Measurement of plasma protein and lipoprotein binding of pyrethroids

Sethi, Pankaj K.; Muralidhara, Srinivasa; Bruckner, James V.; White, Catherine A.

doi:10.1016/j.vascn.2014.06.002

Cited by 26 publications

(10 citation statements)

References 30 publications

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“…The unbound fraction of the drug was approximately fourfold higher in neonates than adults, but progressively decreased, reaching adult levels in the 3-to 6-y-old group. Sethi et al (18) previously confirmed that cyclosporine binds primarily to the lipoprotein fraction of adult human plasma. The lipoprotein levels in plasma samples were not quantified, as others have characterized the ontogeny of major lipoproteins.…”

Section: Discussionmentioning

confidence: 85%

Ontogeny of plasma proteins, albumin and binding of diazepam, cyclosporine, and deltamethrin

et al. 2015

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Section: Discussionmentioning

confidence: 85%

Ontogeny of plasma proteins, albumin and binding of diazepam, cyclosporine, and deltamethrin

et al. 2015

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“…Poor solubility of pyrethroids in aqueous buffers that serve as a mobile phase coupled with adherence or nonspecific binding to glass, polymers, metals, and filter membranes proved problematic. To minimize such difficulties, Sethi et al (2014) developed a serial solvent extraction technique, described subsequently. Lobind plastic pipette tips (Eppendorf, Hamburg, Germany) were used to avoid adherence.…”

Section: Methodsmentioning

confidence: 99%

“…Binding of DLM, CIS, and TRANS to rat and human plasma components was quantified by the three-step organic solvent extraction method developed by Sethi et al (2014). Stock solutions of each unlabeled pyrethroid were prepared with DMSO.…”

Section: Methodsmentioning

confidence: 99%

Plasma Protein and Lipoprotein Binding ofCis- andTrans-Permethrin and Deltamethrin in Adult Humans and Rats

Sethi¹,

Bruckner²,

Mortuza³

et al. 2019

Drug Metab Dispos

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The majority of residents of the United States, Canada, and Europe are exposed to pyrethroids, the most commonly used class of insecticides. Surprisingly little is known about key aspects of their pharmacokinetics, including their mode of transport in the systemic circulation. This study tested the hypothesis that pyrethroids are transported by both plasma lipoproteins and proteins, similarly to other highly lipophilic environmental contaminants. Other aims were to characterize the binding of representative type I and II pyrethroids, and to compare their binding to rat versus human plasma. Binding of 14 C-labeled cis-permethrin (CIS), trans-permethrin (TRANS) and deltamethrin (DLM) to proteins and lipoproteins was measured by sequential extraction of spiked plasma with isooctane, 2-octanol, and acetonitrile. Binding of DLM, CIS, and TRANS to plasma proteins and lipoproteins was linear from 250 to 750 nM; concentrations present in the plasma of orally dosed rats. Binding of DLM to high-density lipoprotein was twice that to low-density lipoprotein. Binding of DLM, CIS, and TRANS was ∼2-fold greater to proteins than to lipoproteins of rat and human plasma; albumin was primarily responsible for protein binding. Higher total binding of each pyrethroid to human (∼90%) than to rat (∼80%) plasma resulted from higher protein binding in human plasma. This was attributable in part to the higher albumin/protein content of human plasma. Rat albumin exhibited lower pyrethroid binding capacity than did human albumin. The results of this investigation indicate that albumin and lipoproteins play a major role in binding and transport of pyrethroids in the systemic circulation of both rats and humans.

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“…Due to their high insecticidal activity, comparatively lower mammalian toxicity and rapid degradation in the environment, they have generally replaced other classes of insecticides (organochlorines, organophosphates and carbamates). They are widely used in insect sprays, wood preservation, lice treatment, veterinary medicine, mosquito control, and against malaria . However, the increasing production and application of pyrethroids has led to frequent human exposures by inhalation, skin absorption and ingestion via entry into the food chain and the ecosystem …”

Section: Introductionmentioning

confidence: 99%

Gas chromatography/negative chemical ionization mass spectrometry of transfluthrin in rat plasma and brain

Hooshfar

Mortuza

Rogers

et al. 2017

Rapid Comm Mass Spectrometry

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Measurement of plasma protein and lipoprotein binding of pyrethroids

Cited by 26 publications

References 30 publications

Ontogeny of plasma proteins, albumin and binding of diazepam, cyclosporine, and deltamethrin

Ontogeny of plasma proteins, albumin and binding of diazepam, cyclosporine, and deltamethrin

Plasma Protein and Lipoprotein Binding ofCis- andTrans-Permethrin and Deltamethrin in Adult Humans and Rats

Gas chromatography/negative chemical ionization mass spectrometry of transfluthrin in rat plasma and brain

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