Measurements of Microbial Biomass C and N in Paddy Soils by the Fumigation-Extraction Method

An Empirical Model of Soil Chemical Properties that Regulate Methane Production in Japanese Rice Paddy Soils

“…Mean mineralized N averaged 123.62 units with a CV of 59.9%. The mean ratio of total decomposed C to mineralized N was 5.62 with a low CV of 19.1%, which is comparable to Redfield ratio (C/N = 5.67) and soil microbial biomass C and N ratio in rice paddies reported by Shibahara and Inubushi (1995) Compared to the mean total C/N (11.64, Table 1), the ratio of decomposed C to mineralized N in rice soil was lower than that of the bulk soil organic matter pool, which indicated that the easily decomposed organic matter was from soil microbial biomass and humified organic matter in the soils. Also, the relationship between total decomposed C and mineralized N was significant (Fig.…”

Spatial Variability of Nitrous Oxide Emissions and Their Soil‐Related Determining Factors in an Agricultural Field

“…The C status (an indicator of energy sources available to microorganisms) and contents of exchangeable cations and available P were also appreciable. Biological properties further showed that the level of microbial biomass N and C and their ratio to total N and C were relatively high, possibly because the biomass community still reflected the original paddy‐field condition, for which such ratios are generally high (Shibahara and Inubushi, 1995). The CV values suggested that the soil properties measured had less variability than the N 2 O fluxes.…”

Role of microbial biomass in biogeochemical processes in paddy soil environments

“…The mixture was further fumigated with chloroform vapor for 24 h in a desiccator. After this initial attempt, the chloroform fumigationextraction method has been widely used for microbial biomass measurements in flooded paddy soils (Shibahara and Inubushi 1995Inubushi et al 1999Inubushi et al , 2001Inubushi et al , 2002Acquaye et al 2000;Cheng et al 2000;Hoque et al 2001;Acquaye and Inubushi 2004).…”