Measurements and analyses of electrophoretic mobilities of RAW 117 lymphosarcoma cells and their variant cells

Makino, Kimiko; Taki, Takao; Ogura, Misa; Handa, Shizuo; Nakajima, Motowo; Kondo, Tamotsu; Ohshima, Hiroyuki

doi:10.1016/0301-4622(93)80051-j

Cited by 38 publications

(19 citation statements)

References 7 publications

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“…Bubenikowa et al reported that EPM of mouse primary myeloma cells is −1.2×10 −4 cm 2 V -1 s -1 (ionic strength 0.14) [25]. Cell EPM is hardly affected by ionic strength of buffer solution when the ionic strength is over 0.05 [26,27]. Hence, EPMs obtained in this study are comparable and agree relatively well with previously reported data.…”

Section: Resultssupporting

confidence: 91%

Electrophoretic Mobility of Mouse Leukocytes Evaluated Using Microcapillary Electrophoresis Chips

Akagı

Hamada

Ichikı

2010

Trans. Mat. Res. Soc. Japan

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The electrophoretic mobilities (EPMs) of various leukocytes of mouse origin were measured using microcapillary electrophoresis (µCE) chips. The average EPMs of acute lymphoblastic leukemia (L1210), myeloma (PAI), myeloid leukemia (M1), and myelomonocytic leukemic (WEHI3) cell lines were −0.83×10 −4 , −1.0×10 −4 , −1.5×10 −4 , and −1.2×10 −4 cm 2 V -1 s -1 , respectively. The average EPMs of mouse primary blood leukocytes, bone marrow cells, and splenic cells were −2.0×10 −4 , −1.6×10 −4 , and −1.3×10 −4 cm 2 V -1 s -1 , respectively. EPM was different for each type of leukocyte. These quantitative data concerning cell surface charge are useful fundamental knowledge for understanding the interactions between cells and biomaterials, and for designing biomaterials.

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Section: Resultssupporting

confidence: 91%

Electrophoretic Mobility of Mouse Leukocytes Evaluated Using Microcapillary Electrophoresis Chips

Akagı

Hamada

Ichikı

2010

Trans. Mat. Res. Soc. Japan

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“…The mobility was measured as a function of the ionic strength of the dispersant media [26][27][28]. Different values of zN and 1/λ were obtained for the RAW-117P and RAW-117-H10 cell surfaces, as shown in figure 18.…”

Section: Differences In Surface Properties Of Malignant Lymphosarcomamentioning

confidence: 99%

Soft particle analysis of electrokinetics of biological cells and their model systems

Makino

Ohshima

2011

Science and Technology of Advanced Materials

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In this article, we review the applications of a novel theory (Ohshima 2009 Sci. Technol. Adv. Mater. 10 063001) to the analysis of electrokinetic data for various soft particles, that is, particles covered with an ion-permeable surface layer of polyelectrolytes. Soft particles discussed in this review include various biological cells and hydrogel-coated particles as a model of biological cells. Cellular transformations increase the concentration of sialic acid of glycoproteins and are associated with blocked biosynthesis of glycolipids and aberrant expression of the developmentally programmed biosynthetic pathway. The change in shape or biological function of cells may affect their surface properties and can be detected by electrokinetic measurements. The experimental results were analyzed with Ohshima's electrokinetic formula for soft particles and soft surfaces. As a model system, hydrogel surfaces that mimic biological surfaces were also prepared and their surface properties were studied.

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“…-U as r ??) and the deviation dm i (r) of the electrochemical potential dm i (r) of the i-th ionic species due to the applied field E. These equations are given by [5] Zr Â r Â r Â u gr Â u N i1 Further, symmetry considerations permit us to write [5,14] u r À 2 r hrEcosy;…”

Section: Fundamental Electrokinetic Equationsmentioning

confidence: 99%

“…where y r ec 0 r kT (14) is the scaled equilibrium potential outside the particle core, k is the Boltzmann's constant, and T is the absolute temperature.…”

Section: Fundamental Electrokinetic Equationsmentioning

confidence: 99%

“…This theory unites the electrophoresis theories of hard particles [1][2][3][4] and of polyelectrolytes [12], since a soft particle tends to a hard particle in the absence of the polymer layer and to a polyelectrolyte in the absence of the particle core. A number of electrophoresis measurements have been carried out on various types of soft particles [13][14][15][16][17][18][19][20][21][22][23][24][25][26], which include hydrogel coated latex particles [13][14][15][16] and biological cells [17][18][19][20][21][22][23][24][25], showing that the electrophoretic behaviors of these particles can be explained well by the electrophoresis theory of soft particles.…”

Section: Introductionmentioning

confidence: 99%

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Electrophoretic mobility of a charged spherical colloidal particle covered with an uncharged polymer layer

Ohshima

2002

Electrophoresis

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A general expression is derived for the electrophoretic mobility of a spherical charged colloidal particle covered with an uncharged polymer layer in an electrolyte solution in an applied electric field for the case where the particle zeta potential is low. It is assumed that electrolyte ions as well as water molecules can penetrate the polymer layer. Approximate analytic expressions for the electrophoretic mobility of particles carrying low zeta potentials are derived for the two extreme cases in which the particle radius is very large or very small.

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Measurements and analyses of electrophoretic mobilities of RAW 117 lymphosarcoma cells and their variant cells

Cited by 38 publications

References 7 publications

Electrophoretic Mobility of Mouse Leukocytes Evaluated Using Microcapillary Electrophoresis Chips

Electrophoretic Mobility of Mouse Leukocytes Evaluated Using Microcapillary Electrophoresis Chips

Soft particle analysis of electrokinetics of biological cells and their model systems

Electrophoretic mobility of a charged spherical colloidal particle covered with an uncharged polymer layer

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