Measurement of Photosynthetic Oxygen Evolution

Gorkom, Hans J. van; Gast, P.

doi:10.1007/0-306-47960-5_24

Cited by 15 publications

(6 citation statements)

References 65 publications

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“…Photochemical functions: An oxygen electrode attached with a logger was used to measure the activity of photosynthetic oxygen evolution according to Van Gorkom and Gast (1996) and the activity of Hill-reaction as described by Kutík et al (1999). The photophosphorylation activity was measured according to Ketcham et al (1984).…”

Section: Methodsmentioning

confidence: 99%

Effects of drought on photosynthetic characteristics of flag leaves of a newly-developed super-high-yield rice hybrid

Chen¹,

Liu²,

Zhang³

et al. 2004

Photosynt.

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We investigated responses of chloroplasts from flag leaves of a newly-developed super-high-yield rice (Oryza sativa L.) hybrid LiangYouPeiJiu (LYPJ) to water stress (withholding irrigation) during the grain-filling period. In the early stage of water stress (0-6 d) only the activity of Hill reaction was inhibited, whereas activities of photophosphorylation and Ca 2+ -ATPase, and ATP content were increased and peaked in the day 6 of withholding irrigation. In the late stage of water stress (6-12 d), the activities of photosynthetic O 2 evolution, Hill reaction, photophosphorylation, and Ca 2+ -ATPase, and ATP content were significantly reduced. The membrane lipid content was sharply decreased, especially of sulfoquinovosyl-diacylglycerol (SQDG) and phosphatidylglycerol (PG). The changes in the ultrastructure of chloroplasts included mainly a decrease in number of grana and increase in number of osmiophilic granules.

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Section: Methodsmentioning

confidence: 99%

Effects of drought on photosynthetic characteristics of flag leaves of a newly-developed super-high-yield rice hybrid

Chen¹,

Liu²,

Zhang³

et al. 2004

Photosynt.

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“…To better delineate the cause of initial toxicity associated with high GBF concentrations, we measured maximal oxygen evolution rates for strains briefly exposed to media with 12.5% (vol/vol) GBF while increasing the light intensity. Measurements of levels of photosynthetic oxygen evolution would allow an indirect assessment of PSII activity and electron transfer ( 37 ). Cultures were grown to the early linear phase in medium A + or GBF media, washed, and resuspended in the appropriate media.…”

Section: Resultsmentioning

confidence: 99%

Inhibition of Cyanobacterial Growth on a Municipal Wastewater Sidestream Is Impacted by Temperature

Korosh

Dutcher

Pfleger

et al. 2018

mSphere

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Cyanobacteria are viewed as promising platforms to produce fuels and/or high-value chemicals as part of so-called “biorefineries.” Their integration into wastewater treatment systems is particularly interesting because removal of the nitrogen and phosphorus in many wastewater streams is an expensive but necessary part of wastewater treatment. In this study, we evaluated strategies for cultivating Synechococcus sp. strain PCC 7002 on media comprised of two wastewater streams, i.e., treated secondary effluent supplemented with the liquid fraction extracted from sludge following anaerobic digestion. This strain is commonly used for metabolic engineering to produce a variety of valuable chemical products and product precursors (e.g., lactate). However, initial attempts to grow PCC 7002 under otherwise-standard conditions of light and temperature failed. We thus systematically evaluated alternative cultivation conditions and then used multiple methods to dissect the apparent toxicity of the media under standard cultivation conditions.

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“…In the following a brief description of the underlying principles of different methods will be presented (see also van Gorkom and Gast 1996):…”

Section: Detection Methods Of Molecular Dioxygenmentioning

confidence: 99%

“…(Subczynski and Hyde 1981;Swarts and Pals 1989; for a review see van Gorkom and Gast 1996). The method is based on the interaction of paramagnetic 3 R g O 2 with free radicals thus giving rise to changes of the relaxation times (T 1 and T 2 ) of these species, usually via a mechanism due to the Heisenberg exchange effect (see Windrem and Plachy 1980 and references therein).…”

Section: Oximetymentioning

confidence: 99%

Oxygen detection in biological systems

Ренгер

Hanssum²

2009

Photosynth Res

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This article presents a brief description of analytical tools for monitoring evolution and consumption of molecular dioxygen in biological organisms. Based on its nature as a gas and its physical and chemical properties of the ground state ³Σ(g)O₂; different approaches have been developed for quantitative determinations: (i) manometry, (ii) formation of titratable sediments, (iii) solid state electrodes, (iv) EPR oximetry, (v) luminescence quenching, (vi) biological sensoring, (vii) mass spectrometry and (viii) amperometry. Among these methods mass spectrometry and amperometry are of special relevance for studies on the mechanisms of photosynthetic dioxygen evolution. Mass spectrometry is described in the article of Beckman et al. in this special issue. Therefore, the major part of this contribution focuses on amperometric methods that are currently widely used. Two different types of electrodes are described: (i) Clark-type electrode and (ii) Joliot-type electrode. The complementary advantages of both systems are outlined. A more detailed description comprises the potential of the Joliot-type electrode for mechanistic studies on the reactivity of the different redox states of the water oxidizing complex (WOC).

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Measurement of Photosynthetic Oxygen Evolution

Cited by 15 publications

References 65 publications

Effects of drought on photosynthetic characteristics of flag leaves of a newly-developed super-high-yield rice hybrid

Effects of drought on photosynthetic characteristics of flag leaves of a newly-developed super-high-yield rice hybrid

Inhibition of Cyanobacterial Growth on a Municipal Wastewater Sidestream Is Impacted by Temperature

Oxygen detection in biological systems

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