Measurements of leukotrienes in human plasma by solid phase extraction and high performance liquid chromatography

Henden, T.; Strand, Harald; Børde, E.; Semb, Anne Grete; Larsen, Terje S.

doi:10.1016/0952-3278(93)90209-f

Cited by 7 publications

(3 citation statements)

References 8 publications

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“…Current analytical techniques for the separation of eicosanoids in standards as well as biological matrices include high-performance liquid chromatography with both UV (HPLC-UV) [22][23][24][25] and fluorescence (HPLC-FL) [26][27][28][29] detection, capillary electrophoresis with UV detection (CE-UV) [30] and gas chromatography-mass spectrometry (GC-MS) [31][32][33]. The above techniques have significant disadvantages that limit their effectiveness in bioanalytical applications.…”

Section: Analysis Of Eicosanoidsmentioning

confidence: 99%

Development and validation of a high-performance liquid chromatography–electrospray mass spectrometry method for the simultaneous determination of 23 eicosanoids

Blewett

Varma

Gilles

et al. 2008

Journal of Pharmaceutical and Biomedical Analysis

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Section: Analysis Of Eicosanoidsmentioning

confidence: 99%

Development and validation of a high-performance liquid chromatography–electrospray mass spectrometry method for the simultaneous determination of 23 eicosanoids

Blewett

Varma

Gilles

et al. 2008

Journal of Pharmaceutical and Biomedical Analysis

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“…Current analytical techniques for the separation of biological matrices include high-performance liquid chromatography with both UV (LC-UV) [7,8,9,10] and fluorescence (LC-FL) [11,12,13,14] detection, capillary electrophoresis with UV detection (CE-UV) [15] and gas chromatography–mass spectrometry (GC–MS) [16,17,18]. The above techniques, which are popular methods for analyzing biosamples, have disadvantages that may limit their effectiveness in bioanalytical applications.…”

Section: Introductionmentioning

confidence: 99%

A LC-MS-Based Method for Quantification of Biomarkers from Serum of Allergic Rats

Bai

Gao²,

Lu³

et al. 2010

Molecules

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Allergies are highly complex disorders with clinical manifestations ranging from mild oral, gastrointestinal, recurrent wheezing, and cutaneous symptoms to lifethreatening systemic conditions. The levels of arachidonic acid, eicosanoids, histamine, organic acids and valine are considered to have a variety of physiological functions in connection with allergies. In this research, we have developed a RP-LC/MS method to separate and quantitate six different potential endogenous biomarkers, including leukotrieneB 4 (LTB 4 ), prostaglandinD 2 (PGD 2 ), arachidonic acid (AA), histamine (HI), lactic acid (LA) and valine (VAL), from serum of rats with ovalbumin (OVA)-induced allergy and normal rats, and the discrepancies between the model group and the control group were compared. The separation was performed on a Prevail C 18 column (250 mm × 4.6 mm, 5 μm) with a gradient elution of acetonitrile with 0.1% formic acid (v/v) and 10 mM ammonium formate (adjusted to pH 4.0 with formic acid) at a flow rate of 0.5 mL min −1 The method was validated and shown to be sensitive, accurate (recovery values 76.16-92.57%) and precise (RSD < 10% for all compounds) with a linear range over several orders of magnitude. The method was successfully applied to rat serum and shown to be indicative of the endogenous levels of biomarkers within the rat body. The OPEN ACCESSMolecules 2010, 15 3357 analysis of the biomarkers can provide insight into the allergic mechanisms associated with related diseases.

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“…Various publications have described the determination of leukotrienes in the blood plasma (Shindo et al, 1997;Henden et al, 1993) urine (Armstrong et al, 2009;Misso et al, 2004;Higashi et al, 2004), EBC (Syslova et al, 2011;Brussino et al, 2010;Biko et al, 2010) and saliva (McKinney et al, 2000;Tufvesson et al, 2010 Immunochemical methods offer simple, rapid, robust yet sensitive, and easily automated methods for routine analyses in clinical laboratories. Immunoassays are based on highly specific binding between an antigen and an antibody.…”

Section: Methods For Determination Of Cysteinyl Leukotrienesmentioning

confidence: 99%

Determination of Biomarkers in Exhaled Breath Condensate: A Perspective Way in Bronchial Asthma Diagnostics

Syslová¹,

Kačer²,

Kuzma³

et al. 2012

Bronchial Asthma - Emerging Therapeutic Strategies

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Measurements of leukotrienes in human plasma by solid phase extraction and high performance liquid chromatography

Cited by 7 publications

References 8 publications

Development and validation of a high-performance liquid chromatography–electrospray mass spectrometry method for the simultaneous determination of 23 eicosanoids

Development and validation of a high-performance liquid chromatography–electrospray mass spectrometry method for the simultaneous determination of 23 eicosanoids

A LC-MS-Based Method for Quantification of Biomarkers from Serum of Allergic Rats

Determination of Biomarkers in Exhaled Breath Condensate: A Perspective Way in Bronchial Asthma Diagnostics

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