Measurement of renin and prorenin in cattle, hog and horse

Nielsen, Arne Høj; Gotfredsen, P.; Nielsen, Poul; Hyttel, Poul; Poulsen, Knud

doi:10.1016/0300-9629(91)90193-g

Cited by 16 publications

(19 citation statements)

References 17 publications

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“…This method was previously described in more detail for the measurement of renin in bovine plasma, ovarian follicular fluid, and tissues [19][20][21]. The assay was standardized against a hog renin standard (Medical Research Council, Holly Hill, London, UK), and the renin concentrations are given as Goldblatt units (GU) per liter.…”

Section: Measurement and Identification Of Reninmentioning

confidence: 99%

Autoradiographic Localization and Characterization of Angiotensin II Receptors in the Bovine Placenta and Fetal Membranes1

Schauser¹,

Nielsen²,

Winther³

et al. 1998

Biology of Reproduction

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Autoradiography and angiotensin (Ang) II receptor binding studies showed that all parts of the bovine placenta and fetal membranes contained high densities of Ang II receptors throughout gestation. The receptors were predominantly subtype 2 (AT2) receptors in the fetal and subtype 1 (AT1) receptors in the maternal compartment. In the allantoamnionic membrane, Ang II receptors were evenly distributed in the mesenchymal tissue, with the highest expression around the few arteries. In the intercotyledonary and cotyledonary allantochorionic membrane, AT2 receptors as well as the less-expressed AT1 receptors were located on mesenchymal cells, especially adjacent to the allantoic endoderm, trophoblast cell layer, and arteries. In the mesenchymal tissue of the placentome, Ang II receptors were mostly expressed at the main branches of the fetal villi of the cotyledons. In the maternal part of the placentome, mainly AT1 receptors but also low densities of AT2 receptors and non-AT1/non-AT2 Ang II binding sites were found close to the stalk and at the main branches of the maternal crypts. Autoradiography revealed no changes in the pattern of distribution of the Ang II receptors throughout gestation. It is suggested that Ang II has an effect on regulatory as well as growth processes in these tissues.

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Section: Measurement and Identification Of Reninmentioning

confidence: 99%

Autoradiographic Localization and Characterization of Angiotensin II Receptors in the Bovine Placenta and Fetal Membranes1

Schauser¹,

Nielsen²,

Winther³

et al. 1998

Biology of Reproduction

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“…The method has been described and validated in detail for bovine and porcine plasma and ovarian follicular fluid, recently (Nielsen et al 1991b;Hagemann et al 1992). The assay was standardized against a hog renin standard (MRC, Holly Hill, London, UK).…”

Section: Renin Determinationmentioning

confidence: 99%

“…Prorenin was activated by limited proteolysis with trypsin-TPCK (Worthington Biochemical, Freehold, NJ), freshly dissolved in 0.5 mol/L Tris-HC1 buffer (pH 7.5) containing 5 mmol/L calcium chloride and 2% human serum albumin (Statens Seruminstitut, Copenhagen, Denmark). In our routine procedure for porcine plasma (Nielsen et al 1991b) a 40 pL sample is incubated for 1 h at 0°C with 20 pL trypsin solution. The final trypsin concentration was 2 mg/mL (Nielsen et al 1991b).…”

Section: Activation Of Proreninmentioning

confidence: 99%

“…In our routine procedure for porcine plasma (Nielsen et al 1991b) a 40 pL sample is incubated for 1 h at 0°C with 20 pL trypsin solution. The final trypsin concentration was 2 mg/mL (Nielsen et al 1991b). The reaction was terminated by adding 20 pL soybean trypsin inhibitor (type I-S, Sigma), dissolved in 0.1 mol/L sodium chloride.…”

Section: Activation Of Proreninmentioning

confidence: 99%

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Renin and Prorenin in Reproductive Tissues During Gestation in Pigs and Cattle

Hagemann¹,

Dantzer²,

Nielsen³

et al. 1993

Clin Exp Pharma Physio

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1. High concentrations of prorenin and active renin were previously found in ovarian follicular fluid from cattle but not from pigs. In the present study female reproductive tissues and fluids from cattle and pigs during gestation were investigated to clarify a possible species difference in active renin and prorenin concentrations. 2. Very high concentrations of active renin but no prorenin were found in corpus luteum from both species. 3. Relatively low concentrations of active renin, in the same order as in maternal blood plasma, were found in myometrium, endometrium, placenta and fetal membranes from both species. Prorenin was undetectable in these tissues except for bovine myometrium and porcine endometrium in some animals. 4. The concentrations of active renin and prorenin in amnionic fluid from both species were below the maternal plasma values. In allantoic fluid the concentrations were higher than in amnionic fluid. 5. The plasma concentrations of active renin and prorenin did not change during gestation in pigs. This finding is in contrast to the observations in humans and does not support a systemic effect of prorenin during gestation. 6. The presence of renin in the reproductive tissues, especially the very high concentrations in the corpus luteum, indicates a local function of the renin-angiotensin system during gestation.

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“…7,[19][20][21][22][23][24][25] Given that endog- Error bars are present but extremely small. The study reported here involved a novel assay with which changes in plasma renin concentration relative to the clinical stresses associated with renal autograft ischemia-reperfusion injury in cats were detected.…”

Section: Discussionmentioning

confidence: 99%