Measurement of tryptophan in peptides by acid hydrolysis in the presence of phenol and its application to the amino acid sequence of a sea anemone toxin.

Muramoto, Koji; Sunahara, Satoshi; Kamiya, Hisao

doi:10.1271/bbb1961.51.1607

Cited by 25 publications

(2 citation statements)

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“…The authors have cited additional references within the Supporting Information. [16][17][18][19][20]…”

Section: Supporting Informationmentioning

confidence: 99%

Rapid Unambiguous Structure Elucidation of Streptnatamide A, a New Cyclic Peptide Isolated from A Marine‐derived Streptomyces sp

Yan,

Wu,

Maity

et al. 2023

Chemistry A European J

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Cyclic peptides have been excellent source of drug leads. With the advances in discovery platforms, the pharmaceutical industry has a growing interest in cyclic peptides and has pushed several into clinical trials. However, structural complexity of cyclic peptides brings extreme challenges for structure elucidation efforts. Isotopic fine structure analysis, Nuclear magnetic resonance (NMR), and detailed tandem mass spectrometry rapidly provided peptide sequence for streptnatamide A, a cyclic peptide isolated from a marine‐derived Streptomyces sp. Marfey’s analysis determined the stereochemistry of all amino acids, enabling the unambiguous structure determination of this compound. A non‐ribosomal peptide synthetase biosynthetic gene cluster (stp) was tentatively identified and annotated for streptnatamide A based on the in‐silico analysis of whole genome sequencing data. These analytical tools will be powerful tools to overcome the challenges for cyclic peptide structure elucidation and accelerate the development of bioactive cyclic peptides.

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“…The authors have cited additional references within the Supporting Information. [16][17][18][19][20]…”

Section: Supporting Informationmentioning

confidence: 99%

Rapid Unambiguous Structure Elucidation of Streptnatamide A, a New Cyclic Peptide Isolated from A Marine‐derived Streptomyces sp

Yan,

Wu,

Maity

et al. 2023

Chemistry A European J

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“…Sample hydrolysis was carried out using HCl (6 N) with phenol (2% w/v) to aid removal of oxygen radicals (Muramoto et al, 1987). Freeze-dried supernatant and retentate (10 mg) were digested in HCl (6 N; 10 mL, prepared as described above) and digested using MARS 6 (CEM, US) microwave digester with a temperature ramp to 150 • C for 20 min.…”

Section: Protein Estimationmentioning

confidence: 99%

Invasive Plants Are a Valuable Alternate Protein Source and Can Contribute to Meeting Climate Change Targets

Iyer

Bestwick

Duncan

et al. 2021

Front. Sustain. Food Syst.

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Agriculture has come under pressure to meet global food demands, whilst having to meet economic and ecological targets. This has opened newer avenues for investigation in unconventional protein sources. Current agricultural practises manage marginal lands mostly through animal husbandry, which; although effective in land utilisation for food production, largely contributes to global green-house gas (GHG) emissions. Assessing the revalorisation potential of invasive plant species growing on these lands may help encourage their utilisation as an alternate protein source and partially shift the burden from livestock production; the current dominant source of dietary protein, and offer alternate means of income from such lands. Six globally recognised invasive plant species found extensively on marginal lands; Gorse (Ulex europaeus), Vetch (Vicia sativa), Broom (Cytisus scoparius), Fireweed (Chamaenerion angustifolium), Bracken (Pteridium aquilinum), and Buddleia (Buddleja davidii) were collected and characterised to assess their potential as alternate protein sources. Amino acid profiling revealed appreciable levels of essential amino acids totalling 33.05 ± 0.04 41.43 ± 0.05, 33.05 ± 0.11, 32.63 ± 0.04, 48.71 ± 0.02 and 21.48 ± 0.05 mg/g dry plant mass for Gorse, Vetch, Broom Fireweed, Bracken, and Buddleia, respectively. The availability of essential amino acids was limited by protein solubility, and Gorse was found to have the highest soluble protein content. It was also high in bioactive phenolic compounds including cinnamic- phenyl-, pyruvic-, and benzoic acid derivatives. Databases generated using satellite imagery were used to locate the spread of invasive plants. Total biomass was estimated to be roughly 52 Tg with a protein content of 5.2 Tg with a total essential amino acid content of 1.25 Tg (~24%). Globally, Fabaceae was the second most abundant family of invasive plants. Much of the spread was found within marginal lands and shrublands. Analysis of intrinsic agricultural factors revealed economic status as the emergent factor, driven predominantly by land use allocation, with shrublands playing a pivotal role in the model. Diverting resources from invasive plant removal through herbicides and burning to leaf protein extraction may contribute toward sustainable protein, effective land use, and achieving emission targets, while simultaneously maintaining conservation of native plant species.

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Recovery of tryptophan in peptides and proteins by high-temperature and short-term acid hydrolysis in the presence of phenol

Muramoto

Kamiya

1990

Analytical Biochemistry

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Measurement of tryptophan in peptides by acid hydrolysis in the presence of phenol and its application to the amino acid sequence of a sea anemone toxin.

Cited by 25 publications

References 0 publications

Rapid Unambiguous Structure Elucidation of Streptnatamide A, a New Cyclic Peptide Isolated from A Marine‐derived Streptomyces sp

Rapid Unambiguous Structure Elucidation of Streptnatamide A, a New Cyclic Peptide Isolated from A Marine‐derived Streptomyces sp

Invasive Plants Are a Valuable Alternate Protein Source and Can Contribute to Meeting Climate Change Targets

Recovery of tryptophan in peptides and proteins by high-temperature and short-term acid hydrolysis in the presence of phenol

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