Measurement of urinary arsenic profiles and DNA hypomethylation in a case–control study of urothelial carcinoma

Chung, Chi-Jung; Lee, Hui‐Ling; Chang, Chao‐Hsiang; Han, Chang; Liu, Chiu-Shong; Jung, Wei-Ting; Liu, Huei-Ju; Liou, Saou-Hsing; Chung, Mu‐Chi; Hsueh, Yu Mei

doi:10.1007/s00204-019-02500-y

Cited by 18 publications

(23 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results clearly showed that the contents of iAs, MMA, and DMA in the exposed group were much higher than those in the control group. The urinary arsenic level of workers in our study was higher than that of people exposed to the environment, 4,21,24,35 which was consistent with previous occupational exposure studies. 9 Our study showed that MMA% and iA% were higher and DMA% was lower in the exposed group.…”

Section: Discussionsupporting

confidence: 92%

“…Some studies had shown that individuals with low methylation ability were distinguished by lower urine SMI, were more likely to suffer from arsenicrelated diseases. [1][2][3]21,26,37 Our data suggested that subjects with low methylation ability had significantly higher levels of gene expression, DNA damage, and DNA methylation than those with high metabolic efficiency. Our results are consistent with previous findings that arsenic metabolism affects gene expression levels, DNA damage, and DNA methylation.…”

Section: Discussionmentioning

confidence: 64%

“…Thus, iAs might alter the methylation status of genomic DNA and increase the transcription of some genes. [16][17][18][19][20][21] The metabolism of iAs could cause oxidative stress and damage through generating free radicals and active oxygen species, which finally brought about a broad variety of base modification and DNA break. DNA damage was a key initial event in the carcinogenic potential of arsenic.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The ability of arsenic metabolism affected the expression of lncRNA PANDAR, DNA damage, or DNA methylation in peripheral blood lymphocytes of laborers

Zhang

Chen

et al. 2019

Hum Exp Toxicol

View full text Add to dashboard Cite

Arsenic has been associated with significant effects on human health. Exposure to inorganic arsenic has been associated with the changes in gene expression. Promoter of CDKN1A antisense DNA damage activated RNA (PANDAR) expression is induced by p53 protein and DNA damage response. Here, we investigated whether the ability of arsenic metabolism in individuals affected the expression of PANDAR, DNA damage, and DNA methylation. Levels of gene expression and DNA damage were examined by the quantitative polymerase chain reaction and DNA methylation was measured by the methylation-sensitive high-resolution melting curve. In our study, we demonstrated that arsenic exposure increased PANDAR expression and DNA damage among arsenic smelting plant laborers. The PANDAR expression and DNA damage were positively linked to monomethylarsonic acid % ( R = 0.25, p < 0.05 and R = 0.32, p < 0.01) and negatively linked to dimethylarsinic acid % ( R = −0.21, p < 0.05 and R = −0.31, p < 0.01). Subjects with low primary methylation index had increased levels of DNA damage (51.62 ± 2.96 vs. 60.93 ± 3.10, p < 0.05) and methylation (17.14 (15.88–18.51) vs. 15.83 (14.82–18.00), p < 0.05). Subjects with low secondary methylation index had increased levels of PANDAR expression (4.88 ± 0.29 vs. 4.07 ± 0.23, p < 0.01) and DNA damage (17.38 (15.88–19.29) vs. 15.83 (14.82–17.26), p < 0.01). DNA methylation of PANDAR gene was linked to the regulation of its expression in peripheral blood lymphocytes among laborers ( Y = −2.08 × X + 5.64, p < 0.05). These findings suggested arsenic metabolism ability and exposure affected the expression of PANDAR, DNA damage, and DNA methylation.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 64%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The ability of arsenic metabolism affected the expression of lncRNA PANDAR, DNA damage, or DNA methylation in peripheral blood lymphocytes of laborers

