Measurement of plasma chromogranin A concentrations for assessment of stress responses in dogs with insulin-induced hypoglycemia

Akiyoshi, Hideo; Aoki, Mica; Shimada, Terumasa; Noda, Katsura; Kumagai, D.; Saleh, Nahed; Sugii, Shunji; Ohashi, Fumihito

doi:10.2460/ajvr.2005.66.1830

Cited by 22 publications

(23 citation statements)

References 33 publications

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“…However, the CgA levels were elevated only in patients with renal colic and did not reach statistical significance. In the past literature, the correlation between the severity of pain or stress and CgA levels is controversial [27][28][29][30][31][32]. In our study, there was no statistically significant correlation between the CgA levels and VAS scores in the patient groups.…”

Section: Discussioncontrasting

confidence: 76%

Pain levels associated with renal colic and primary dysmenorrhea: a prospective controlled study with objective and subjective outcomes

Ayan

Söğüt

Taş

et al. 2012

Arch Gynecol Obstet

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Section: Discussioncontrasting

confidence: 76%

Pain levels associated with renal colic and primary dysmenorrhea: a prospective controlled study with objective and subjective outcomes

Ayan

Söğüt

Taş

et al. 2012

Arch Gynecol Obstet

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“…The stress response system includes the sympathoadrenalmedullary (SAM) system and the hypothalamus-pituitaryadrenal (HPA) axis (Akiyoshi et al, 2005a). The activities of the SAM system and the HPA axis can be biochemically evaluated by measuring catecholamines and cortisol, respectively (Lee et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

“…Although the physiological role of CgA is still under investigation, the accumulated evidence provides convincing support for a role of CgA in counteracting sympathetic activity (Gallina et al, 2011). In veterinary medicine, the measurement of canine plasma CgA concentrations has also been proposed as a useful index for evaluation of an acute -E-mail: jjceron@um.es stress response (Akiyoshi et al, 2005a). In addition, salivary CgA has been indicated as a possible marker of stress response in cows (Ninomiya and Sato, 2011), although its physiological role in saliva and in the stress response is still debated.…”

Section: Introductionmentioning

confidence: 99%

Measurement of chromogranin A in porcine saliva: validation of a time-resolved immunofluorometric assay and evaluation of its application as a marker of acute stress

Escribano

Soler

Gutiérrez

et al. 2013

Animal

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The objective of this study was to develop and validate a time-resolved immunofluorometric assay (TR-IFMA) for porcine salivary chromogranin A (CgA) measurements, using a species-specific antibody, and evaluate its behaviour in an acute stress model. Polyclonal antibodies were produced in rabbits immunized with a synthetic porcine fragment of CgA 3592379 and used to develop a sandwich TR-IFMA. This TR-IFMA was analytically validated and showed intra-and inter-assay coefficients of variation of 6.23% and 5.82%, respectively, an analytical limit of detection of 4.27 3 10 23 mg/ml and a limit of quantification of 24.5 3 10 23 mg/ml. The assay also demonstrated a high level of accuracy, as determined by linearity under dilution (r 5 0.975) and recovery tests. When a model of experimental acute stress, in which animals were immobilized for 3 min with a nose snare (stressor stimulus), was applied, a significant increase (P , 0.05) in CgA levels in saliva was detected at 15 min post-stressor stimulus. These results indicate that the assay developed in this study could measure CgA in porcine saliva in a reliable way and that the concentrations of CgA in saliva samples of pigs increase after an acute stress situation.

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“…In addition, CgA has been detected in salivary glands of humans and various animal species [72–74]. The release of CgA in saliva is mediated by the secretion of catecholamines [75], and has been related to the activation of SAM [76]. In humans, the measurement of CgA in saliva has been used as a reliable marker of SAM activation as an alternative to adrenaline and noradrenaline [77], which have high variability in results and low stability in saliva [78–80].…”

Section: Biomarkers Of Stress Studied In Pigsmentioning

confidence: 99%

Causes, consequences and biomarkers of stress in swine: an update

et al. 2016

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BackgroundIn recent decades there has been a growing concern about animal stress on intensive pig farms due to the undesirable consequences that stress produces in the normal physiology of pigs and its effects on their welfare and general productive performance. This review analyses the most important types of stress (social, environmental, metabolic, immunological and due to human handling), and their biological consequences for pigs. The physio-pathological changes associated with stress are described, as well as the negative effects of stress on pig production. In addition an update of the different biomarkers used for the evaluation of stress is provided. These biomarkers can be classified into four groups according to the physiological system or axis evaluated: sympathetic nervous system, hypothalamic-pituitary-adrenal axis, hypothalamic-pituitary-gonadal axis and immune system.ConclusionsStress it is a process with multifactorial causes and produces an organic response that generates negative effects on animal health and production. Ideally, a panel of various biomarkers should be used to assess and evaluate the stress resulting from diverse causes and the different physiological systems involved in the stress response. We hope that this review will increase the understanding of the stress process, contribute to a better control and reduction of potential stressful stimuli in pigs and, finally, encourage future studies and developments to better monitor, detect and manage stress on pig farms.

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Measurement of plasma chromogranin A concentrations for assessment of stress responses in dogs with insulin-induced hypoglycemia

Cited by 22 publications

References 33 publications

Pain levels associated with renal colic and primary dysmenorrhea: a prospective controlled study with objective and subjective outcomes

Pain levels associated with renal colic and primary dysmenorrhea: a prospective controlled study with objective and subjective outcomes

Measurement of chromogranin A in porcine saliva: validation of a time-resolved immunofluorometric assay and evaluation of its application as a marker of acute stress

Causes, consequences and biomarkers of stress in swine: an update

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