Measurement of intact quantal packet of transmitters released from single nerve terminal by loose-patch amperometry

Chen, Pei-Hua; Shen, Xiangrong; Zhao, Shuainan; Liu, Zili; Zhu, Qianwen; Zhu, Tao; Zhang, Shuli; Li, Yang; Mao, Lanqun; Sun, Jianyuan

doi:10.1016/j.bios.2021.113143

“…During measurement, the pipette orifice was patched to (sealing resistance >10 MΩ) the presynaptic terminal membrane, and then the inner nanoelectrode approached the releasing sites under the control of a nanomotor. This recording system can effectively collect information on intact synaptic quantal release [16] …”

Section: Single‐cell Exocytosis Monitoringmentioning

confidence: 99%

“…This recording system can effectively collect information on intact synaptic quantal release. [16] To fish for neurotransmitters inside the synaptic cleft, Amatore, Huang and their colleagues worked on the development of nanoelectrode sensors. The cone-shaped nano tip could be sized down to 50-100 nm by manual etching with a heated platinum microwire, and carefully squeezed into the cleft.…”

Section: Synaptic Neurotransmitter Release Monitoringmentioning

confidence: 99%

Multi‐Spatiotemporal Probing of Neurochemical Events by Advanced Electrochemical Sensing Methods

Wu

¹

,

Yu

²

,

Mao³

2022

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Neurochemical events involving biosignals of different time and space dimensionalities constitute the complex basis of neurological functions and diseases. In view of this fact, electrochemical measurements enabling real‐time quantification of neurochemicals at multiple levels of spatiotemporal resolution can provide informative clues to decode the molecular networks bridging vesicles and brains. This Minireview focuses on how scientific questions regarding the properties of single vesicles, neurotransmitter release kinetics, interstitial neurochemical dynamics, and multisignal interconnections in vivo have driven the design of electrochemical nano/microsensors, sensing interface engineering, and signal/data processing. An outlook for the future frontline in this realm will also be provided.

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“…Data for offline analysis were low-pass-filtered at 1 kHz. A level of 53 the RMS of noise at baseline was set as the threshold for signal detection (Chen et al, 2021). Spike amplitude I max is the spike maximum from the baseline current.…”

Section: Amperometric Recording and Analysismentioning

confidence: 99%

Intestinal microbiota modulates adrenomedullary response through Nod1 sensing in chromaffin cells

Chen

¹

,

Chen

²

,

Zhang

³

et al. 2021

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Multi‐Spatiotemporal Probing of Neurochemical Events by Advanced Electrochemical Sensing Methods

Wu

¹

,

Yu

²

,

Mao

³

2022

Self Cite

View full text Add to dashboard Cite

Neurochemical events involving biosignals of different time and space dimensionalities constitute the complex basis of neurological functions and diseases. In view of this fact, electrochemical measurements enabling real‐time quantification of neurochemicals at multiple levels of spatiotemporal resolution can provide informative clues to decode the molecular networks bridging vesicles and brains. This Minireview focuses on how scientific questions regarding the properties of single vesicles, neurotransmitter release kinetics, interstitial neurochemical dynamics, and multisignal interconnections in vivo have driven the design of electrochemical nano/microsensors, sensing interface engineering, and signal/data processing. An outlook for the future frontline in this realm will also be provided.

show abstract

Measurement of intact quantal packet of transmitters released from single nerve terminal by loose-patch amperometry

Cited by 4 publications

References 34 publications

Multi‐Spatiotemporal Probing of Neurochemical Events by Advanced Electrochemical Sensing Methods

Multi‐Spatiotemporal Probing of Neurochemical Events by Advanced Electrochemical Sensing Methods

Intestinal microbiota modulates adrenomedullary response through Nod1 sensing in chromaffin cells

Multi‐Spatiotemporal Probing of Neurochemical Events by Advanced Electrochemical Sensing Methods

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