Measurement of Urinary Level of a Specific Competing endogenous RNA network (FOS and RCAN mRNA/ miR-324-5p, miR-4738-3p, /lncRNA miR-497-HG) Enables Diagnosis of Bladder Cancer

Eissa, Sanaa; Safwat, Maheera  H.; Matboli, Marwa; zaghloul, Ashraf; El‐Sawalhi, Maha M.; Shaheen, Amira

doi:10.1016/j.urolonc.2018.12.024

Cited by 31 publications

(40 citation statements)

References 36 publications

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“…Although significant results regarding miRNAs as diagnostic tools have been proposed in both blood-derived and urine biospecimen separately, a higher accuracy might be achieved by combining measurements of different classes of ncRNAs from different body fluids. Indeed, some authors have already proposed diagnostic biomarker combinations of different RNA classes [60], with excellent results. Recent advances in this field are promising and await translation into clinical practice.…”

Section: Discussionmentioning

confidence: 99%

Small Non-Coding RNA Profiling in Plasma Extracellular Vesicles of Bladder Cancer Patients by Next-Generation Sequencing: Expression Levels of miR-126-3p and piR-5936 Increase with Higher Histologic Grades

Sabo

Birolo

Naccarati

et al. 2020

Cancers

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Bladder cancer (BC) is the tenth most frequent cancer worldwide. Due to the need for recurrent cystoscopies and the lack of non-invasive biomarkers, BC is associated with a high management burden. In this respect, small non-coding RNAs (sncRNAs) have been investigated in urine as possible biomarkers for BC, but in plasma their potential has not yet been defined. The expression levels of sncRNAs contained in plasma extracellular vesicles (EVs) from 47 men with BC and 46 healthy controls were assessed by next-generation sequencing. The sncRNA profiles were compared with urinary profiles from the same subjects. miR-4508 resulted downregulated in plasma EVs of muscle-invasive BC patients, compared to controls (adj-p = 0.04). In World Health Organization (WHO) grade 3 (G3) BC, miR-126-3p was upregulated both in plasma EVs and urine, when compared to controls (for both, adj-p < 0.05). Interestingly, two sncRNAs were associated with the risk class: miR-4508 with a downward trend going from controls to high risk BC, and piR-hsa-5936 with an upward trend (adj-p = 0.04 and adj-p = 0.05, respectively). Additionally, BC cases with low expression of miR-185-5p and miR-106a-5p or high expression of miR-10b-5p showed shorter survival (adj-p = 0.0013, adj-p = 0.039 and adj-p = 0.047, respectively). SncRNAs from plasma EVs could be diagnostic biomarkers for BC, especially in advanced grade.

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Section: Discussionmentioning

confidence: 99%

Small Non-Coding RNA Profiling in Plasma Extracellular Vesicles of Bladder Cancer Patients by Next-Generation Sequencing: Expression Levels of miR-126-3p and piR-5936 Increase with Higher Histologic Grades

Sabo

Birolo

Naccarati

et al. 2020

Cancers

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“…BC patients presented higher expression levels of miR-324-5p, miR-4738-3p, and FBJ murine osteosarcoma viral oncogene homolog B (FOSB) mRNA, and lower expression levels of lncRNA miR-497-HG and Regulator of Calcineurin 1 (RCAN1) mRNA on voided urine. When directly compared with cytology that presented an accuracy of 69.9%, the potential biomarker with lowest accuracy was lncRNA miR-497-HG with 78.3% and the highest, with 99.3% accuracy, were FOSB mRNA and RCAN1 mRNA, with a specificity of 98.9%, exhibited more sensitivity than cytology [89].…”

Section: Lncrnas Biomarkersmentioning

confidence: 97%

“…Eissa et al [89] evaluated the expression levels of several miRNAs, lncRNAs, and gene expression in the plasma and voided urine of patients with BC, patients with benign urological conditions, such as benign prostatic hyperplasia or bilharzial cystitis, and healthy controls. BC patients presented higher expression levels of miR-324-5p, miR-4738-3p, and FBJ murine osteosarcoma viral oncogene homolog B (FOSB) mRNA, and lower expression levels of lncRNA miR-497-HG and Regulator of Calcineurin 1 (RCAN1) mRNA on voided urine.…”

