Measurement of Small Quantities of Insulin-Like Activity Using Rat Adipose Tissue. I. A Proposed Procedure*

Renold, Albert E.; Martin, Donald B.; Dagenais, Yves M.; Steinke, Jürgen; Nickerson, Rita J.; Sheps, Mindel C.

doi:10.1172/jci104168

Cited by 257 publications

(43 citation statements)

References 36 publications

(26 reference statements)

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“…Responses to tolbutamide4 (1 g in 20 ml distilled water administered intravenously over a 2-minute period to adults, 25 mg per kg of body weight to children) were assayed in terms of changes in blood sugar concentration as described by Unger and Madison (18), and in insulin-like activity (ILA) by a modification of the rat epididymal fat pad assay (19). Blood samples were drawn at 20, 30, 60, and 90 minutes.…”

Section: Methodsmentioning

confidence: 99%

Carbohydrate Metabolism in Two Forms of Hyperglyceridemia *

Knittle¹,

Ahrens²

1964

J. Clin. Invest.

127

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Section: Methodsmentioning

confidence: 99%

Carbohydrate Metabolism in Two Forms of Hyperglyceridemia *

Knittle¹,

Ahrens²

1964

J. Clin. Invest.

127

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“…Blood glucose was determined by the Hoffman method [16], modified for the autoanalyzer; ILA by the fat pad method of Renold et al [31], modified as described in a previous paper [24]; IRI by the Hales and Randle double antibody immunoassay [14].…”

Section: Intravenous Glucose Tolerance Test (I Vgtt)mentioning

confidence: 99%

Insulin levels in thyrotoxicosis and primary myxoedema: Response to intravenous glucose and glucagon

et al. 1970

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Summary. Glucose disappearance, insulin-like activity (ILA) and serum immunoreaetive insulin (Ii%I) were studied after intravenous injection of glucose or glueagon in patients suffering from thyrotoxieosis or primary myxoedema. A group of normal subjects was also investigated. Glucose disappearance rate appeared to be normal in hyperthyroid, and markedly reduced in hypothyroid subjects. Fasting ILA levels were significantly higher than normal in myxoedema, and lower than normal in thyrotoxicosis. On the other hand, fasting I1:r levels were higher than normal in myxoedema but normal in thyrotoxieosis. After glucose administration I LA in myxoedema remained at higher values than in thyrotoxieosis at any time of the study; in myxoedema, peak ILA levels were reached later than in thyrotoxieosis ; peak I1%1 levels of similar magnitude were reached slightly earlier than normal in thyrotoxicosis, and later in myxoedema; in the latter condition, elevated IgI levels were observed for a longer period than in either euthyroidism or hyperthyroidism. During this test the insulingogenic index showed only slight changes in normal and in thyrotoxie subjects, whereas it increased significantly up to 60 rain in myxoedema. The data indicate that the reduced glucose utilization in hypothyroidism is not due to insulin deficiency, but rather to insulin resistance. It also seems possible that thyroid function influences the time of maximal insulin response to intravenous glucose administration. After glueagon injection, the increase in blood glucose was lower and shorter than normal in thyrotoxieosis; it was initially somewhat slower, but later higher and more sustained than normM, in myxoedema. The smaller increment in blood glucose in the former condition is probably eormeeted with reduced glycogen stores. ILA response was very high and well sustained in myxoedema, whereas it was moderate and shortlasting in thyrotoxieosis. Ii%I response was higher than normal in hypothyroid subjects, and lower than normal in thyrotoxie subjects. No difference in the time required for maximal response was observed among the three groups.

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“…Serum and other biological fluids are known to act on several metabolic processes of muscle and adipose tissue in vitro in a manner comparable to crystalline insulin (1)(2)(3)(4)(5). Insulin-like activity of plasma (ILA) is only partially suppressed by anti-insulin serum, whereas the biological activity of crystalline insulin is completely neutralized (6,7).…”

Section: Introductionmentioning

confidence: 99%