Measurement of some Benzylisoquinoline Alkaloids in Different Organs of Persian Poppy during Ontogenetical Stages

Abstract

Papaver bracteatum, a perennial species, has been known as a rich source of thebaine and a potential alternative to Papaver somniferum for the production of codeine and some semisynthetic antagonist drugs. In this study, ion mobility spectrum (IMS) of the root, leaf, bottom part of stem, upper part of stem, capsule wall, petal, and capsule content during developmental stages of P. bracteatum including annual rosette, perennial rosette, bud initiation, pendulous bud, preflowering, and lancing were investigated. The IMS revealed thebaine, papaverine, and noscapine as the major components of the extracted alkaloids. Based on the results of the study it appears that, at least in part, there is a competition among the biosynthesis pathways of papaverine, noscapine, and morphinan alkaloids from a common source. Root and capsule wall were the most potent organs for extraction of thebaine, while lancing stage was the best developmental stage for thebaine exploitation. However, it seems that total biomass of root and capsule wall plays a key role in the final selection of favorite organ. Although papaverine and noscapine in the stem at preflowering stage had the most quantity, significant amounts were found in the capsule wall. In general, total alkaloid content of leaf was lower than the other plant parts.

Phytochemical investigation among different populations of Papaver bracteatum Lindl. using ultrasonic - assisted extraction method followed by HPLC analysis

“…They introduced Persian poppy as a rich source for thebaine. Root and capsule were considered as the most potent organs for extraction of thebaine, also lancing was introduced as the best developmental stage [26]. In another study, they studied the developmental accumulation of thebaine and some gene transcripts in different organs of this plant [23].…”

“…The thebaine content of samples can change at different developmental stages [23,26]. So, it was evaluated during two developmental stages including pre-flowering (when the terminal of stem loses its plasticity and flower buds become erect) and lancing (at which the petals fall and the ripe capsule becomes evident).…”

“…20, No. 80: [23][24][25][26][27][28][29][30][31][32][33] medicines are so popular and they have been used in the industry [2].…”

Promising approaches for simultaneous enhancement of medicinally significant benzylisoquinoline alkaloids in opium poppy

“…If dropping out sanguinarine, the biosynthesis of BIA is strictly associated with differentiation and the development of specific cell types ( Facchini and Bird, 1998 ; Rezaei et al., 2016a ; Rezaei et al., 2016b ). Thus, with the exception of sanguinarine, cell suspension culture fails to produce BIAs as they lack differentiation.…”