Measurement of prothrombin time (PT) and activated partial thromboplastin time (APTT) on canine citrated plasma samples following different storage conditions

Rizzo, Francesca; Papasouliotis, Kostas; Crawford, EM; Dodkin, SJ; Cue, SM

doi:10.1016/j.rvsc.2007.10.001

Cited by 33 publications

(37 citation statements)

References 14 publications

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“…Previously, the effect of anticoagulant and storage conditions on platelet clumping has been evaluated in dogs [11] and the stability of canine plasma stored at room temperature or at 4℃ for hemostasis testing has been investigated at 24, 48, 72 and 96 h post sampling [6]. Additionally, the effect of long-term storage at -20℃ [16] and that of 6-month storage on hemostatic function testing [2] in the dog have been reported. The possible impact of 24-h storage on PT, aPTT and fibrinogen concentration, however, has rarely been assessed.…”

Section: Introductionmentioning

confidence: 99%

Effect of storage conditions on prothrombin time, activated partial thromboplastin time and fibrinogen concentration on canine plasma samples

et al. 2010

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The present study was to assess the effect of storage conditions on prothrombin time (PT), activated partial thromboplastin time (aPTT) and fibrinogen concentration in blood samples of healthy dogs. Thirty-five dogs of various breeds were included in the study. Citrated blood samples were obtained and plasma was divided into four aliquots to assess selected clotting parameters by means of a coagulometer. The first aliquot was analysed within 1 h after collection, while the remaining 3 were stored at 8℃ for 4, 8 and 24 h, respectively. One-way repeated measures analysis of variance documented a significant decreasing effect on PT at 24 h compared to 8 h and on fibrinogen concentration after 8 and 24 h compared to sampling time and at 4 and 24 h compared to 8 h post sampling. In conclusion, the results of this study indicate that only fibrinogen appears prone to significant decrease. In fact, aPTT is not substantially affected by refrigeration for at least 24 h post sampling and PT showed a statistical difference that does not necessary indicate biological significance as the results obtained were within reference intervals for the dog.

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Section: Introductionmentioning

confidence: 99%

Effect of storage conditions on prothrombin time, activated partial thromboplastin time and fibrinogen concentration on canine plasma samples

et al. 2010

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“…Citrated plasma and PT reagent (containing 200 μL of thromboplastin-D solution and 100 μL of the citrated plasma) was incubated for 1 min at 37°C. When the clot was observed, the PT time was recorded (11,12).…”

Section: Prothrombin Time (Pt)mentioning

confidence: 99%

“…After shaking the tube for about 20 seconds in Bainmarie, the tube was ejected from water and it was searched for the clot. The time required for clot formation was recorded (12).…”

Section: Activated Partial Thromboplastin Time (Aptt)mentioning

confidence: 99%

Properties of biological and biochemical effects of the Iranian saw-scaled viper (Echis carinatus) venom

Babaie¹,

Salmanizadeh²,

Zolfagharian³

et al. 2014

BLL

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Abstract:The venom of Echis carinatus is rich in proteins and peptides effective on the hemostatic system. This venom is contains metalloproteinase which convert prothrombin to meizothrombin. The prothrombin activator which leads to the formation of small blood clots inside the blood vessels throughout the body. To understand the mechanism of the effects of Iranian Echis carinatus venom, the effects of E. carinatus on human and Wistar rat plasma, plasma proteins (prothrombin and fi brinogen) and blood coagulation were studied. Proteolytic activity of the crude venom on blood coagulation factors such as prothrombin, partial thromboplastin and fi brinogen times were studied. In the present study the PT test for human plasma was reduced from 13.4 s (± 0.59) to 8.6 s (± 0.64) when human plasma was treated with crude venom (concentration of venom was 1 mg/ml) and for rat plasma PT was reduced from 14.5 s (± 0.47) to 8 s (± 0.49). Some possible biological and biochemical effects of IEc crude venom were investigated. The blood coagulation in human and in rat were investigated in vivo and in-vitro. In this paper, we show that the procoagulant action of Echis carinatus venom is due in part to a protein component that activates prothrombin and the procoagulant activity on human and rat plasma was evaluated (Tab. 2, Fig. 2, Ref. 31). Text in PDF www.elis.sk.

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“…Sonja et al used only normal samples while our data had normal and abnormal samples, and both were well preserved. Rizzo et al [4] stored plasma of ill dogs at À20°C up to 7 months and came to the conclusion that APTT value is not stable on storage. From these data, it seems that liquid nitrogen storage is better than deep freeze storage.…”

Section: Sirmentioning

confidence: 99%

Stability of citrated plasma samples in liquid nitrogen

Patel

Rathod

Chauhan

et al. 2013

Int J Lab Hematology

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Measurement of prothrombin time (PT) and activated partial thromboplastin time (APTT) on canine citrated plasma samples following different storage conditions

Cited by 33 publications

References 14 publications

Effect of storage conditions on prothrombin time, activated partial thromboplastin time and fibrinogen concentration on canine plasma samples

Effect of storage conditions on prothrombin time, activated partial thromboplastin time and fibrinogen concentration on canine plasma samples

Properties of biological and biochemical effects of the Iranian saw-scaled viper (Echis carinatus) venom

Stability of citrated plasma samples in liquid nitrogen

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