MEASUREMENTS OF PHYTOPLANKTON‐PROTEIN CONTENT WITH THE HEATED BIURET‐FOL1N ASSAY<sup>1</sup>,<sup>2</sup>

Dorsey, Thomas E.; McDonald, Paul; Roels, Oswald A.

doi:10.1111/j.1529-8817.1978.tb02443.x

Cited by 39 publications

(12 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Total carbohydrate was determined by using the method of Kochert (1978) as modified by Ben Amotz et al (1985). Total protein content was determined using a modified Lowry et al (1951) method based on Dorsey et al (1978).…”

Section: Methodsmentioning

confidence: 99%

Comparison of continuous and day time only mixing on Tetraselmis suecica (Chlorophyta) in outdoor raceway ponds

et al. 2014

View full text Add to dashboard Cite

The mixing of microalgae bioreactors is important as it circulates cells in and out of the light zone and reduces boundary layers, benefiting photosynthesis and nutrient uptake. However, mixing is also a major economical factor, impacting on power consumption and operating costs, and it remains a barrier for those locations where no electrical supply is available. Combining solar panels with algae ponds can help to alleviate the power cost burden, but if solar panels are the sole source of generated electricity for a regional raceway pond system, the paddle wheels will only operate during day time. This study investigated the growth of halophilic green alga Tetraselmis suecica, in outdoor paddle wheel-driven open ponds under three different mixing regimes. The first experimental condition was a continuously mixed regime, while in the second and third conditions, the paddle wheels were stopped overnight and restarted 1 h after sunrise or 1 h before sunrise, respectively. Growth rate, biomass productivity and cell weight showed no statistically significant difference during the period when the culture was growing under optimal environmental conditions. Chlorophyll fluorescence measurements of photosystem II maximum quantum yield in the light (F v ′/F m ′) over a 24-h period found no significant differences between cultures, further suggesting that under favourable conditions ceasing mixing overnight may have no undesirable impact on Tetraselmis health or productivity. These findings imply a potential 37 % power saving and a 33 % reduction in energy-associated costs. During a heat stress event, Tetraselmis was negatively impacted in all ponds to the same degree; however, the continuously mixed pond recovered faster than ponds that were not mixed overnight.

show abstract

Section: Methodsmentioning

confidence: 99%

Comparison of continuous and day time only mixing on Tetraselmis suecica (Chlorophyta) in outdoor raceway ponds

et al. 2014

View full text Add to dashboard Cite

show abstract

“…A time-course experiment was conducted during which triplicate samples from each culture were taken morning and evening (just before lights-on and lights-off) for analysis of chlorophyll, nitrate, protein, and carbohydrate. Chlorophyll and nitrate were analyzed as described for tank experiments, protein was analyzed by the Biuret-Folin method of Dorsey et al (1978) using bovine serum albumin (BSA) as standard, and carbohydrate was measured as glucose equivalents by the phenolsulphuric acid method of Dubois et al (1956). Samples were taken before and after depletion of nitrate from th.e culture medium.…”

Section: Methodsmentioning

confidence: 99%

Changes in buoyancy and chemical composition during growth of a coastal marine diatom:ecological and biogeochemical consequences

Richardson¹,

Cullen²

1995

Mar. Ecol. Prog. Ser.

View full text Add to dashboard Cite

Growth and sinklng of the coastal manne diatom Thalassiosira weissflogii was studled dunng experiments in a 2 1 m tall water column Under nutnent-replete conditions, T weissflogii grew rapidly in the upper layer of the tank and cells exhibited nearly neutral buoyancy Cells sank however, after depletion of ambient nitrate The growth and sinking of cells were well described by a simple model with nitrate-dependent growth and sinking terms The best fit was obtained by describing the sinking response with a slgmoid functlon ~v h e r e the time scale for increased sinking was 25 h after nitrate depletion Smaller-scale batch culture experiments showed changes that were consistent wlth increased slnking upon depletion of nitrate carbohydrate per m1 of culture increased 2-to 5-fold over 3 5 d , while protein per m1 did not change signif~cantly After re-introduction of nitrate into the medium carbohydrate protein ratios reverted to their onginal values Calculations show that intracellular density and cell sinking rate should change substantially due to fluctuations in carbohydrate and that increased ballast of carbohydrate was sufficient to cause the slnking observed in the tank Changes in the buoyancy of small (15 pm) diatoms such as T weissflogii In response to changes in nutr~ent status have important ecological and biogeochemical implications Nutrient-dependent changes in sinking rates can result in ~ncreased residence time of cells in the mixed layer of the ocean, and In enhanced transport of deep nutnents to the euphotic zone uncoupled from inputs of inorganic carbon

