Measurement of somatostatin release in rat brain by microdialysis

Takahashi, Hideo; Shintani, Yoshihiro; Okauchi, Takashi; Ishikawa, Masatoshi; Bando, Hiroshi; Azekawa, Takaharu; Morita, Yusuke; Saito, Shiro

doi:10.1016/0165-0270(94)90052-3

Cited by 10 publications

(3 citation statements)

References 25 publications

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“…An off-line sampling method with ultrafiltration (by vacuum sampling) for three proteins with molecular weights up to 68 kDa both in vivo and in vitro was described by Schneiderheinze et al [4]. [5] and by Asai et. al.…”

Section: Introductionmentioning

confidence: 99%

Development of a push-pull microdialysis sampling technique for the quantitative determination of proteins

et al. 2000

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An on-line push-pull sampling technique has been developed for continuous analysis of proteins of molecularweight from 5.7 to 67 kDa. The characteristics of the system include gradient elution with a total cycle time of 21 min, membrane stabihb,, unattended automatic operation, and adjustment of the sampling mode and extraction fraction (the ratio of the concentration of analyte in the clialysate to that in the sample) by varying the effective dialysis length. The push and pull flow rates were adjusted in a mannerwhich enabled three different modes of operation. When push-pull microclialysis was compared with conventional microclialysis sampling, significantly higher extraction fractions were obtained for all five model proteins studied. The technique has been applied to the quantification of proteins in cell samples. On-line fractionation enabled complementary MS identification of the proteins present.

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Section: Introductionmentioning

confidence: 99%

Development of a push-pull microdialysis sampling technique for the quantitative determination of proteins

et al. 2000

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“…of pentobarbital sodium. With a stereotaxic device (David Kopf Instruments, USA), as previously described [6], a guide cannula (22-gauge, 10 mm in length, CMA Microdialysis, Sweden) was implanted with its tip just dorsal to the anterior pituitary gland by using specific coordinates (0.9 mm lateral to the midline, 6.0 mm posterior to the bregma and 9.0 mm ventral to the dura), and fixed in position with dental resin (GC Reparisin, GC Shika, Kyoto). A 22-gauge stainless steel obturator was inserted into the end of the guide cannula until the start of the microdialysis experiment.…”

Section: Surgery For Microdialysismentioning

confidence: 99%

“…Tubes made of fluoroethylenepropylene (FEP) were connected between the syringe and probe and between the probe and the sampling tube. Perfusion was carried out by push methods [6], at a rate of 2 ,ul/min. The relative recovery rates for TRH and SRIF were 11.9 ± 3.0% (unpublished data) and 10.8 ± 0.6% [6], respectively.…”

Section: Surgery For Blood Samplingmentioning

confidence: 99%

Effects of Thyroidectomy on Thyrotropin-Releasing Hormone (TRH) and Somatotropin Release-lnhibiting Factor (SRIF) Patterns in Intrahypophysial Microdialysates in Rats.

et al. 1996

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Abstract.The effects of thyroidectomy on patterns of TRH and somatotropin release-inhibiting factor (SRIF) release from the hypothalamus were investigated by using a microdialysis technique. Thyroidectomized and sham-operated rats underwent placement of a guide cannula into the anterior pituitary gland to obtain dialysates, or implantation of an intravenous cannula into the right atrium for blood sampling.Seven days postoperatively dialysates were collected at a flow rate of 2 p1/min every 1 h. TRH concentrations in dialysates from thyroidectomized rats (0.43 ± 0.22 pg/h) were significantly higher than those from control rats (0.17 ± 0.02 pg/h).In contrast, SRIF concentrations in dialysates from thyroidectomized rats (2.45 ± 0.05 pg/h) were significantly lower than those from control rats (3.80 ± 0.22 pg/h).In addition, plasma TSH concentrations in thyroidectomized rats (24.8 ± 0.5 ng/ml) were increased compared with those in control rats (2.5 ± 0.1 ng/ml), and plasma GH concentrations were decreased from 68.6 ± 6.4 ng/ml in control rats to 21.2 ± 0.6 ng/ml in thyroidectomized rats. These findings indicate that TRH and SRIF releases from the hypothalamus are detectable by microdialysis method, and directly show the increase in TRH secretion and the decrease in SRIF secretion from hypothalamus in the hypothyroid state.

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