Measurement of plasma acetate kinetics using high-performance liquid chromatography

Persson, Mai; Bleiberg, B.; Kiss, Dorottya; Miles, John M.

doi:10.1016/0003-2697(91)90520-4

Cited by 15 publications

(8 citation statements)

References 16 publications

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“…As the level of formate in case 1 was six times higher than that in case 2, measuring formate in addition to methanol is considered to be useful in suspected methanol poisoning in order to elucidate the solution ingested. Acetate concentrations in both cases were within a control range (0.06 mM [34], 0.03 mM [35], 0.181 mM [32]). Therefore, formate poisoning was verified.…”

Section: Practical Applicationsmentioning

confidence: 84%

Simultaneous determination of formate and acetate in whole blood and urine from humans using gas chromatography–mass spectrometry

Kage¹,

Kudo

Ikeda

et al. 2004

Journal of Chromatography B

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Section: Practical Applicationsmentioning

confidence: 84%

Simultaneous determination of formate and acetate in whole blood and urine from humans using gas chromatography–mass spectrometry

Kage¹,

Kudo

Ikeda

et al. 2004

Journal of Chromatography B

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“…p-Bromophenacyl ester derivatives of acetate and NAA were prepared using p-bromophenacylbromide and crown ether (a phase transfer catalyst) in acetonitrile (Durst et al, 1975;Persson et al, 1991). Briefly, 100 l of acetonitrile and 40 l of p-bromophenacylbromide reagent were added to the lyophilized tissue extract prepared as above in a microcentrifuge tube (each vial containing 200-l equivalent of the 20% tissue homogenate), and the vials were tightly capped.…”

Section: Methodsmentioning

confidence: 99%

Progress toward Acetate Supplementation Therapy for Canavan Disease: Glyceryl Triacetate Administration Increases Acetate, but NotN-Acetylaspartate, Levels in Brain

Mathew¹,

Arun²,

Madhavarao³

et al. 2005

J Pharmacol Exp Ther

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Canavan disease (CD) is a fatal genetic neurodegenerative disorder caused by mutations in the gene for aspartoacylase, an enzyme that hydrolyzes N-acetylaspartate (NAA) into L-aspartate and acetate. Because aspartoacylase is localized in oligodendrocytes, and NAA-derived acetate is incorporated into myelin lipids, we hypothesize that an acetate deficiency in oligodendrocytes is responsible for the pathology in CD, and we propose acetate supplementation as a possible therapy. In our preclinical efforts toward this goal, we studied the effectiveness of orally administered glyceryl triacetate (GTA) and calcium acetate for increasing acetate levels in the murine brain. The concentrations of brain acetate and NAA were determined simultaneously after intragastric administration of GTA. We found that the acetate levels in brain were increased in a dose-and time-dependent manner, with a 17-fold increase observed at 1 to 2 h in 20-to 21-day-old mice at a dose of 5.8 g/kg GTA. NAA levels in the brain were not significantly increased under these conditions. Studies using mice at varying stages of development showed that the dose of GTA required to maintain similarly elevated acetate levels in the brain increased with age. Also, GTA was significantly more effective as an acetate source than calcium acetate. Chronic administration of GTA up to 25 days of age did not result in any overt pathology in the mice. Based on these results and the current Food and Drug Administration-approved use of GTA as a food additive, we propose that it is a potential candidate for use in acetate supplementation therapy for CD.

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“…To ensure fi-hydroxybutyrate has high UV absorption and enhanced liquid chromatographic characteristics, derivatization is applicable. A commercial reagent, bromoacetophenone, has been used to derivatize plasma acetate before HPLC analysis [16], but a very complicated extraction step including ether extraction, centrifugafion, and drying is required before derivafizafion, procedures which usually took approximately 5h. After this pretreatment derivatization of the residue with bromoacetophenone was also tedious and…”

Section: Introductionmentioning

confidence: 99%

“…Colorimetric [11], gas chromatographic (GC) [12][13][14][15], high-performance liquid chromatographic (HPLC) [16], and nuclear magnetic resonance spectroscopic [17,18] techniques have been introduced for measurement of fi-hydroxybutyrate concentrations. Head-space GC is the most widely used technique for analysis of fi-hydroxybutyrate in a variety of matrices.…”

Section: Introductionmentioning

confidence: 99%

Trace analysis of β-hydroxybutyrate in human plasma by derivatization and high-performance liquid chromatography

Hsu

Chen

2003

Chromatographia

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KeyWordsColumn liquid chromatography DerTvatizatTon fi-Hydroxybutyrate in plasma SummaryA sTmple and sensitTve liquid chromatographTc method is described for determTnation of fi-hydroxybutyrate, as a highly sensitive derivative, from human plasma. Plasma spiked wth/F hy droxybutyrate was extracted with acetonitrile and derivatized with 4 bromomethyl / methox ycoumarin, in a homogeneous system, using ]_5 crown 5 as activator. The resulting derivative was separated on a LiChroCART Rp C18 column wth 40% methanol in water as mobile phase and 2,2' dinitrobiphenyl as internal standard. Conditions affecting the extraction and deriva tization of/F hydrox,/butyrate from spiked plasma were investigated. For/F hydroxybutyrate de termTnatTon the detection limit (S/N-3; sample sTze, 10 M,L) was approxTmately 5 nmol mL 1; the relatTve standard deviatTon was <9% for Tntra-day assay (n-6) and < 10% for Tnter-day assay (n -6).All recoveries were > 98%.

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Measurement of plasma acetate kinetics using high-performance liquid chromatography

Cited by 15 publications

References 16 publications

Simultaneous determination of formate and acetate in whole blood and urine from humans using gas chromatography–mass spectrometry

Simultaneous determination of formate and acetate in whole blood and urine from humans using gas chromatography–mass spectrometry

Progress toward Acetate Supplementation Therapy for Canavan Disease: Glyceryl Triacetate Administration Increases Acetate, but NotN-Acetylaspartate, Levels in Brain

Trace analysis of β-hydroxybutyrate in human plasma by derivatization and high-performance liquid chromatography

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