely used for toxicity studies. Despite the low activities of certain drug-metabolizing enzymes, such as CYP3A4, CYP2A6, CYP2C9, and CYP2C19, in comparison with primary human hepatocytes (Westerink and Schoonen, 2007), the HepG2 cell line has been considered a valuable model and is used for risk assessment of toxicants and toxins because it retains several liver functions (Dykens et al, 2008;Rudzok et al, 2010). In addition, other human hepatoma cell lines, such as Huh7, SK-Hep-1, Hep3B, and HepaRG have also been used in drug metabolism and toxicity studies (Henzel et al, 2004;Knasmüller et al, 2004;Shiizaki et al, 2005;Aninat et al, 2006;Suzuki et al, 2008;Chao et al, 2009;Wee et al, 2009).…”

Investigation of the state of the science on combined actions of chemicals in food through dissimilar modes of action and proposal for science‐based approach for performing related cumulative risk assessment

“…• The MIXTOX model, developed in EU project ENV4-CT97-0507, was evaluated using binary mixture studies of nickel, irgasan (an antibiotic) and diclofenac (an antiinflammatory) (Rudzok et al 2010). The model incorporates the IA and DA models, 54…”

A Strategy Using Pluripotent Stem Cell-Derived Hepatocytes for Stem Cell-Based Therapies

“…Therefore, the cultured hepatocyte can be used to predict how drugs are metabolized and to what extent they may be toxic. These tests include use of primary rodent hepatocytes and human immortalised hepatocyte cell lines, such as HepG2 (Rudzok et al, 2010). However, the rodent hepatocytes have species-specific differences in metabolizing function from human (Lake, 2009) and immortalised hepatocytes have poor hepatic function and sometimes have an abnormal karyotype (Wong et al, 2000).…”