Measurements of nitric oxide in biological materials using a porphyrinic microsensor

Maliński, Tadeusz; Taha, Ziad; Grunfeld, Saul; Burewicz, Andrzej; Tomboulian, Paul; Kiechle, Frederick L.

doi:10.1016/0003-2670(93)85075-u

Cited by 118 publications

(47 citation statements)

References 16 publications

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“…The endothelium contributes to the control of vascular smooth muscle tone by the release of nitric oxide (NO), which accounts for the biological activity of the endothelium-derived relaxing factor (1)(2)(3)(4). NO causes vasodilatation and platelet inhibition and thereby prevents vasoconstriction and thrombus formation (5).…”

Section: Introductionmentioning

confidence: 99%

Effect of age on kinetics of nitric oxide release in rat aorta and pulmonary artery.

Tschudi¹,

Barton²,

Bersinger³

et al. 1996

J. Clin. Invest.

251

160

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Aging is an important determinant of vascular disease. Endothelium-derived nitric oxide (NO) is protective as a vasodilator and inhibitor of platelet function. This study was designed to directly measure effects of prolonged aging on endothelial NO release in isolated blood vessels and to delineate differences between the systemic and pulmonary circulation.Aortas and pulmonary arteries from 5-6-mo-old (young), 18-19-mo-old (middle-aged), and 32-33-mo-old (old) normotensive female rats were used. Blood pressure and plasma estradiol-17 ␤ (E 2 ) remained unchanged. In isolated blood vessels, NO release was induced by the receptor-independent agonist calcium ionophore A23187 (10 mol/liter) and measured in situ on the endothelial surface of vessels using a porphyrinic microsensor. In vessels suspended in organ chambers isometric tension was recorded. In the aorta, the initial rate of NO release and peak NO concentration were reduced in middle-aged and old rats ( P Ͻ 0.0006 vs. young rats, n ϭ 6). Furthermore, endothelium-dependent relaxations to calcium ionophore and acetylcholine (both 10 Ϫ 10 -10 Ϫ 5 mol/liter) were also reduced in aortas from old as compared with young rats ( n ϭ 6, P Ͻ 0.05). The initial rate of NO release and peak NO concentration significantly correlated with maximal relaxation to calcium ionophore A23187 (correlation coefficients r ϭ 0.916, P Ͻ 0.0018 and r ϭ 0.961, P Ͻ 0.0001, respectively, n ϭ 7). In pulmonary arteries, however, the initial rate of NO release as well as peak NO concentration did not decrease with age ( n ϭ 6 for each age group, NS). In both blood vessels, the NO release was unaffected by superoxide dismutase in all age groups ( n ϭ 6, NS).Thus, aging specifically reduces initial rate and peak concentrations of endothelial NO release from aorta but not pulmonary artery indicating reduced NO production. As arterial pressure did not change with aging, the chronic exposure of the aorta to higher pressure and/or pulsatility than in the pulmonary artery may be the cause. This appears important as NO plays a protective role by preventing vasoconstriction, thrombosis and atherosclerosis.

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Section: Introductionmentioning

confidence: 99%

Effect of age on kinetics of nitric oxide release in rat aorta and pulmonary artery.

Tschudi¹,

Barton²,

Bersinger³

et al. 1996

J. Clin. Invest.

251

160

View full text Add to dashboard Cite

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“…Devido às suas propriedades e características, este tipo de eletrodo tem se tornado uma ferramenta decisiva nas análises e determinações de: (a) contaminantes ambientais 1,2 , (b) moléculas bioquímicas, incluindo medicamentos [3][4][5][6] , (c) metais pesados ou substâncias tóxicas em alimentos [7][8][9] , etc. Além disto, vários processos eletródicos têm sido estudados sob uma nova ótica, originada da utilização dos UME.…”

Section: Introductionunclassified

Ultramicroeletrodos. Parte II: construção e aplicações

et al. 1998

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Recebido em 16/10/96; aceito em 14/1/97 ULTRAMICROELECTRODES. PART II: CONSTRUCTION AND APPLICATIONS. This work describes the techniques of construction and several applications of ultramicroelectrodes in electrochemistry and electroanalytical chemistry. Disc shaped UME are produced by embedding metal wires on insulating materials such as glass or epoxy resin. In the field of electrochemistry, UME have been applied in studies of the hydrogen evolution reaction and the electrocrystallization of metals. The negligible values of sensibility for ohmic drop and the enhanced mass transport rate by spherical diffusion are the main advantages of UME in these applications. New important conclusions regarding the phenomena under study were drawn from the experimental results. The applications in electroanalytical chemistry involved the determination of contaminants such as heavy metals and nitrites in natural waters and food products. The use of UME requires little sample manipulation and, in general, no need for oxygen removal or the addition of supporting electrolytes.

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“…Certain electroactive neurotransmitters (eg Nitric Oxide, dopamine) are amenable to detection via electrochemical analysis. Electrochemical analysis of neurotransmitters is very attractive due to high sensitivity, rapidity and the ability to perform distributed measurements [1,2,3].…”

Section: Introductionmentioning

confidence: 99%

16-channel wide-range VLSI potentiostat array

Stanaćević

Murari

Cauwenberghs

et al.

IEEE International Workshop on Biomedical Circuits and Systems, 2004.

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A 16-Channel current-measuring VLSI sensor array system for electrochemical detection of electroactive neurotransmiters like dopamine and nitric-oxide is presented. The designed ADC architecture is first-order single bit delta-sigma modulator with programmable oversampling ratio. The modulation of feedback duty-cycle in modulator design enables choice of current range in accordance to magnitude and time constant of signal leading to current range that spans over six orders of magnitude and sensitivity up to picoamperes at hundred millisecond conversion time with power consumption of 300 µW. A 3mm x 3mm chip in 0.5 µm CMOS technology is used for real-time multi-channel acquisition of neurotransmitter concentration.

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Measurements of nitric oxide in biological materials using a porphyrinic microsensor

Cited by 118 publications

References 16 publications

Effect of age on kinetics of nitric oxide release in rat aorta and pulmonary artery.

Effect of age on kinetics of nitric oxide release in rat aorta and pulmonary artery.

Ultramicroeletrodos. Parte II: construção e aplicações

16-channel wide-range VLSI potentiostat array

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