Zhang

Chen

et al. 2019

Hum Exp Toxicol

View full text Add to dashboard Cite

show abstract

“…Therefore, both the functional gain of proto-oncogene mutations and loss-offunction mutations in tumor suppressor genes might cause cancer through uncontrolled cell growth and defective apoptosis [22]. DNA methylation involves the addition of a methyl group at the cytosine 5′ carbon position of CpG dinucleotides in the genome, which is an important element of epigenetic regulation of gene expression [23]. Since the 1990s, increasing number of studies have recognized that heritable changes regulated by epigenetics might also play a vital role in the evolution of all types of human cancer [24].…”

Section: Discussionmentioning

confidence: 99%

DNA methylation-based classification and identification of bladder cancer prognosis-associated subgroups

et al. 2020

View full text Add to dashboard Cite

Background: Bladder cancer (BCA) is the most common urinary tumor, but its pathogenesis is unclear, and the associated treatment strategy has rarely been updated. In recent years, a deeper understanding of tumor epigenetics has been gained, providing new opportunities for cancer detection and treatment. Methods: We identified prognostic methylation sites based on DNA methylation profiles of BCA in the TCGA database and constructed a specific prognostic subgroup. Results: Based on the consistent clustering of 402 CpGs, we identified seven subgroups that had a significant association with survival. The difference in DNA methylation levels was related to T stage, N stage, M stage, grade, sex, age, stage and prognosis. Finally, the prediction model was constructed using a Cox regression model and verified using the test dataset; the prognosis was consistent with that of the training set. Conclusions: The classification based on DNA methylation is closely related to the clinicopathological characteristics of BCA and determines the prognostic value of each epigenetic subtype. Therefore, our findings provide a basis for the development of DNA methylation subtype-specific therapeutic strategies for human bladder cancer.

show abstract

“…Therefore, both the functional gain of proto-oncogene mutations and loss-of-function mutations in tumor suppressor genes might cause cancer through uncontrolled cell growth and defective apoptosis [22]. DNA methylation involves the addition of a methyl group at the cytosine 5′ carbon position of CpG dinucleotides in the genome, which is an important element of epigenetic regulation of gene expression [23]. Since the 1990s, increasing number of studies have recognized that heritable changes regulated by epigenetics might also play a vital role in the evolution of all types of human cancer [24].…”

Section: Discussionmentioning

confidence: 99%

DNA methylation-based classification and identification of bladder cancer prognosis-associated subgroups

Tian

Meng

Long

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Bladder cancer (BCA) is the most common urinary tumor, but its pathogenesis is unclear, and the associated treatment strategy has rarely been updated. In recent years, a deeper understanding of tumor epigenetics has been gained, providing new opportunities for cancer detection and treatment.Methods: We identified prognostic methylation sites based on DNA methylation profiles of BCA in the TCGA database and constructed a specific prognostic subgroup. Results: Based on the consistent clustering of 402 CpGs, we identified seven subgroups that had a significant association with survival. The difference in DNA methylation levels was related to T stage, N stage, M stage, grade, sex, age, stage and prognosis. Finally, the prediction model was constructed using a Cox regression model and verified using the test dataset; the prognosis was consistent with that of the training set. Conclusions: The classification based on DNA methylation is closely related to the clinicopathological characteristics of BCA and determines the prognostic value of each epigenetic subtype. Therefore, our findings provide a basis for the development of DNA methylation subtype-specific therapeutic strategies for human bladder cancer.

show abstract

Measurement of urinary arsenic profiles and DNA hypomethylation in a case–control study of urothelial carcinoma

Cited by 18 publications

References 39 publications

The ability of arsenic metabolism affected the expression of lncRNA PANDAR, DNA damage, or DNA methylation in peripheral blood lymphocytes of laborers

The ability of arsenic metabolism affected the expression of lncRNA PANDAR, DNA damage, or DNA methylation in peripheral blood lymphocytes of laborers

DNA methylation-based classification and identification of bladder cancer prognosis-associated subgroups

DNA methylation-based classification and identification of bladder cancer prognosis-associated subgroups

Contact Info

Product

Resources

About