Measurement of K vitamins in human and animal feces by high-performance liquid chromatography with fluorometric detection.

Sakano, Toshiyuki; Nagaoka, Tadayoshi; Morimoto, Akira; Hirauchi, Kazumasa

doi:10.1248/cpb.34.4322

Cited by 50 publications

(22 citation statements)

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“…Up to 12 different types have been described, from MK-4 to MK-15 [5]. The most common MKs in humans are the short-chain MK-4, which is the only MK produced by systemic conversion of phylloquinone to menaquinones, and the long-chain vitamers, MK-7 through MK-10, which are synthesized by bacteria in humans.…”

Section: Introductionmentioning

confidence: 99%

Vitamin K plasma levels determination in human health

Fusaro

Gallieni

Rizzo

et al. 2017

Clinical Chemistry and Laboratory Medicine (CCLM)

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Vitamin K (phylloquinone or vitamin K 1 and menaquinones or vitamin K 2 ) plays an important role as a cofactor in the synthesis of hepatic blood coagulation proteins, but recently has also aroused an increasing interest for its action in extra-hepatic tissues, in particular in the regulation of bone and vascular metabolism. The accurate measurement of vitamin K status in humans is still a critical issue. Along with indirect assays, such as the undercarboxylated fractions of vitamin K-dependent proteins [prothrombin, osteocalcin (OC), and matrix gla protein], the direct analysis of blood levels of phylloquinone and menaquinones forms might be considered a more informative and direct method for assessing vitamin K status. Different methods for direct quantification of vitamin K serum levels are available. High-performance liquid chromatography (HPLC) methods coupled with post-column reduction procedures and fluorimetric or electrochemical detection are commonly used for food and blood analysis of phylloquinone, but they show some limitations when applied to the analysis of serum menaquinones because of interferences from triglycerides. Recent advancements include liquid chromatography tandem mass spectrometry (LCMS/MS) detection, which assures higher specificity. The optimization and standardization of these methods requires specialized laboratories. The variability of results observed in the available studies suggests the need for further investigations to obtain more accurate analytical results.

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Section: Introductionmentioning

confidence: 99%

Vitamin K plasma levels determination in human health

Fusaro

Gallieni

Rizzo

et al. 2017

Clinical Chemistry and Laboratory Medicine (CCLM)

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“…MK-7 is produced by fermentation with Bacillus species and can be obtained from food products such as cheese, meat and fermented soybean, but the concentration is very low. Natto is a traditional fermented soybean food in Japan; it contains comparatively high amounts of MK-7 (about 800-900 µg/100 g natto) compared with other foods (Sakano et al 1988). Since the strains of B. subtilis used for manufacturing natto are edible, they are among the best sources of MK in the food industry.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Production of Vitamin K2 from Bacillus subtilis (natto) by Mutation and Optimization of the Fermentation Medium

Song¹,

Liu²,

Wang³

et al. 2014

Braz. arch. biol. technol.

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“…On the other hand , from the nutritional perspective, the prospective cohort study including both premenopausal and postmeno pausal women shows that dietary intake of vitamin K from vegetables, namely vitamin Kl intake , reduces the risk of femoral neck fracture (8). Soybeans fermented by Bacillus Natto (Natto), a traditional Japanese food , con taro more than 100 times more vitamin K2 (mainly MK-7) than various cheeses (9). It has been reported that intake of Natto increases serum level of vitamin K2, especially MK-7, and Gla in normal individuals (10).…”

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confidence: 99%

Promotion of Bone Formation by Fermented Soybean (Natto) Intake in Premenopausal Women

Katsuyama¹,

Ideguchi²,

Fukunaga

et al. 2004

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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SummaryA therapeutic agent of vitamin K2 is approved for the treatment of osteoporosis in Japan. However, little is known about the efficacy of dietary intake of vitamin K2 for bone health. We compared the effects of various levels of fermented soybeans (Natto) intake , which contains plenty of vitamin K2, on bone stiffness and bone turnover markers in healthy premenopausal women, Seventy-three healthy premenopausal women were ran domly divided into four groups matched for age and parity categories . Natto was supplied as follows: Group 1(no intake), Group 2 (once per month), Group 3 (once per week) and Group 4 (three times per week). Subjects took Natto at a lunch for 1 y , and the stiffness index by quantitative ultrasound and bone turnover markers were assessed at baseline, 6 mo and 1 y There was no statistical difference in the stiffness index during the 1 y observation . How ever, bone specific alkaline phosphatase (BAP) in Group 4 was higher than that in Group 3 at 1 y and undercarboxylated osteocalcin (Glu) in Group 4 was significantly lower than those in Groups 1, 2 and 3 at 6 mo. Logistic regression analysis showed that the risk of reduction of bone formation markers declined to 0.07 in Group 4 based on that in Group 1 . In premenopausal women who had to keep the stiffness index as high as possible before menopause, Natto intake may have contributed to the promotion of bone formation .

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Measurement of K vitamins in human and animal feces by high-performance liquid chromatography with fluorometric detection.

Cited by 50 publications

References 0 publications

Vitamin K plasma levels determination in human health

Vitamin K plasma levels determination in human health

Enhanced Production of Vitamin K2 from Bacillus subtilis (natto) by Mutation and Optimization of the Fermentation Medium

Promotion of Bone Formation by Fermented Soybean (Natto) Intake in Premenopausal Women

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