Measurement of Local Cerebral Blood Flow with [¹⁴C]Iodoantipyrine in the Mouse

Abstract

Local cerebral blood flow was measured in the mouse by means of the [14C]iodoantipyrine method. This method has been previously used in the monkey, dog, cat, and rat, but its application to small mammals such as the mouse requires special attention to potential sources of error. The small size of the mouse brain requires special attention to the rapid removal and freezing of the brain to minimize effects of postmortem diffusion of tracer in the tissue. Because of the relatively low diameter/length ratios of the catheters needed for arterial sampling in small animals, substantial errors can occur in the determination of the time course of the [14C]iodoantipyrine concentration in the arterial blood unless corrections for lag time and dead space washout in the catheter are properly applied. Local cerebral blood flow was measured in seven awake mice with appropriate care to minimize these sources of error. The values were found to vary from 48 ml/100 g/min in the corpus callosum to 198 ml/100 g/min in the inferior colliculus. The results demonstrate that the [14C]iodoantipyrine method can be used to measure local cerebral blood flow in the mouse and that the values in that species are, in general, somewhat higher than those in the rat.

Reduced Cerebral Blood Flow but Elevated Cerebral Glucose Metabolic Rate in Erythropoietin Overexpressing Transgenic Mice with Excessive Erythrocytosis

“…In each group, 16 animals were randomized to be used either for the autoradiographic determination of local CBF (n = 8) or for the measurement of local CMR glc (n = 8), according to the methods described in the rat (Sakurada et al, 1978;Sokoloff et al, 1977). These autoradiographic methods were adapted to mice according to the previous use in this species (Jay et al, 1985(Jay et al, , 1988.…”

“…The washout correction rate constant was 40/min (Jay et al, 1988). The brain-blood partition coefficient for iodo [ 14 C]antipyrine was measured by incubation of mouse brain slices of 50 mm thickness with whole mouse blood (n =4) of a defined radioactivity as previously described (Waschke et al, 1993).…”

Postnatal Changes in Local Cerebral Blood Flow Measured by the Quantitative Autoradiographic [14C]Iodoantipyrine Technique in Freely Moving Rats

“…The correction for both the time lag and the washout effect was controlled in separate experiments, according to the procedure described by Jay et al (1988). In these experiments, Clay Adams PE-lO or PE-50 catheters 150 mm long were filled with nonradioactive heparinized blood and connected to a syringe filled with blood con taining 0.2 !LCi/ml of [14C]IAP.…”

“…However, their experiments were done on rats with no indication of age or weight. Likewise, Jay et al (1988) published their data on the measurement of LCBF rates in mouse by means of the [14C]iodoantipyrine method. These authors mainly focused their attention on the poten tial sources of errors in the measurement of LCBF in small animals, especially on those related to the dead space washout in the arterial catheter.…”

Massive Inborn Angiogenesis in the Brain Scarcely Raises Cerebral Blood Flow

“…Ten minutes before the decapitation in the CBF and CGU experiments, 0.2 mL of 4% Evans blue in saline was injected. CBF was measured according to the autoradiographic method of Sakurada et al (1978) and the modifications of Jay et al (1988). To this end 125…”