Measurement of Trace Elements in Proteins Extracted from Liver by Size Exclusion Chromatography-Inductively Coupled Plasma-Mass Spectrometry with a Magnetic Sector Mass Spectrometer

Wang, Jin; Dreessen, Dawn; Wiederin, Daniel R.; Houk, R. S.

doi:10.1006/abio.2000.4885

Cited by 36 publications

(19 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It means that iron overload has not any dominant impact on hepatic copper content, but can change the distribution of Cu in mouse liver, especially in mitochondria. Wang et al (2001) found that most of copper exists in the smaller protein fractions in bovine liver extraction. But in our experiment, we found that proteins which contain copper are different in different mouse liver subcellular fractions.…”

Section: Discussionmentioning

confidence: 99%

Hepatic distribution of iron, copper, zinc and cadmium-containing proteins in normal and iron overload mice

et al. 2008

View full text Add to dashboard Cite

Subcellular distribution of metal-containing proteins of Fe, Cu, Zn and Cd were determined in the liver samples of iron overload mice by size exclusion high performance liquid chromatography with on-line coupling to UV and inductively coupled plasma mass spectrometry. Collision cell techniques was used to remove polyatomic interferences for some elements, such as Fe. Comparative molecular weight (MW) information of the elemental fraction was obtained within a retention time of 40 min. Fe was present only in high-MW (HMW) protein; Cu, Zn and Cd were found in different MW proteins. It was also observed that these four elements studied showed predominant association with HMW fractions. Moreover, compared with the normal group, we found that the contents of these elements except Cu significantly increased and the distribution of some elements like Cd changed in iron overload mouse liver. It means that excessive iron accumulation in vivo may affect the metabolism of other element such as Zn and Cd.

show abstract

Section: Discussionmentioning

confidence: 99%

Hepatic distribution of iron, copper, zinc and cadmium-containing proteins in normal and iron overload mice

et al. 2008

View full text Add to dashboard Cite

show abstract

“…Speciation analysis of MTs and MLPs by HPLC coupled to ICP-MS seems to be adequate for detecting metal-biomolecule complexes in cytosols from animal tissues [48,49,50,51,52,53]. The majority of such studies concern the speciation of Cd, Zn and Cu in kidney [54,55,56], liver [46,49,57,58,59,60,61,62,63,64] and brain [65] of mammals (rat, rabbit, bovine, humans) or tissues of bream [66], carps [67], eels [68] and mussels [69,70,71]. MT isoforms have been separated by size SEC, anionic exchange-HPLC and reverse phase (RP)-HPLC chromatography.…”

Section: Animal Tissuesmentioning

confidence: 99%

Trace element speciation by ICP-MS in large biomolecules and its potential for proteomics

Sanz‐Medel

Montes-Bayón²,

Sánchez³

2003

Analytical and Bioanalytical Chemistry

152

View full text Add to dashboard Cite

Latest studies on the chemical association of trace elements to large biomolecules and their importance on the bioinorganic and clinical fields are examined. The complexity of the speciation of metal-biomolecules associations in various biological fluids is stressed. Analytical strategies to tackle speciation analysis and the-state-of-the-art of the instrumentation employed for this purpose are critically reviewed. Hyphenated techniques based on coupling chromatographic separation techniques with ICP-MS detection are now established as the most realistic and potent analytical tools available for real-life speciation analysis. Therefore, the status and potential of metal and semimetals elemental speciation in large biocompounds using ICP-MS detection is mainly focused here by reviewing reported metallo-complexes separations using size-exclusion (SEC), ion-exchange (IE), reverse phase chromatography (RP) and capillary electrophoresis (CE). Species of interest include coordination complexes of metals with larger proteins (e.g. in serum, breat milk, etc.) and metallothioneins (e.g. in cytosols from animals and plants) as well as selenoproteins (e.g. in nutritional supplements), DNA-cisplatin adducts and metal/semimetal binding to carbohydrates. An effort is made to assess the potential of present trace elements speciation knowledge and techniques for "heteroatom-tagged" (via ICP-MS) proteomics.

show abstract

“…Quantification was carried out by calibration with a column by-passinjection of elemental standards. Wang et al [49] detected a metallothionein fraction in a bovine liver standard refer-448 ence material using SE-HPLC coupled to an ICP-sector field-MS. With this method, polyatomic interferences could be avoided at medium spectral resolution, and direct detection of sulfur enabled the assignment of the MT fraction. An ICP-sector field-MS was also used by Ferarello et al [50] for the determination of metals in SE-HPLC fractions from a mussel cytosol, in order to estimate metal distribution patterns in biomolecules of different sizes and their possible relationship with environmental and heavy metal contamination.…”

Section: Size-exclusion-hplcmentioning

confidence: 99%

Hyphenated techniques for the characterization and quantification of metallothionein isoforms

Prange¹,

Schaumlöffel²

2002

Anal Bioanal Chem

115

View full text Add to dashboard Cite

Recent developments in the coupling of highly selective separation techniques such as capillary electrophoresis (CE) and high-performance liquid chromatography (HPLC) to element-specific and molecule-specific detectors, such as inductively-coupled plasma mass spectrometry (ICP-MS) and electrospray ionization-tandem mass spectrometry (ESI-MS/MS) for the characterization and quantification of metallothioneins (MTs) are critically reviewed and discussed. This review gives an update based on the literature over the last five years. The coupling of CE to ICP-MS is especially highlighted. As a result of progress in new interface technologies for CE-ICP-MS, research topics presented in the literature are changing from "the characterization of interfaces by metallothioneins" to the "characterization of metallothioneins by CE-ICP-MS". New applications of CE-ICP-MS to the analysis of MTs in real samples are summarized. The potential of the on-line isotope dilution technique for the quantification of MTs and for the determination of the stoichiometric composition of metalloprotein complexes is discussed. Furthermore, a selection of relevant papers dealing with HPLC-ICP-MS for MT analysis are summarized and compared to those dealing with CE-ICP-MS. In particular, the use of size-exclusion (SE)-HPLC as a preliminary separation step for metallothioneins in real samples prior to further chromatographic or electrophoretic separations is considered. Additionally, the application of electrospray ionisation-tandem mass spectrometry (ESI-MS/MS) for the identification of metallothionein isoforms following electrophoretic or chromatographic separation is discussed.

show abstract

Measurement of Trace Elements in Proteins Extracted from Liver by Size Exclusion Chromatography-Inductively Coupled Plasma-Mass Spectrometry with a Magnetic Sector Mass Spectrometer

Cited by 36 publications

References 18 publications

Hepatic distribution of iron, copper, zinc and cadmium-containing proteins in normal and iron overload mice

Hepatic distribution of iron, copper, zinc and cadmium-containing proteins in normal and iron overload mice

Trace element speciation by ICP-MS in large biomolecules and its potential for proteomics

Hyphenated techniques for the characterization and quantification of metallothionein isoforms

Contact Info

Product

Resources

About