Measurement of serum concentrations of inhibin‐A (α‐β<sub>A</sub> dimer) during human pregnancy

Muttukrishna, Shanthi; George, Lynne M.; Pa, Fowler; Groome, NP; Knight, P. G.

doi:10.1111/j.1365-2265.1995.tb02648.x

Cited by 112 publications

(67 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Hence, inhibin B levels decrease (2, 3). Inhibin A and E 2 levels increase after the dominant follicle selection (12,22,34). Inhibin B serum concentrations in the natural cycle showed a biphasic pattern in the mid to late follicular phase.…”

Section: Discussionmentioning

confidence: 94%

Relationship between inhibin A and B, estradiol and follicle growth dynamics during ovarian stimulation in normo-ovulatory women

Laven¹,

Jong²,

Fauser³

2005

eur j endocrinol

View full text Add to dashboard Cite

Objective: To investigate the relationship between serum concentrations of inhibin A, inhibin B and estradiol (E 2 ) and the number of developing follicles during the administration of exogenous follicle-stimulating hormone (FSH) in various regimens in normo-ovulatory volunteers and to evaluate if inhibins act as suitable markers for the number of developing follicles during ovarian stimulation. Design and methods: Serial hormone determinations and assessment of follicle numbers were carried out during unstimulated cycles and during various interventions with exogenous FSH. Subjects were randomized for FSH administration into the following groups: a single high dose (375 IU) during the early follicular phase (group A), 5 consecutive low doses (75 IU/day) starting in the mid follicular phase (group B) or daily low doses (75 IU/day) during the early to late follicular phase (starting on cycle days 3, 5 or 7; groups C, D and E respectively). Results: Extending the FSH window increases the number of small antral follicles and hence inhibin B serum concentrations. If such an intervention results in multi-follicular growth, mid follicular phase inhibin B (P ¼ 0.001) as well as late follicular phase inhibin B and inhibin A levels are significantly (P , 0.05 and P , 0.01 respectively) increased compared with mono-follicular cycles or the natural cycle. Although mid follicular inhibin B levels correlated well with the number of small antral (P , 0.05) and pre-ovulatory (P , 0.001) follicles in the late follicular phase, mid follicular inhibin A and estradiol serum concentrations only correlated with the number of pre-ovulatory follicles (P , 0.001 and P , 0.01 respectively). Conclusions: The present data extend our understanding of the relationship between follicle dynamics, serum inhibins and FSH during ovarian hyperstimulation. However, although mid follicular inhibin B does correlate with the number of developing follicles, it does not facilitate the identification of women at risk for multiple follicle development.European Journal of Endocrinology 152 395-401

show abstract

Section: Discussionmentioning

confidence: 94%

Relationship between inhibin A and B, estradiol and follicle growth dynamics during ovarian stimulation in normo-ovulatory women

Laven¹,

Jong²,

Fauser³

2005

eur j endocrinol

View full text Add to dashboard Cite

show abstract

“…Concentrations of circulating dimeric inhibin A rise in early pregnancy, fall after 12 weeks of gestation, and remain low until 24 weeks. Thereafter, concentrations increase gradually but with a marked rise in the third trimester [15] . The association between inhibin A and preeclampsia was first reported by Muttukrishna et al [16] , who found that levels of this analyte are significantly higher in women with established preeclampsia in the third trimester that among normotensive women.…”

Section: Discussionmentioning

confidence: 99%

Early Detection of Preeclampsia Using Inhibin A and Other Second-Trimester Serum Markers

Ree¹,

Hahn²,

Chang³

et al. 2011

Fetal Diagn Ther

View full text Add to dashboard Cite

Objective: The purpose of this study was to determine whether second-trimester maternal serum markers including inhibin A are useful for the detection of preeclampsia. Methods: Between January 2005 and March 2009, we analyzed the data of 4,764 subjects who underwent second-trimester multiple-marker screening for Down syndrome. Serum samples were assayed at 15+0 to 20+6 weeks for maternal serum alpha-fetoprotein (MSAFP), human chorionic gonadotrophin (hCG), unconjugated estriol (uE₃) and inhibin A. We reviewed all medical records retrospectively, and assessed the relationships of several markers with preeclampsia using logistic regression analysis. Results: The study sample included 41 patients who developed preeclampsia and a control group consisting of the other 4,723 healthy subjects treated between January 2005 and March 2009. There were no significant differences in gestational ages at blood sampling, maternal weights, gravidity and parity between the two groups. However, the mean ages, Apgar scores, gestational age at delivery and neonatal weights were significantly different between the study group and the control group. The levels of markers in the study group were significantly increased compared to the control group, 1.76 ± 2.68 for inhibin A, 1.18 ± 0.69 for MSAFP, and 1.62 ± 1.18 for hCG, but uE₃ did not differ significantly between the two groups. The AUC of inhibin A was 0.715, but the AUC of a three-marker combination model (0.800) was even better. A mid-trimester inhibin A concentration of 1.5 MoM or greater had a sensitivity of 60% and a false-positive rate of 16% for the prediction of preeclampsia. Inhibin A was the best predictor of preeclampsia. Three other markers were reliable predictive markers of preeclampsia. Conclusions: Inhibin A and other second-trimester serum markers may be useful for early detection of preeclampsia. Inhibin A was in fact the most important predictable marker among the markers we surveyed. The results of this study support those of previous studies, and provide quantified data elucidating the occurrence of preeclampsia.

