Measurement of layer-specific mechanical properties in multilayered biomaterials by micropipette aspiration

Zhao, Ruogang; Sider, Krista L.; Simmons, Craig A.

doi:10.1016/j.actbio.2010.11.004

Cited by 82 publications

(74 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The elastic modulus of the thick gels is estimated to be $ 25 kPa . This is at the upper end of the range of focal elastic moduli of the fibrosa layer of normal aortic valve leaflets; most regions of the fibrosa and all of the ventricularis are more compliant Zhao et al, 2011). Since cells tend to match their stiffness to that of their matrix (Solon et al, 2007), the higher stiffness of the fibroblastic VICs compared with primary cells may be due to the thick gels being stiffer than most of the native valve ECM.…”

Section: Discussionmentioning

confidence: 97%

“…Thick gels provide a compliant substrate ( $ 25 kPa) while thin gels provide a stiff substrate ( $ 110 kPa), as described previously . The modulus of the compliant substrate is similar to that of the stiffest regions of normal valves Zhao et al, 2011), and the modulus of the stiff substrates is estimated to be roughly that of stenotic leaflets (Imanaka et al, 2007). The gel measurements and their relationship to the mechanical properties of native leaflet tissue are discussed further in the Supplemental Material.…”

Section: Collagen Matricesmentioning

confidence: 99%

“…Mechanical stimuli appear to contribute importantly to disease initiation and/or progression, as there are spatial correlations between the valve mechanical environment and the leaflet regions where lesions form. For example, lesions form preferentially in regions exposed to large bending stresses (Thubrikar et al, 1986) and disturbed blood flow (Simmons et al, 2005), and in the fibrosa layer, which is the stiffest of the three layers of the leaflet (Vesely and Noseworthy, 1992;Zhao et al, 2011). The ability of VICs to sense and respond to these tissue-level stimuli depends on several factors, including their inherent cellular-level mechanical properties.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The elastic properties of valve interstitial cells undergoing pathological differentiation

Wyss

Yip

Mirzaei

et al. 2012

Journal of Biomechanics

Self Cite

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 97%

Section: Collagen Matricesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The elastic properties of valve interstitial cells undergoing pathological differentiation

Wyss

Yip

Mirzaei

et al. 2012

Journal of Biomechanics

Self Cite

View full text Add to dashboard Cite

“…Since cell adhesion and junction are the main mechanisms for in vivo tissue development, an examination of AFs in differentiated cells is important for the determination of tissue structure formation [31]. We hypothesized that E-cad transfection may enhance the adhesion in differentiated cells and, therefore, induce greater AFs in EBs in EC-ESC.…”

Section: E-cad Enhances the Adhesion In Differentiated Cells And Facimentioning

confidence: 99%

Epithelial-Mesenchymal Transition Delayed by E-cad to Promote Tissue Formation in Hepatic Differentiation of Mouse Embryonic Stem Cells In Vitro

Shang

et al. 2014

Stem Cells and Development

View full text Add to dashboard Cite

Hepatic differentiation of embryonic stem cells (ESCs) usually results in a single cell lineage, and the formation of liver tissues remains difficult. Here, we examine the role of epithelial-mesenchymal transition (EMT) that is regulated by epithelial cadherin (E-cad) expression in hepatic tissue formation from ESCs. E-cad was transfected into mouse ESCs to enable a stable expression of E-cad. Hepatic differentiation of ESCs was then induced by hepatic growth factors. Wnt/β-catenin signaling and EMT speed were examined to determine the differentiation process. Hepatic and angiogenesis markers, as well as differentiated cell-adhesive force were also examined to identify the hepatic tissue differentiation. In our results, E-cad expression gradually decreased in normal ESC (N-ESC) differentiation, but remained stable in the E-cad transfected ESC (EC-ESC) group. In EC-ESC differentiation, expressions of cytoplastic β-catenin and EMT were much lower and significantly prolonged. Angiogenesis markers vascular endothelial growth factor receptor-1 (VEGFR-1) and CD31/PECAM-1 were expressed only on day 5-13 in N-ESC differentiation, whereas VEGFR-1 and CD31/PECAM-1 were expressed prolonged on day 5-17 in the EC-ESC group and were coincident with the expression of hepatic markers. Finally, EC-ESC differentiation maintained multilayer-growth patterns, and abundant vascular network structures appeared and migrated in albumin-positive cell areas. The cellular adhesion forces between embryonic body cells in EC-ESC differentiation during day 13-17 were similar to those of mouse liver tissue. In conclusion, accelerated EMT due to the decreased E-cad expression may partially contribute to the failure of hepatic tissue formation in N-ESC differentiation. E-cad can act in synergy with hepatic growth factors and facilitate the early-stage formation of hepatic tissues through down-regulating Wnt/β-catenin signaling and delaying EMT. This work provides a new insight into hepatic tissue differentiation that is mediated by E-cad from ESC.

show abstract

“…In this sense, measuring the local mechanical properties of biopolymers is of great impor tance. The micropipette aspiration [5][6][7][8][9][10][11][12][13][14] or nanoindentation methods [15][16][17][18][19][20][21][22][23] can be used for this purpose and the present study is concerned with the latter.…”

Section: Introductionmentioning

confidence: 99%

On the Applicability of Sneddon's Solution for Interpreting the Indentation of Nonlinear Elastic Biopolymers

Zhang

Chen

Feng

et al. 2014

Journal of Applied Mechanics

View full text Add to dashboard Cite

Indentation has been widely used to characterize the mechanical properties of biopolymers. Besides Hertzian solution, Sneddon's solution is frequently adopted to interpret the indentation data to deduce the elastic properties of biopolymers, e.g., elastic modulus. Sneddon's solution also forms the basis to develop viscoelastic contact models for determining the viscoelastic properties of materials from either conical or flat punch indentation responses. It is worth mentioning that the Sneddon's solution was originally proposed on the basis of linear elastic contact theory. However, in both conical and flat punch indentation of compliant materials, the indented solid may undergo finite deformation. In this case, the extent to which the Sneddon's solution is applicable so far has not been systematically investigated. In this paper, we use the combined theoretical, computational, and experimental efforts to investigate the indentation of hyperelastic compliant materials with a flat punch or a conical tip. The applicability of Sneddon's solutions is examined. Furthermore, we present new models to determine the elastic properties of nonlinear elastic biopolymers.

show abstract

Measurement of layer-specific mechanical properties in multilayered biomaterials by micropipette aspiration

Cited by 82 publications

References 26 publications

The elastic properties of valve interstitial cells undergoing pathological differentiation

The elastic properties of valve interstitial cells undergoing pathological differentiation

Epithelial-Mesenchymal Transition Delayed by E-cad to Promote Tissue Formation in Hepatic Differentiation of Mouse Embryonic Stem Cells In Vitro

On the Applicability of Sneddon's Solution for Interpreting the Indentation of Nonlinear Elastic Biopolymers

Contact Info

Product

Resources

About