Measurement Technologies for Upstream and Downstream Bioprocessing

Mandenius, Carl‐Fredrik

doi:10.3390/pr9010143

Cited by 2 publications

(2 citation statements)

References 15 publications

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“…[36,37] In this context, plasmonic nanostructures can be used in bioprocess engineering for biotherapeutics or vaccine production monitoring and bacteria and contaminant detection. [38,39] For example, a fiber optical nanoplasmonic sensor was designed for antibody immunoglobulin (IgG) monitoring in bioproduction, which enabled real-time observation and limited interference from unspecific proteins. [40] In previous work, we have designed, synthesized, and tested ceramic open cell foams based on zirconia-toughened-alumina (ZTA) functionalized with Ag NPs for molecule detection via SERS.…”

Section: Introductionmentioning

confidence: 99%

Gold Nanoparticle‐Coated Bioceramics for Plasmonically Enhanced Molecule Detection via Surface‐Enhanced Raman Scattering

Guo,

Schmidt,

Murshed

et al. 2023

Adv Eng Mater

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In this study we evaluate the feasibility of plasmonically‐enhanced molecule detection via surface‐enhanced Raman scattering (SERS) for ceramics that are commonly used as bone or tooth replacement materials. Open cell foams of Bioglass45S5, the commercial hydroxyapatite‐based product Bio‐Oss® and bio‐inert zirconia‐toughened‐alumina (ZTA) were coated with Au nanoparticles via colloidal deposition to introduce plasmonic effects. Depending on the pore size, gold‐functionalized plasmonic porous Bioglass showed effective Raman enhancement factors (eEF) up to 5.4·104, while depositing gold nanoparticles on Bio‐Oss® and porous ZTA resulted in EFs of 1.1·104 and 2.4·105, respectively. The performance of the plasmonic porous bioceramics under simulated biological conditions was examined in situ in the biological medium fetal bovine serum (FBS) and during extended incubation in mineralizing simulated body fluid (SBF). Most notably, the plasmonic porous Bioglass still delivered an EF around 7.2·103 after 28 days of incubation in SBF, indicating a promising stability in simulated biological conditions without significant difference in SBF‐bioactivity before and after Au‐deposition. Accordingly, the plasmonically‐enhanced porous bioceramics offer the possibility for real‐time and sensitive molecule detection at SBF and FBS conditions and could be further developed for sensing of specific biomarkers, e.g. in the context of osseointegration of bone replacement materials. Beyond biomedical applications, the plasmonically‐enhanced ceramics can be used for biotechnological applications featuring in situ monitoring of molecule turn‐over rates within porous reactors.This article is protected by copyright. All rights reserved.

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Section: Introductionmentioning

confidence: 99%

Gold Nanoparticle‐Coated Bioceramics for Plasmonically Enhanced Molecule Detection via Surface‐Enhanced Raman Scattering

Guo,

Schmidt,

Murshed

et al. 2023

Adv Eng Mater

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“…SUB systems are currently an often used alternative and cover a broad range of different applications despite lacking many conventional engineering parameters or availability of compatible single-use sensors [4,5]. In the context of modern bioprocess development and optimization, the online monitoring of crucial key performance indicators (KPIs) is necessary to ensure high process and product quality [6]. Therefore, monitoring sensors that can be applied to SUB systems and deliver high-grade, real-time information is a significant need, especially for one of the most valuable indicators, the biomass concentration [7,8].…”

Section: Introductionmentioning

confidence: 99%

Off-Gas-Based Soft Sensor for Real-Time Monitoring of Biomass and Metabolism in Chinese Hamster Ovary Cell Continuous Processes in Single-Use Bioreactors

Wallocha

Popp

2021

Processes

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In mammalian cell culture, especially in pharmaceutical manufacturing and research, biomass and metabolic monitoring are mandatory for various cell culture process steps to develop and, finally, control bioprocesses. As a common measure for biomass, the viable cell density (VCD) or the viable cell volume (VCV) is widely used. This study highlights, for the first time, the advantages of using VCV instead of VCD as a biomass depiction in combination with an oxygen-uptake- rate (OUR)-based soft sensor for real-time biomass estimation and process control in single-use bioreactor (SUBs) continuous processes with Chinese hamster ovary (CHO) cell lines. We investigated a series of 14 technically similar continuous SUB processes, where the same process conditions but different expressing CHO cell lines were used, with respect to biomass growth and oxygen demand to calibrate our model. In addition, we analyzed the key metabolism of the CHO cells in SUB perfusion processes by exometabolomic approaches, highlighting the importance of cell-specific substrate and metabolite consumption and production rate qS analysis to identify distinct metabolic phases. Cell-specific rates for classical mammalian cell culture key substrates and metabolites in CHO perfusion processes showed a good correlation to qOUR, yet, unexpectedly, not for qGluc. Here, we present the soft-sensoring methodology we developed for qPyr to allow for the real-time approximation of cellular metabolism and usage for subsequent, in-depth process monitoring, characterization and optimization.

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Measurement Technologies for Upstream and Downstream Bioprocessing

Abstract: This special issue is devoted to new developments in measurement technologies for upstream and downstream bioprocessing [...]

Cited by 2 publications

References 15 publications

Gold Nanoparticle‐Coated Bioceramics for Plasmonically Enhanced Molecule Detection via Surface‐Enhanced Raman Scattering

Gold Nanoparticle‐Coated Bioceramics for Plasmonically Enhanced Molecule Detection via Surface‐Enhanced Raman Scattering

Off-Gas-Based Soft Sensor for Real-Time Monitoring of Biomass and Metabolism in Chinese Hamster Ovary Cell Continuous Processes in Single-Use Bioreactors

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