show abstract

“…The development of sensitive and highly speci®c ELISAs for potentially bioactive dimeric inhibin A, inhibin B, activin A and follistatin (18±21) has now enabled us to measure these hormones in circulation in the menstrual cycle (18±21) and normal pregnancy (1,2,4,6). We have also shown that at term, placental homogenates contain high concentrations of inhibin A (1) and activin A (2) and others have reported that term placental trophoblasts secrete dimeric activin A in vitro (22).…”

Section: Introductionmentioning

confidence: 92%

Effect of cytokines and growth factors on the secretion of inhibin A, activin A and follistatin by term placental villous trophoblasts in culture

Mohan

Asselin

Sargent

et al. 2001

European Journal of Endocrinology

View full text Add to dashboard Cite

Objective: Maternal serum inhibin A and activin A are higher in pre-eclampsia than in normal pregnancy. The placenta is a source of these proteins in pregnancy. The aim of this study was to investigate the effect of growth factors and proin¯ammatory cytokines that are raised in pre-eclampsia on the secretion of dimeric inhibin A, activin A and follistatin by villous cytotrophoblasts in culture. Design and methods: Villous cytotrophoblasts were prepared from term placentae and cultured in serumfree media. Cells were treated with increasing concentrations of tumour necrosis factor (TNF)-a, interleukin (IL)-1b, transforming growth factor (TGF)-b1, granulocyte and monocyte colonystimulating factor (GMCSF), inhibin A, activin A and follistatin for 2 days. Culture supernatants were assayed for human chorionic gonadotrophin (hCG), inhibin A, activin A and follistatin as appropriate. Experiments were repeated at least three times with each cytokine or growth factor and the data pooled. Results: Cytotrophoblasts syncytialise and spontaneously secrete hCG, inhibin A and activin A in culture. Follistatin levels were ,20 pg/ml in most experiments. Activin A secretion was increased in culture in a dose-dependent manner by IL-1b (,150%, P , 0X05Y TNF-a (,35%, P 0X02 and GMCSF (,100%, P , 0X01X hCG secretion was inhibited in a dose-dependent manner by TNF-a (50%, P , 0X05X Inhibin A was stimulated by IL-1b (,30%, P 0X05X Inhibin A, activin A, follistatin or TGF-b1 did not have a signi®cant effect on any measured parameters. Conclusions: These data show that in¯ammatory cytokines increase the secretion of activin A by trophoblasts in culture. The presence of very low levels, or no follistatin (,20 pg/ml) in the culture media suggests`free' activin A could have autocrine/paracrine effects on cytotrophoblasts. Inhibin A secretion was stimulated by IL-1b. However, absence of an effect by the other cytokines investigated on inhibin A in this study suggests that the mechanism(s) involved in increasing maternal circulating levels of inhibin A and activin A in pre-eclampsia are controlled differentially.

show abstract

Measurement of serum concentrations of inhibin‐A (α‐β_A dimer) during human pregnancy

Cited by 112 publications

References 33 publications

Relationship between inhibin A and B, estradiol and follicle growth dynamics during ovarian stimulation in normo-ovulatory women

Relationship between inhibin A and B, estradiol and follicle growth dynamics during ovarian stimulation in normo-ovulatory women

Early Detection of Preeclampsia Using Inhibin A and Other Second-Trimester Serum Markers

Effect of cytokines and growth factors on the secretion of inhibin A, activin A and follistatin by term placental villous trophoblasts in culture

Contact Info

Product

Resources

About

Measurement of serum concentrations of inhibin‐A (α‐βA dimer) during human pregnancy

Cited by 112 publications

References 33 publications

Relationship between inhibin A and B, estradiol and follicle growth dynamics during ovarian stimulation in normo-ovulatory women

Relationship between inhibin A and B, estradiol and follicle growth dynamics during ovarian stimulation in normo-ovulatory women

Early Detection of Preeclampsia Using Inhibin A and Other Second-Trimester Serum Markers

Effect of cytokines and growth factors on the secretion of inhibin A, activin A and follistatin by term placental villous trophoblasts in culture

Contact Info

Product

Resources

About

Measurement of serum concentrations of inhibin‐A (α‐β_A dimer) during human pregnancy