Measurement of Low Energy Beta-Emitters in Aqueous Solution by Liquid Scintillation Counting of Emulsions.

Effects of hormones on the rate of the triacylglycerol/fatty acid substrate cycle in adipocytes and epididymal fat pads

“…Fatty acids were removed from the hydrolysate by 2 extractions using 5 ml 40-60 LETTERS September 1982 fraction petroleum ether. A volume of the aqueous hydrolysate (0.25 ml) was dissolved in 10 ml of scintillant (which contained 2.0 g 2,5-diphenyloxazole (PPO) and 0.05 g 1,4-bis(5-phenyloxazolyl)-2-benzene (POPOP) in 500 ml toluene + 250 ml Triton X-100 [11]) and the radioactivity (i.e., [3H]-glycerol) was measured in a liquid scintillation counter (Beckman model LS 200). The petroleum ether washings were combined in a scintillation vial and evaporated to dryness, after which 10 ml scintillant (which contained 3.0 g PPO/1 and 0.1 g POPOP/1 toluene) was added and radioactivity (i.e., [3H]fatty acid) was measured.…”

The sensitivity of a malignant cell line to hyperthermia (42°C) at low intracellular pH

“…For radioactive counting, one quarter of the final sample in each tube obtained as described above was transferred to a counting vial and its activity determined using the toluene: Triton X-100 scintillation mixture of Patterson and Greene (1965). The samples were neutralized before addition of the counting fluid, and an internal standard of methanol-14C was used to correct for quenching.…”

Interaction of SH‐reagents with the ribosomal 30 S subparticle and ‘non‐enzymatic’ translocation

“…The proteins were separated by two-dimensional polyacrylamide gel electrophoresis according to Kaltschmidt and Wittmann [7].The stained regions of the gel corresponding to all the individual proteins of the 30 S subparticle were cut out, dried and then dissolved in 30% H,O, with NH,OH [8]; the radioactivity of each protein was counted in a Packard scintillation spectrometer in a toluene-PPO-POPOP-Triton X-100 (2: 1) mixture [9]. Fig.…”