Understanding the drivers of pro-environmental behaviour (PEB) is essential, as environmental degradation stemming from human actions is a profound challenge for humanity. Research suggests that in addition to connection to nature and environmental attitudes, interest in nature, especially when aroused in youth, can be a significant motivational factor driving people’s engagement in PEB. However, the relationship between interest in nature and other determinants of PEB, as well as their collective influence on PEB on adolescents, is still greatly understudied. We aim to bridge this gap by incorporating interest in nature into a model explaining PEB. We used data from a cross-sectional study with German lower secondary school students (n = 1092, age 10-15 years), applying four self- report scales: 1) interest in nature (SIN), 2) connection to nature (DCN), 3) environmental attitudes (2-MEV), and 4) general ecological behaviour (GEB). Our findings demonstrate that interest in nature not only positively correlates with other determinants of PEB but also significantly influences PEB itself. This suggests that cultivating interest in nature among adolescents can potentially lay the groundwork for their development of PEB. Therefore, interest in nature should be considered in future research studies both exploring PEB and evaluating the effectiveness of ESD programs.