Measurement of transferrin in human seminal plasma by a chemiluminescent method.

Caldini, Anna; Orlando, Claudio; Barni, Tullio; Messeri, Gianni; Pazzagli, Mario; Baldi, E; Serio, Mario

doi:10.1093/clinchem/32.1.153

Cited by 15 publications

(3 citation statements)

References 4 publications

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“…The results we obtained for immunochemically reactive albumin in the semen of vasectomized men suggest that the testis is the principal source of seminal albumin, as previously demonstrated for transferrin (Orlando et al, 1985;Caldini et al, 1986). The decrease in seminal values of albumin in oligozoospermic and azoospermic patients was less marked than that found for transferrin, suggesting that the secretion of albumin is a process that is less sensitive to Sertoli cell impairment.…”

Section: Discussionsupporting

confidence: 76%

Immunologically reactive albumin-like protein in human testis and seminal plasma

Orlando¹,

Casano²,

Forti³

et al. 1988

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An immunologically reactive albumin-like protein (albumin) was localized, by an immunostaining technique, in the testis of infertile men (normal spermatogenesis, obstructive azoospermia) at the level of the Sertoli cells and in some cells of the germinal epithelium (secondary spermatocytes and early spermatids). No positive reaction was detectable in prepubertal testis. In vasectomized men, mean seminal albumin values were drastically reduced (by about 80%) in comparison to fertile controls, indicating a probable testicular origin. Mean seminal albumin values were also decreased in patients affected by azoospermia due to a seminiferous tubular lesion (about 40%) and in oligozoospermic patients (about 30%). In the same seminal samples transferrin, an index of Sertoli cell function, was also measured. Albumin and transferrin results were well correlated in the seminal plasma of each group (with the exception of vasectomized subjects), including a group of men with abnormally high concentrations of seminal transferrin. A weak correlation was found between seminal albumin and sperm count. We suggest that the presence of albumin in the human adult testis and in seminal plasma could be related to its ability to transport androgens.

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Section: Discussionsupporting

confidence: 76%

Immunologically reactive albumin-like protein in human testis and seminal plasma

Orlando¹,

Casano²,

Forti³

et al. 1988

Reproduction

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“…Previous reports from this (Orlando et al, 1985, Caldini et al, 1986 and other laboratories (Holmes, Lipshultz & Smith, 1982) have demonstrated that, in man, 80% of seminal transferrin derives from the Sertoli cell. Moreover, seminal transferrin is below the normal range in patients with azoospermia resulting from severe seminiferous tubule damage without obstruction.…”

Section: Introductionmentioning

confidence: 56%

“…Moreover, seminal transferrin is below the normal range in patients with azoospermia resulting from severe seminiferous tubule damage without obstruction. In the same studies (Orlando et al, 1985, Caldini et al, 1986 a significant correlation was found between seminal transferrin levels and sperm number. Therefore, seminal transferrin may be a useful clinical index of seminiferous tubule function.…”

Section: Introductionmentioning

confidence: 63%

Immunoassayable Somatomedin C in seminal plasma of azoospermic men

et al. 1987

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Somatomedin C is a Sertoli cell peptide and since measurements of other Sertoli cell products in semen have provided a useful indices of testicular function, it was considered pertinent to measure the semen levels of Somatomedin C. Somatomedin C was measured by RIA in seminal plasma of vasectomized subjects (n = 18), subjects with agenesis of the seminal vesicles and vasa deferentia (n = 6) and subjects with azoospermia resulting from seminiferous tubule damage without obstruction (n = 23). Normal fertile subjects (24 men with a sperm concentration > 20 × 106/ml) were used as controls. In all subjects, seminal levels of transferrin were also measured as an index of Sertoli cell function. The majority of seminal Somatomedin C appears to derive from the testis and/or epididymis. However, in several normal controls seminal levels of Somatomedin C (median = 3.52; range = 1.10–15.67 U/ejaculate) were found to be within the range for vasectomized subjects (median = 0.78; range = 0.46–4.20 U/ejaculate). In subjects with azoospermia the seminal levels of Somatomedin C (median = 2.06; range = 0.60–10.12 U/ejaculate) were significantly lower (P < 0.02) than in fertile controls. However, values for these two groups overlapped. It is concluded that Somatomedin C in semen is not a reliable index of seminiferous tubule function and does not appear to be of diagnostic value in male infertility.

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Luminescent immunoassay using isoluminol derivatives

Messeri¹,

Orlandini²,

Pazzagli³

1989

J. Biolumin. Chemilumin.

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Isoluminol derivatives (ID) were early employed in the preparation of tracers for immunoassay. Their wide use was mainly due to their high quantum efficiency, low molecular weight, well-known chemical structure, low cost and high stability. Moreover the light efficiency of some ID may be modified by specific binding to the antibody, thus allowing the development of homogenous immunoassays requiring no bound/free separation step. Some ID are commercially available under both the amino terminal and carboxyl terminal form for conjugation to respectively carboxy derivative of steroid and amino residues of protein. The linking reaction can be commonly carried on via active esters chemistry and can be easily accomplished within one day. Steroid conjugates can then be rapidly purified by silica gel thin layer chromatography, and protein conjugates by gel filtration on a short disposable column. In the field of steroid studies, isoluminol derivative conjugates were prepared for the immunoassay of almost all compounds of clinical interest. When dealing with protein, both antigens and antibody were labelled in this way, resulting in highly specific activity tracers for competitive and non-competitive immunoassays. Recently the labelling of streptavidin with amino-buty-ethyl-isoluminol allowed the development of very sensitive immunoassay methods which take advantage of the biotin-avidin system.

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Measurement of transferrin in human seminal plasma by a chemiluminescent method.

Cited by 15 publications

References 4 publications

Immunologically reactive albumin-like protein in human testis and seminal plasma

Immunologically reactive albumin-like protein in human testis and seminal plasma

Immunoassayable Somatomedin C in seminal plasma of azoospermic men

Luminescent immunoassay using isoluminol derivatives

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