Measurement of superoxide dismutase, catalase and glutathione peroxidase in cultured cells and tissue

Weydert, Christine J.; Cullen, Joseph J.

doi:10.1038/nprot.2009.197

Cited by 1,131 publications

(824 citation statements)

References 42 publications

(55 reference statements)

Supporting

Mentioning

785

Contrasting

Unclassified

Order By: Relevance

“…SOD enzyme converts superoxide radicals into molecular oxygen and hydrogen peroxide, while CAT converts hydrogen peroxide into water and oxygen (Firuzi et al, 2011;Weydert and Cullen, 2010). In the present study, the liver of both SOD and CAT enzyme activities were significantly lower in the APAP-only treated group.…”

mentioning

confidence: 48%

Protective effect of Panax ginseng against N-acetyl-p-aminophenol-induced hepatotoxicity in rats

Uzkeser¹

2012

Afr. J. Pharm. Pharmacol.

View full text Add to dashboard Cite

Panax ginseng (PG) has been commonly used as medicinal herb for the treatment of various diseases in Eastern Asia for thousands of years. This study was designed to investigate the protective effect of PG against paracetamol-(N-acetyl-p-aminophenol; APAP)-induced liver damage in rats. Thirty-two albino Wistar rats were divided into four groups: Group C (control); Group APAP (gavaged orally with APAP (APAP, 2 g/kg, single dose); Group PG 100 mg/kg + APAP or 200 mg/kg + APAP (these two groups were treated by gavaging with PG (100 or 200 mg/kg) for 30 days following a single dose of APAP). APAP treatment alone induced hepatotoxicity, evidenced by significant increases in malondialdehyde (MDA) level and serum activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and tumor necrosis factor-α (TNF-α). In addition, the level of GSH in the liver was significantly reduced, as were the activities of superoxide dismutase (SOD) and catalase (CAT). Liver histopathological examination showed that APAP administration induced centrilobular necrosis and infiltration of lymphocytes. However, these biochemical and histological changes were prevented by PG pretreatment. In conclusion, our results suggest that PG may be considered a protective medicinal herb against APAP-induced liver injury.

show abstract

mentioning

confidence: 48%

Protective effect of Panax ginseng against N-acetyl-p-aminophenol-induced hepatotoxicity in rats

Uzkeser¹

2012

Afr. J. Pharm. Pharmacol.

View full text Add to dashboard Cite

show abstract

“…The levels and enzyme activities of Mn-and CuZn-superoxide dismutases (SOD1 and SOD2) were determined by Western blot analysis and by in-gel activity assay as described previously (68). To determine steady-state levels of these enzymes by Western blotting, we used an anti-SOD1 antibody from Calbiochem and an anti-SOD2 antibody from Upstate.…”

mentioning

confidence: 99%

Cells Lacking Rieske Iron-Sulfur Protein Have a Reactive Oxygen Species-Associated Decrease in Respiratory Complexes I and IV

Díaz

Enríquez

Moraes

2012

Molecular and Cellular Biology

115

View full text Add to dashboard Cite

Mitochondrial respiratory complexes of the electron transport chain (CI, CIII, and CIV) can be assembled into larger structures forming supercomplexes. We analyzed the assembly/stability of respiratory complexes in mouse lung fibroblasts lacking the Rieske iron-sulfur protein (RISP knockout [KO]cells), one of the catalytic subunits of CIII. In the absence of RISP, most of the remaining CIII subunits were able to assemble into a large precomplex that lacked enzymatic activity. CI, CIV, and supercomplexes were decreased in the RISP-deficient cells. Reintroduction of RISP into KO cells restored CIII activity and increased the levels of active CI, CIV, and supercomplexes. We found that hypoxia (1% O 2 ) resulted in increased levels of CI, CIV, and supercomplex assembly in RISP KO cells. In addition, treatment of control cells with different oxidative phosphorylation (OXPHOS) inhibitors showed that compounds known to generate reactive oxygen species (ROS) (e.g., antimycin A and oligomycin) had a negative impact on CI and supercomplex levels. Accordingly, a superoxide dismutase (SOD) mimetic compound and SOD2 overexpression provided a partial increase in supercomplex levels in the RISP KO cells. Our data suggest that the stability of CI, CIV, and supercomplexes is regulated by ROS in the context of defective oxidative phosphorylation.

show abstract

“…SOD converts superoxide radicals into hydrogen peroxide and molecular oxygen (O 2 ), while the CAT and POD convert hydrogen peroxide into water, and in the case of CAT, to oxygen and water [45]. Capsaicinoids are products of the phenylpropanoid and fatty acid synthesis pathways [46].…”

Section: Tdz) Vertical Bars Represent Se (N=3) Same Letters Arementioning

confidence: 99%

Effect of Thidiazuron used as an Elicitor in the Production of Capsaicin Ontotal Protein and Phenolic Amounts, Antioxidant Enzyme Activities of Pepper Plants

İşlek¹,

Ünal²,

Koç³

et al. 2016

IJEAB

View full text Add to dashboard Cite

show abstract

Measurement of superoxide dismutase, catalase and glutathione peroxidase in cultured cells and tissue

Cited by 1,131 publications

References 42 publications

Protective effect of Panax ginseng against N-acetyl-p-aminophenol-induced hepatotoxicity in rats

Protective effect of Panax ginseng against N-acetyl-p-aminophenol-induced hepatotoxicity in rats

Cells Lacking Rieske Iron-Sulfur Protein Have a Reactive Oxygen Species-Associated Decrease in Respiratory Complexes I and IV

Effect of Thidiazuron used as an Elicitor in the Production of Capsaicin Ontotal Protein and Phenolic Amounts, Antioxidant Enzyme Activities of Pepper Plants

Contact Info

Product

Resources

About