Measurement of Serum Alpha-Fetoprotein in Early Infancy: Utilization of Dried Blood Specimens

Mizejewski, Gerald J.; Carter, Thomas P.; Beblowski, D W; Bellisario, Ronald

doi:10.1203/00006450-198301000-00009

Cited by 18 publications

(11 citation statements)

References 28 publications

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“…In the pediatric setting, the interpretation of αFP is complicated by the wide range and rapid variation of the physiologic plasmatic concentration in the first year of life (31,32), by physiologically higher levels in the preterm baby (14), and by the constitutionally higher and slowly decreasing concentrations in BWS/HH (33). Also, in our determinations, BWS/ HH cases have constitutively higher levels of αFP, confirming what already observed on plasmatic determinations (33).…”

Section: Discussionsupporting

confidence: 85%

α-Fetoprotein assay on dried blood spot for hepatoblastoma screening in children with overgrowth-cancer predisposition syndromes

Mussa

Pagliardini

et al. 2014

Pediatr Res

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Background: Beckwith-Wiedemann syndrome (BWS) and hemihyperplasia (HH) are overgrowth conditions with predisposition to hepatoblastoma for which early diagnosis patients undergo cancer screening based on determination of the tumor marker α-fetoprotein (αFP). Repeated blood draws are a burden for patients with consequent compliance issues and poor adherence to surveillance protocol. We sought to analyze feasibility and reliability of αFP dosage using an analytical micromethod based on blood dried on filter paper (DBS). Methods: Overall 143 coupled αFP determinations on plasma and DBS collected simultaneously were performed, of which 31 were in patients with hepatoblastoma predisposition syndromes and 112 were in controls. The plasma αFP dosage method was adapted to DBS adsorbed on paper matrix for newborn screening. results: There was strong correlation between plasmatic and DBS αFP (r 2 = 0.999, P < 0.001). Cohen's k coefficient for correlation was 0.96 for diagnostic cut-off of 10 U/ml (P < 0.001), commonly employed in clinical practice. The measurements on plasma and DBS were highly overlapping and consistent. conclusion: The DBS method allowed to dose αFP reliably and consistently for the concentrations commonly employed in clinical settings for the screening of hepatoblastoma, opening new scenarios about conducting cancer screening in overgrowth syndromes.

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Section: Discussionsupporting

confidence: 85%

α-Fetoprotein assay on dried blood spot for hepatoblastoma screening in children with overgrowth-cancer predisposition syndromes

Mussa

Pagliardini

et al. 2014

Pediatr Res

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“…The ti/j of a-FP in normal newborns has been reported to be about 5 days for the first weeks of life [1,3,12] and a little longer for premature babies (7.7 days) [12], In this study we found similar results for the TH group, i.e. a median value for a-FP ti/, of 4.9 days.…”

Section: Discussionsupporting

confidence: 79%

α-Fetoprotein in Congenital Hypothyroidism

Mengreli

Sarafidou²,

Petmezaki³

et al. 1990

Neonatology

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α-Fetoprotein (α-FP) was measured in dried blood spots from normal, congenital hypothyroid (CH) and transient hyperthyrotropinemic (TH) newborns as well as in serum from CH and TH babies together with thyroxine, triiodothyronine and thyrotropin. The half-life of α-FP had a median value of 12 days in the CH cases and 4.9 days in the TH cases. α-FP was significantly higher in the CH group before treatment and showed a significant rise after discontinuation of thyroxine therapy. It would appear that thyroid hormones influence α-FP metabolism and that a hypothyroid environment results in increased α-FP levels.

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“…In a study on the umbilical cord of the newborn fetus, fetal sex did not significantly affect the AFP values (1 2). The sex of the infant also appeared to have no influence on AFP serum concentrations during the first week of life (13). On the contrary, AFP concentration was higher in the mature full term male infants than age-matched females (14).…”

mentioning

confidence: 92%

Fetal sex and maternal alpha‐fetoprotein concentration at late normal singleton pregnancies

Chen

And

Huang

1994

Acta Obstet Gynecol Scand

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The aim of this study is to investigate maternal and fetal clinical parameters on maternal serum alpha-fetoprotein (MAFP) levels at late pregnancies. The studied subjects were 36 to 40 weeks pregnant. The pregnancies were singletons without any medical or gynecologic disease. Delivery followed within three days after blood sampling. A total of 192 subjects were included. The MAFP levels were measured by radioimmunoassay. The results showed the average MAFP was 108.2 ng/ml (SE 4.8 ng/ml). Male-fetus-bearers had higher MAFP (mean 121.4, SE 8.2 ng/ml, n = 84) than female-fetus-bearers (mean 97.9, SE 5.43 ng/ml, n = 108) (p = 0.014). MAFP did not have significant correlation with fetal weight (p > 0.05) regardless of whether the fetus was a male (p > 0.05) or female (p > 0.05). By stepwise multiple regression, fetal sex was revealed to be the only factor that can affect MAFP (p = 0.0071). MAFP did not correlate with maternal age, gravidity, parity, maternal weight, total weight gain during pregnancy, gestational weeks, or fetal weight (all p > 0.05). Since fetal sex is the only factor that influences MAFP levels at uncomplicated late pregnancies, MAFP values should be interpreted with caution.

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Measurement of Serum Alpha-Fetoprotein in Early Infancy: Utilization of Dried Blood Specimens

Cited by 18 publications

References 28 publications

α-Fetoprotein assay on dried blood spot for hepatoblastoma screening in children with overgrowth-cancer predisposition syndromes

α-Fetoprotein assay on dried blood spot for hepatoblastoma screening in children with overgrowth-cancer predisposition syndromes

α-Fetoprotein in Congenital Hypothyroidism

Fetal sex and maternal alpha‐fetoprotein concentration at late normal singleton pregnancies

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