Measurement of the distribution of red blood cell deformability using an automated rheoscope

Dobbe, Johannes G. G.; Streekstra, Geert J.; Hardeman, Max R.; İnce, Can; Grimbergen, C.A.

doi:10.1002/cyto.10171

Cited by 88 publications

(84 citation statements)

References 25 publications

(28 reference statements)

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“…Since the importance of deformability has became apparent, it has been measured by various experimental methods (Couette device: Dobbe et al 2002;Micropippet: Evans 1983;Paulitschke andNash 1993, Optical tweezers: Dao et al 2003). From the viewpoint of the flow type ( Fig.…”

Section: Introductionmentioning

confidence: 99%

Extensional flow-based assessment of red blood cell deformability using hyperbolic converging microchannel

Lee

Yim

Ahn

et al. 2009

Biomed Microdevices

134

136

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The deformability of the red blood cell (RBC), is known to be closely related to microcirculation and diagnosis of specific diseases such as malaria, arterial sclerosis, sepsis, and so on. From the viewpoint of the flow type, conventional methods to measure the cell deformability have exploited simple shear or complex flow field with little focus on extensional flow field. In this paper, we present a new approach to assess cell deformability under the extensional flow field. For this purpose, a hyperbolic converging microchannel was designed, and the cell deformation in the extensional flow region was continuously monitored. It overcomes the limitation of conventional methods by reducing experiment time. As quantified by the degree of deformation, the extensional flow (Deformation Index = 0.51 at 3.0 Pa) was found to be more efficient in inducing cell deformation compared to the shear flow (Deformation Index = 0.29 at 3.0 Pa). This indicates the insufficiency of the existing models that predict the blood damage in artificial organs, which only consider shear flow. Also, this method could detect the heat-induced difference in deformability of RBCs. It provides a new platform to study the clinical effect of RBC deformability under extensional flow, and is expected to contribute the association of several diseases and deformability of RBCs.

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Section: Introductionmentioning

confidence: 99%

Extensional flow-based assessment of red blood cell deformability using hyperbolic converging microchannel

Lee

Yim

Ahn

et al. 2009

Biomed Microdevices

134

136

View full text Add to dashboard Cite

show abstract

“…RBC deformability was measured by the Automated Rheoscope and Cell Analyzer (ARCA), in which cells were elongated by shear flow in a viscous medium between parallel glass plates [15]. Advanced imaging algorithms were used to locate cells and to calculate cellular deformability.…”

Section: Methodsmentioning

confidence: 99%

Partial pyruvate kinase deficiency aggravates the phenotypic expression of band 3 deficiency in a family with hereditary spherocytosis

et al. 2014

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In a family with mild dominant spherocytosis, affected members showed partial band 3 deficiency. The index patient showed more severe clinical symptoms than his relatives, and his red blood cells displayed concomitant low pyruvate kinase activity. We investigated the contribution of partial PK deficiency to the phenotypic expression of mutant band 3 in this family. Pyruvate kinase deficiency and band 3 deficiency were characterized by DNA analysis. Results of red cell osmotic fragility testing, the results of cell deformability obtained by the Automated Rheoscope and Cell Analyzer and the results obtained by Osmotic Gradient Ektacytometry, which is a combination of these tests, were related to the red cell ATP content. Spherocytosis in this family was due to a novel heterozygous mutation in SLC4A1, the gene for band 3. Reduced PK activity of the index patient was attributed to a novel mutation in PKLR inherited from his mother, who was without clinical symptoms. Partial PK deficiency was associated with decreased red cell ATP content and markedly increased osmotic fragility. This suggests an aggravating effect of low ATP levels on the phenotypic expression of band 3 deficiency.Am. J. Hematol. 90:E35-E39,

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“…The outer cylinder is rotated by a step motor. As the height of both cylinders signi¯cantly exceeds the width d of the rheologic gap, the distribution of°ow velocities in the radial direction is 8,9 ( Fig. 2).…”

Section: The Ektacytometermentioning

confidence: 99%

Study of laser beam scattering by inhomogeneous ensemble of red blood cells in a shear flow

Nikitin

Луговцов

Ustinov

et al. 2015

J. Innov. Opt. Health Sci.

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Laser ektacytometry is a technique widely used for measuring the deformability of red blood cells (erythrocytes) in blood samples in vitro. In ektacytometer, a°ow of highly diluted suspension of erythrocytes in variable shear stress conditions is illuminated with a laser beam to obtain a di®raction pattern. The di®raction pattern provides information about the shapes (shear-induced elongations) of the cells under investigation. This paper is dedicated to developing the technique of laser ektacytometry so that it would enable one to measure the distribution of the erythrocytes in deformability. We discuss the problem of calibration of laser ektacytometer and test a novel data processing algorithm allowing to determine the parameters of the distribution of erythrocytes deformability. Experimentally, we examined 12 specimens of blood of rats under the action of 4 shear stresses. Analysis of the data shows that in conditions of a limited range of digitizing the di®raction patterns, the measurement errors for the mean deformability, deformability scatter and the skewness of erythrocytes distribution in deformability by our method are respectively 15%, 20% and 20%.

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Measurement of the distribution of red blood cell deformability using an automated rheoscope

Cited by 88 publications

References 25 publications

Extensional flow-based assessment of red blood cell deformability using hyperbolic converging microchannel

Extensional flow-based assessment of red blood cell deformability using hyperbolic converging microchannel

Partial pyruvate kinase deficiency aggravates the phenotypic expression of band 3 deficiency in a family with hereditary spherocytosis

Study of laser beam scattering by inhomogeneous ensemble of red blood cells in a shear flow

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