Measurement of the Band Broadening of UV Detectors used in Ultra-high Performance Liquid Chromatography using an On-tubing Fluorescence Detector

Abstract

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Advances in ultra‐high‐pressure and multi‐dimensional liquid chromatography instrumentation and workflows

“…Recent investigations by Dasgupta et al 95 . and Vanderlinden et al 96 . have shown that the dispersion contribution by the detector cell is also slightly affected by the applied flow rate, and is a function of flow cell volume.…”

Reversed-Phase Liquid Chromatography of Peptides for Bottom-Up Proteomics: A Tutorial

“…The volumetric contribution of the detector cell to the total band broadening depends on the volume of the detector cell ( V cell ) and a constant characteristic of the UV cell geometry ( k cell ) (eq ). σ italicdet 2 = V c e l l 2 k c e l l The value k cell was previously reported to lie between 1 and 12, but for modern analytical ultrahigh performance liquid chromatography (UHPLC) systems with small volume cells, values around 1 are more realistic . The capillaries connecting the UV detector increase significantly the total length that a band eluting from a column has to pass to reach the mass spectrometer.…”

Extra-column band broadening effects in contemporary liquid chromatography: Causes and solutions

“…Using a modified detector that allowed continuous variation of the cell path length and thus detection volume, it was recently clearly illustrated that flow cell dispersion is flow rate dependent unless very long path lengths are considered [95]. For short path lengths, the flow profiles at the inlet and outlet of the outlet of the detector start to make out the majority of the dispersion rather than the cell volume and det < 1 can easily be observed [95,96].…”