Measurement of Pneumococcal Polysaccharide Antibodies

Sorensen, Ricardo U.; Leiva, Lily E.

doi:10.1007/s10875-013-9977-z

Cited by 11 publications

(15 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A comparison of pneumococcal polysaccharide antibody response and S. typhi Vi vaccine response was recently conducted in 16 healthy volunteers, 22 common variable immunodeficiency patients, and 27 hypogammaglobulinemic patients and showed superiority of anti- S. typhi Vi vaccine responses to discriminate between groups. However, pneumococcal response was assessed by a commercial PPV-coated ELISA and these ELISAs, measuring a combined IgG response, have limited value to diagnose patients with SAD (13). Using the same commercial ELISA kit for S. typhi Vi vaccine, Sánchez-Ramón and colleagues found a minimum postvaccination IgG concentration of 32 and 7.4 U/ml and minimum fold increase of 3.4 and 1 in a healthy and Common Variable Immunodeficiency group, respectively, which shows that our proposed criteria discriminate healthy subjects from SAD subjects (16).…”

Section: Discussionmentioning

confidence: 99%

“…Since the introduction of the conjugated pneumococcal vaccine for routine immunization of children, in most industrialized countries, even more individuals have been primed with conjugated polysaccharides (11, 12). Therefore, the measurement of pneumococcal polysaccharide antibodies may no longer be the preferable assessment of the T-independent response (13). Other limitations include cost, workload, and concerns about hyporesponsiveness to polysaccharide vaccines with subsequent administrations (14).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Fifth Percentile Cutoff Values for Antipneumococcal Polysaccharide and Anti-Salmonella typhi Vi IgG Describe a Normal Polysaccharide Response

et al. 2017

View full text Add to dashboard Cite

BackgroundSerotype-specific antibody responses to unconjugated pneumococcal polysaccharide vaccine (PPV) evaluated by a World Health Organization (WHO)-standardized enzyme-linked immunosorbent assay (ELISA) are the gold standard for diagnosis of specific polysaccharide antibody deficiency (SAD). The American Academy of Allergy, Asthma and Immunology (AAAAI) has proposed guidelines to interpret the PPV response measured by ELISA, but these are based on limited evidence. Additionally, ELISA is costly and labor-intensive. Measurement of antibody response to Salmonella typhi (S. typhi) Vi vaccine and serum allohemagglutinins (AHA) have been suggested as alternatives. However, there are no large cohort studies and cutoff values are lacking.ObjectiveTo establish cutoff values for antipneumococcal polysaccharide antibody response, anti-S. typhi Vi antibody, and AHA.MethodsOne hundred healthy subjects (10–55 years) were vaccinated with PPV and S. typhi Vi vaccine. Blood samples were obtained prior to and 3–4 weeks after vaccination. Polysaccharide responses to 3 serotypes were measured by WHO ELISA and to 12 serotypes by an in-house bead-based multiplex assay. Anti-S. typhi Vi IgG were measured with a commercial ELISA kit. AHA were measured by agglutination method.ResultsApplying AAAAI criteria, 30% of healthy subjects had a SAD. Using serotype-specific fifth percentile (p5) cutoff values for postvaccination IgG and fold increase pre- over postvaccination, only 4% of subjects had SAD. One-sided 95% prediction intervals for anti-S. typhi Vi postvaccination IgG (≥11.2 U/ml) and fold increase (≥2) were established. Eight percent had a response to S. typhi Vi vaccine below these cutoffs. AHA titer p5 cutoffs were ½ for anti-B and ¼ for anti-A.ConclusionWe establish reference cutoff values for interpretation of PPV response measured by bead-based assay, cutoff values for S. typhi Vi vaccine responses, and normal values for AHA. For the first time, the intraindividual consistency of all three methods is studied in a large cohort.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fifth Percentile Cutoff Values for Antipneumococcal Polysaccharide and Anti-Salmonella typhi Vi IgG Describe a Normal Polysaccharide Response

et al. 2017

View full text Add to dashboard Cite

show abstract

“…13 The interpretation of an adequate or inadequate response to PPV23 has been recommended by experts in the field using this cutoff, 29 but strict definitions based on 1.3 mg/mL should not be used with multiplex results. 30 Results obtained with multiplex assays are not equivalent to ELISA and may poorly correlate. 31 The method a laboratory uses for measurement of pneumococcal antibody titers when interpreting results is important because most commercial laboratories use multiplex methods because they are high throughput.…”

Section: Vaccine Titer Responsesmentioning

confidence: 99%

Antibody deficiency testing for primary immunodeficiency

Marsh

2019

Annals of Allergy, Asthma & Immunology

View full text Add to dashboard Cite

A significant proportion of primary immunodeficiency diseases include humoral deficiency. Clinically available testing can be used to evaluate patients for suspected antibody deficiency disorders. General immunoglobulin levels screen for hypogammaglobulinemia. Antibody responses to infections or vaccinations can determine whether patients are able to mount specific antibody responses; protein and polysaccharide vaccines allow evaluation of the capability to mount T-celledependent and T-celleindependent responses, respectively. Antibody responses to neoantigens, such as the inactivated Salmonella Typhi vaccine or rabies vaccine, can allow physicians to evaluate specific antibody responses in patients who are receiving immunoglobulin replacement therapies. B-cell enumeration and phenotyping can be used to screen patients for absent or low B-cell counts or absent or low class-switched memory B-cell populations.

show abstract

“…Clinical validation of the usefulness of Luminex is still in progress. 18,19 PPV23 has the advantage that a high number of serotypes are included, disallowing a diagnosis based on a single antigen response. Measurement of total antipneumococcal IgG in a single test that produces 1 numeric value without differentiating specific antibodies to individual serotypes is not useful.…”

Section: Specific Antibody Deficienciesmentioning

confidence: 99%