Section: Lncrnas Biomarkersmentioning

confidence: 99%

Biomarkers for Bladder Cancer Diagnosis and Surveillance: A Comprehensive Review

et al. 2020

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Bladder cancer (BC) ranks as the sixth most prevalent cancer in the world, with a steady rise in its incidence and prevalence, and is accompanied by a high morbidity and mortality. BC is a complex disease with several molecular and pathological pathways, thus reflecting different behaviors depending on the clinical staging of the tumor and molecular type. Diagnosis and monitoring of BC is mainly performed by invasive tests, namely periodic cystoscopies; this procedure, although a reliable method, is highly uncomfortable for the patient and it is not exempt of comorbidities. Currently, there is no formal indication for the use of molecular biomarkers in clinical practice, even though there are several tests available. There is an imperative need for a clinical non-invasive testing for early detection, disease monitoring, and treatment response in BC. In this review, we aim to assess and compare different tests based on molecular biomarkers and evaluate their potential role as new molecules for bladder cancer diagnosis, follow-up, and treatment response monitoring.

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“…MIR497HG is a host gene of the miR-497 and miR-195 embedded in its rst intron. MIR497HG was signi cantly downregulated and might serve as a potential diagnostic marker in BCa [15]. MiR-495 and miR-195 were also reduced in BCa and inhibited cancer cell progression [16,37,38].…”

Section: Discussionmentioning

confidence: 96%

“…MiR-17 ~ 92 cluster miRNAs encoded by MIR17HG downregulates TGF-β and STAT3 signaling in feingold syndrome mouse models [14]. LncRNA MIR497HG and derived miR-497 ~ 195 cluster are downregulated in BCa [15,16].…”

Section: Introductionmentioning

confidence: 99%

LncRNA MIR497HG suppresses bladder cancer progression through disrupting the crosstalk between Hippo/Yap and TGF-β/Smad signaling

Zhuang

Liu

Yuan

et al. 2020

Preprint

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Objectives A subclass of long non-coding RNAs (lncRNAs), categorized as miRNA-host gene lncRNAs (lnc-miRHGs), is processed to produce miRNAs and involve in cancer progression. This work aimed to investigate the influence and the molecular mechanisms of lnc-miRHGs MIR497HG in bladder cancer (BCa). Materials and methods The miR-497 and miR-195 were derived from MIR497HG. Cell proliferation, migration and invasion assays were used to measure the function of MIR497HG, miR-497 and miR-195 in BCa. Bioinformatics, RT-qPCR, western blot, luciferase reporter assay, ChIP, and so on, were used to reveal the upstream and downstream mechanisms of MIR497HG in BCa. Results We identified that lnc-miRHG MIR497HG and two harbored miRNAs, miR-497 and miR-195, were downregulated in BCa by analyzing TCGA and our dataset. MIR497HG overexpression inhibited BCa cell proliferation, migration and invasion in vitro. MiR-497/miR-195 inhibitor rescued significantly the inhibiton effects of overexpression of MIR497HG in BCa. Mechanistically, miR-497 and miR-195 coordinately suppressed multiple key components in Hippo/Yap and TGF-β signaling, and particularly attenuated the interaction between Yap and Smad3. In addition, E2F4 was proved to be critical for silencing MIR497HG transcription in BCa cells. Conclusions We propose for the first time that MIR497HG suppressed BCa progression and its upstream and downstream mechanisms. Blocking the pathological process may be a potential strategy for the treatment of BCa.

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Measurement of Urinary Level of a Specific Competing endogenous RNA network (FOS and RCAN mRNA/ miR-324-5p, miR-4738-3p, /lncRNA miR-497-HG) Enables Diagnosis of Bladder Cancer

Cited by 31 publications

References 36 publications

Small Non-Coding RNA Profiling in Plasma Extracellular Vesicles of Bladder Cancer Patients by Next-Generation Sequencing: Expression Levels of miR-126-3p and piR-5936 Increase with Higher Histologic Grades

Small Non-Coding RNA Profiling in Plasma Extracellular Vesicles of Bladder Cancer Patients by Next-Generation Sequencing: Expression Levels of miR-126-3p and piR-5936 Increase with Higher Histologic Grades

Biomarkers for Bladder Cancer Diagnosis and Surveillance: A Comprehensive Review

LncRNA MIR497HG suppresses bladder cancer progression through disrupting the crosstalk between Hippo/Yap and TGF-β/Smad signaling

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