Measurement of Placental Perfusion by Dynamic Contrast-Enhanced MRI at 4.7 T

Alison, Marianne; Quibel, T.; Balvay, D.; Autret, Gwennhaël; Bourillon, Camille; Chalouhi, G. E.; Deloison, B.; Salomon, Laurent; Cuenod, Charles‐André; Clémеnt, Olivier; Siauve, Nathalie

doi:10.1097/rli.0b013e3182856a25

Cited by 23 publications

(25 citation statements)

References 58 publications

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“…F was 149 mL/min/100 mL for the whole placenta; it was higher in the fetal layer (301.6 ± 188.73 mL/min/100 mL), and lower in the maternal layer (124 ± 95.6 mL/min/100 mL), consistent with previous reports [8]. Vb was 46.7 ± 9.0 for the placenta as a whole, 50 ± 9.7 in the fetal layer, and 42.9 ± 9.0 in the maternal layer.…”

Section: Resultssupporting

confidence: 82%

“…Our results for the control group were similar to those reported in the literature under normal conditions, with a mean placental blood flow of 140–149 mL/min/100 mL, in accordance with previous results obtained with DCE MRI [5-8, 13]. The fractional blood volume (46.7%) was also similar to those found in the literature [5-8, 13]. Our animal model was effective, with significantly lower fetal and placental weights for the L-NAME group than for controls.…”

Section: Discussionsupporting

confidence: 82%

“…DCE MRI was chosen to study placental function in this model: it is a robust method for assessing placental perfusion, and has been used successfully in several studies [5, 8, 12, 19-23]. Our results for the control group were similar to those reported in the literature under normal conditions, with a mean placental blood flow of 140–149 mL/min/100 mL, in accordance with previous results obtained with DCE MRI [5-8, 13].…”

Section: Discussionmentioning

confidence: 99%

“…All ROIs were defined manually: one over the aorta (AIF) and three over each placenta (one over the whole placenta, one on the “fetal side,” and one on the “maternal side”). The latter two were determined by k-mean classification differentiating their enhancement pattern [8]. ROIs were placed manually within one of the images and then propagated automatically to all other images using PhysioD3D software.…”

Section: Methodsmentioning

confidence: 99%

“…Placental blood flow and maternal vascular placental fractional volume have been determined in mice and rats under physiological conditions, and permeability coefficients in mice have been published [5, 6, 8]. It has also been reported that in cases of FGR, there are modifications of placental blood flow and maternal vascular placental fractional volume [7].…”

Section: Introductionmentioning

confidence: 99%

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Assessment of Placental Perfusion in the Preeclampsia L-NAME Rat Model with High-Field Dynamic Contrast-Enhanced MRI

et al. 2018

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Purpose: To evaluate placental function and perfusion in a rat model of preeclampsia infused with L-nitro-arginine methyl ester (L-NAME) by dynamic contrast-enhanced (DCE) MRI using gadolinium chelates. Methods: Pregnant female Sprague-Dawley rats were fitted on embryonic day 16 (E16) with subcutaneous osmotic minipumps loaded to deliver, continuously, L-NAME (50 mg/day per rat; case group) or saline solution (control group). DCE MRI was performed on E19 using gadolinium chelates and a 4.7-T MRI apparatus for small animals. Quantitative analysis was performed using an image software program: placental blood flow (perfusion in mL/min/100 mL of placenta) and fractional volume of the maternal vascular placental compartment (ratio between the placental blood volume and the placental volume, Vb in %) were calculated by compartmental analysis. Results: A total of 176 placentas (27 rats) were analyzed by DCE MRI (97 cases and 79 controls). The model was effective, inducing intrauterine growth retardation, as there was a significant difference between the two groups for placental weight (p < 0.01), fetal weight (p = 0.019), and fetal length (p < 0.01). There was no significant difference in placental perfusion between the L-NAME and control groups (140.1 ± 74.1 vs. 148.9 ± 97.4, respectively; p = 0.496). There was a significant difference between the L-NAME and control groups for Vb (53 ± 12.9 vs. 46.7 ± 9%, respectively; p < 0.01). Conclusion: In the L-NAME preeclampsia model, placental perfusion is normal and the fractional blood volume is increased, suggesting that preeclampsia is not always expressed as a result of decreased placental perfusion. This highlights the usefulness of MRI for investigating the physiopathology of preeclampsia.

