Measurements of cells in culture by scanning acoustic microscopy

Abstract

SUMMARY A novel method allowing the determination of thickness, attenuation and impedance of both living and fixed cells in culture using a single image recorded with a scanning acoustic microscope is described. The method is based on the recording of the image data locating the surface of the cell far below the geometric focus. In this way only the normal incident acoustic wave is used for image formation. Higher values of impedance and attenuation coefficients were found in the cell periphery than in the central part of the cell. This phenomenon is suggested to be due to the different organization of cytoskeletal elements.

Is expression of intracellular citrullinated proteins in synovial tissue specific for rheumatoid arthritis? Comment on the article by Baeten et al

“…SAM has been successfully used for many years for nondestructive investigation of elastic properties in a variety of applications in industry as well as in biology and medicine. Different hard and soft tissues have been investigated, such as bone,17–20 teeth,21, 22 or living cells 23–26…”

Numerical analysis of acoustic impedance microscope utilizing acoustic lens transducer to examine cultured cells

“…Several researchers have used SAM for cell observation [1][2][3][4][5]7]. Vector contrast SAM was developed for imaging living stem cells.…”

Mechanical property quantification of endothelial cells using scanning acoustic microscopy

“…Previous studies on cells have dealt with cell thickness measurement and morphological characterization [8][9][10][11][12]. The speed of sound in the cells has been measured using interference ring [11] and phase contrast imaging [13].…”