Measurements of forces involved in vesicle adhesion using freeze-fracture electron microscopy

Bailey, Stuart; Chiruvolu, S.; Israelachvili, Jacob N.; Zasadzinski, Joseph A.

doi:10.1021/la00097a024

Cited by 52 publications

(60 citation statements)

References 2 publications

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“…There were essentially no vesicles with three layers or more. The vesicles in 1% NaBr sample also had a greater tendency to adhere both to each other and the carbon-coated electron microscope grid (23) and flatten, consistent with the enhanced attraction between the vesicle bilayers (41).…”

Section: ϫ2supporting

confidence: 53%

“…There were essentially no vesicles with three layers or more. The vesicles in the 1% NaBr sample also had a greater tendency to adhere both to each other and the polymer-coated electron microscope grid (23) and flatten, consistent with the enhanced attraction between the vesicle bilayers (41). Some of the vesicles are clustered and appear polygonal; the vesicles can come into closer proximity because of the screening of the residual electrostatic forces, indicative of the net attractive forces between the bilayers.…”

Section: ϫ2mentioning

confidence: 54%

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The origins of stability of spontaneous vesicles

Jung¹

2001

Proceedings of the National Academy of Sciences

107

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Equilibrium unilamellar vesicles are stabilized by one of two distinct mechanisms depending on the value of the bending constant. Helfrich undulations ensure that the interbilayer potential is always repulsive when the bending constant, K, is of order k BT. When K Ͼ Ͼ k BT, unilamellar vesicles are stabilized by the spontaneous curvature that picks out a particular vesicle radius; other radii are disfavored energetically. We present measurements of the bilayer elastic constant and the spontaneous curvature, R o, for three different systems of equilibrium vesicles by an analysis of the vesicle size distribution determined by cryo-transmission electron microscopy and smallangle neutron scattering. For cetyltrimethylammonium bromide (CTAB)͞sodium octyl sulfonate catanionic vesicles, K ‫؍‬ .7 k BT, suggesting that the unilamellar vesicles are stabilized by Helfrich-undulation repulsions. However, for CTAB and sodium perfluorooctanoate (FC7) vesicles, K ‫؍‬ 6 kBT, suggesting stabilization by the energetic costs of deviations from the spontaneous curvature. Adding electrolyte to the sodium perfluorooctanoate͞CTAB vesicles leads to vesicles with two bilayers; the attractive interactions between the bilayers can overcome the cost of small deviations from the spontaneous curvature to form two-layer vesicles, but larger deviations to form three and more layer vesicles are prohibited. Vesicles with a discrete numbers of bilayers at equilibrium are possible only for bilayers with a large bending modulus coupled with a spontaneous curvature.

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Section: ϫ2supporting

confidence: 53%

Section: ϫ2mentioning

confidence: 54%

The origins of stability of spontaneous vesicles

Jung¹

2001

Proceedings of the National Academy of Sciences

107

View full text Add to dashboard Cite

show abstract

“…There were essentially no vesicles with three layers or more. The vesicles in 1% NaBr sample also had a greater tendency to adhere to each other and the carbon coated electron microscope [93]. The distribution between one layer and two-layer vesicles can be derived using the mass action model for vesicles with a spontaneous bilayer curvature.…”

Section: Close-packed Vesicle Dispersions Andmentioning

confidence: 99%

Phase Behavior and Microstructure in Aqueous Mixtures of Cationic and Anionic Surfactants

Kaler¹,

Herrington²,

Iampietro³

et al. 2004

Surfactant Science

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][ [Page No. 290] {Books}4380-Abe/4380-Abe-009.3d 4380-Abe SYNOPSISMixtures of anionic and cationic surfactants in water display interesting phase behavior and a range of microstructures, including small micelles, rod-like micelles, lamellar phases and vesicles. This chapter reviews the properties of these mixtures with a focus on the experimental and theoretical aspects of vesicle formation and stability.

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“…Vesicles are often used as model systems to study solute permeability through bilayers (Lossen, 1972;Selser et al, 1976;Brunner et al, 1980;Carruthers and Melchior, 1983;Castaing et al, 1992), bilayer elasticity (Servuss et al, 1976;Schneider, Jenkins and Webb, 1984;Milon et al, 1986;Sun et al, 1986;Li et al, 1986;Haines et al, 1987;Miyamoto et al, 1988;Duwe and Sackmann, 1990;Rutkowski et al, 1991), and interactions between bilayer membranes (Evans and Needham, 1987;Servuss and Helfrich, 1989;Bailey et al, 1990). In many of these experiments, concentration differences exist between the inside and outside of the vesicle membrane, leading to an osmotic swelling or shrinkage of the vesicles.…”

Section: Typical Experiments To Measure Permeability In Biologically mentioning

confidence: 99%

Membrane elasticity effects on permeability measurements in vesicles

Chiruvolu

Zasadzinski

1993

AIChE Journal

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More than a quarter of a century ago, Bangham and coworkers (1967) showed that phospholipids dispersed in water formed closed, multibilayer aggregates called vesicles or liposomes, which are capable of separating an internal compartment from the bulk solution. These phospholipid and synthetic surfactant bilayers have osmotic and elastic properties similar to those of biological cell membranes and have been widely used as cell models (Papahadjopoulos and Miller, 1967;Huang, 1969;Evans and Skalak, 1980;Evans and Needham, 1987). Physico-mechanical properties, such as membrane area elasticity, membrane bending rigidity, critical tension for bursting (lysis), and membrane water and solute permeability, are important parameters in many cellular processes and functions like nutrient transport, cell-waste disposal, hemolysis, cell membrane adhesion and fusion and other immunological processes (

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Measurements of forces involved in vesicle adhesion using freeze-fracture electron microscopy

Cited by 52 publications

References 2 publications

The origins of stability of spontaneous vesicles

The origins of stability of spontaneous vesicles

Phase Behavior and Microstructure in Aqueous Mixtures of Cationic and Anionic Surfactants

Membrane elasticity effects on permeability measurements in vesicles

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