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Section: Resultssupporting

confidence: 82%

Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Assessment of Placental Perfusion in the Preeclampsia L-NAME Rat Model with High-Field Dynamic Contrast-Enhanced MRI

et al. 2018

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Placental perfusion in uterine ischemia model as evaluated by dynamic contrast enhanced MRI

Drobyshevsky¹,

Prasad

2015

Magnetic Resonance Imaging

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Background To validate DCE MRI method of placental perfusion estimation and to demonstrate application of the method in a rabbit model of fetal antenatal hypoxia-ischemia. Methods Placental perfusion was estimated by dynamic contrast imaging with bolus injection of Gd-DTPA in 3 Tesla GE magnet in a rabbit model of placental ischemia–reperfusion in rabbit dams at embryonic day 25 gestation age. Placental perfusion was measured using steepest slope method on DCE MRI before and after intermittent 40 min uterine ischemia. Antioxidants (n = 2 dams, 9 placentas imaged) or vehicle (n = 5 dams, 23 placenta imaged) were given systemically in a separate group of dams during reperfusion–reoxygenation. Placental perfusion was also measured in two dams from the antioxidant group (10 placentas) and two dams from the control group (12 placentas) by fluorescent microspheres method. Results While placental perfusion estimates between fluorescent microspheres and DCE MRI were significantly correlated (R2 = 0.85; P < 0.01), there was approximately 33% systematic underestimation by the latter technique. DCE MRI showed a significant decrease in maternal placental perfusion in reperfusion–reoxygenation phase in the saline, 0.44 ± 0.06 mL/min/g (P = 0.012, t-test), but not in the antioxidant group, 0.62 ± 0.06 mL/min/g, relative to preocclusion values (0.77 ± 0.07 and 0.84 ± 0.12 mL/min/g, correspondingly). Conclusion Underestimation of true perfusion in placenta by steepest slope DCE MRI is significant and the error appears to be systematic.

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Perfusion of the placenta assessed using arterial spin labeling and ferumoxytol dynamic contrast enhanced magnetic resonance imaging in the rhesus macaque

Ludwig

Fain

Nguyen

et al. 2018

Magnetic Resonance in Med

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Purpose To investigate the correspondence between arterial spin labeling (ASL) flow‐sensitive alternating inversion recovery (FAIR) and ferumoxytol DCE MRI for the assessment of placental intervillous perfusion. Methods Ten pregnant macaques in late second trimester were imaged at 3 T using a 2D ASL FAIR, with and without outer‐volume saturation pulses used to control the bolus width, and a 3D ferumoxytol DCE‐MRI acquisition. The ASL tagged/control pairs were averaged, subtracted, and normalized to create perfusion ratio maps. Contrast arrival time and uptake slope were estimated by fitting the DCE data to a sigmoid function. Macaques (N = 4) received interleukin‐1β to induce inflammation and disrupt perfusion. Results The FAIR tag modification with outer‐volume saturation reduced the median ASL ratio percentage compared with conventional FAIR (0.64% ± 1.42% versus 0.71% ± 2.00%; P < .05). Extended ferumoxytol arrival times (34 ± 25 seconds) were observed across the placenta. No significant DCE signal change was measured in fetal tissue (−0.6% ± 3.0%; P = .52) or amniotic fluid (1.9% ± 8.8%; P = .59). High ASL ratio was significantly correlated with early arrival time and high uptake slope (P < .05), but ASL signal was not above noise in late‐DCE‐enhancing regions. No significant differences were observed in perfusion measurements between the interleukin‐1β and controls (P > .05). Conclusion The ASL‐FAIR and ferumoxytol DCE‐MRI methods are feasible to detect early blood delivery to the macaque placenta. Outer volume saturation reduced the high macrovascular ASL signal. Interleukin‐1β exposure did not alter placental intervillous perfusion. An endogenous‐labeling perfusion technique is limited due to extended transit times for flow within the placenta beyond the immediate vicinity of the maternal spiral arteries.

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Measurement of Placental Perfusion by Dynamic Contrast-Enhanced MRI at 4.7 T

Cited by 23 publications

References 58 publications

Assessment of Placental Perfusion in the Preeclampsia L-NAME Rat Model with High-Field Dynamic Contrast-Enhanced MRI

Assessment of Placental Perfusion in the Preeclampsia L-NAME Rat Model with High-Field Dynamic Contrast-Enhanced MRI

Placental perfusion in uterine ischemia model as evaluated by dynamic contrast enhanced MRI

Perfusion of the placenta assessed using arterial spin labeling and ferumoxytol dynamic contrast enhanced magnetic resonance imaging in the rhesus macaque

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