show abstract

“…Protein concentration was determined according to Strain et al (2006) based on Dorsey et al (1978) modified from Lowry et al (1951). Briefly, 1 mg freeze-dried phytoplankton was hydrolyzed in 10 ml Biuret reagent (diluted 1:1 with distilled water) at 100°C for 1 h and then mixed with 0.5 ml Folin-phenol reagent.…”

Section: Phytoplankton and Biochemical Compositionmentioning

confidence: 99%

Digestive enzymes and in-vitro digestibility of different species of phytoplankton for culture of the freshwater pearl mussel, Hyriopsis (Hyriopsis) bialatus

et al. 2007

View full text Add to dashboard Cite

Hyriopsis (Hyriopsis) bialatus has been cultured during the mussel life cycle from glochidia to the adult stage with a low total survival of 6% up to 130-day-old juveniles. The main digestive enzymes (amylase and proteinases) were not detectable in one-day-old juveniles, and increased during development. The stomach, including digestive glands, was the major digestive organ for both carbohydrate and protein. The optimum conditions for amylase activity were 40°C and pH 7; for acidic proteinases they were 60°C and pH 5. Two main alkaline proteinases were found in the intestine, with optimum conditions of 30°C and pH 8 and 60°C and pH 8. To improve mussel survival by finding suitable phytoplankton species and age as food for juveniles and adults, an in-vitro digestibility test was performed on ten algal species three and seven days old using amylase and proteinases in crude enzyme extracts from different mussel life stages. Among the phytoplankton selected, the three most efficiently digested by juveniles were sevenday-old Chlorella sp.2, seven-day-old Chlorococcum sp. and seven-day-old Kirchneriella incurvata, in the ratio 1:1:3 for 30-day-old juveniles and 3:1:1 for 130-day-old juveniles. For the adult mussel, three-day-old Chlorella sp.2, seven-day-old Coccomyxa sp., and seven-day-old Monoraphidium sp., in the ratio 3:1:1, were the most digestible phytoplankton. Levels of in-vitro digestibility were related to the quality (not the concentrations) of carbohydrate and protein in the phytoplankton mixtures, and protein digestibility seemed to be the key factor determining food quality for the mussel.

show abstract

MEASUREMENTS OF PHYTOPLANKTON‐PROTEIN CONTENT WITH THE HEATED BIURET‐FOL1N ASSAY¹,²

Cited by 39 publications

References 10 publications

Comparison of continuous and day time only mixing on Tetraselmis suecica (Chlorophyta) in outdoor raceway ponds

Comparison of continuous and day time only mixing on Tetraselmis suecica (Chlorophyta) in outdoor raceway ponds

Changes in buoyancy and chemical composition during growth of a coastal marine diatom:ecological and biogeochemical consequences

Digestive enzymes and in-vitro digestibility of different species of phytoplankton for culture of the freshwater pearl mussel, Hyriopsis (Hyriopsis) bialatus

Contact Info

Product

Resources

About

MEASUREMENTS OF PHYTOPLANKTON‐PROTEIN CONTENT WITH THE HEATED BIURET‐FOL1N ASSAY1,2

Cited by 39 publications

References 10 publications

Comparison of continuous and day time only mixing on Tetraselmis suecica (Chlorophyta) in outdoor raceway ponds

Comparison of continuous and day time only mixing on Tetraselmis suecica (Chlorophyta) in outdoor raceway ponds

Changes in buoyancy and chemical composition during growth of a coastal marine diatom:ecological and biogeochemical consequences

Digestive enzymes and in-vitro digestibility of different species of phytoplankton for culture of the freshwater pearl mussel, Hyriopsis (Hyriopsis) bialatus

Contact Info

Product

Resources

About

MEASUREMENTS OF PHYTOPLANKTON‐PROTEIN CONTENT WITH THE HEATED BIURET‐FOL1N ASSAY¹,²