Measurement of immunoreactive interleukin-1β from human mononuclear cells: Optimization of recovery, intrasubject consistency, and comparison with interleukin-1α and tumor necrosis factor

Endres, Stefan; Ghorbani, R; Lonnemann, Gerhard; Meer, J.W.M. van der; Dinarello, C A

doi:10.1016/0090-1229(88)90130-4

Cited by 169 publications

(78 citation statements)

References 35 publications

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“…For example, LPS-induced TNF-␣ production measured in the two samples showed a correlation (r) of 0.98 (Table I). These data are consistent with previous studies showing reproducible interindividual differences in LPS-induced IL-1␤ (16,18,19), TNF-␣ (15,16,20), and IL-10 production (15). These findings establish the temporal stability of the LPS-induced cytokine expression phenotype in whole blood.…”

Section: High (Lps High ) and Low (Lps Low ) Response Phenotypes Are supporting

confidence: 82%

Identification of High and Low Responders to Lipopolysaccharide in Normal Subjects: An Unbiased Approach to Identify Modulators of Innate Immunity

Wurfel

Park

Radella

et al. 2005

The Journal of Immunology

109

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LPS stimulates a vigorous inflammatory response from circulating leukocytes that varies greatly from individual to individual. The goal of this study was to use an unbiased approach to identify differences in gene expression that may account for the high degree of interindividual variability in inflammatory responses to LPS in the normal human population. We measured LPS-induced cytokine production ex vivo in whole blood from 102 healthy human subjects and identified individuals who consistently showed either very high or very low responses to LPS (denoted lpshigh and lpslow, respectively). Comparison of gene expression profiles between the lpshigh and lpslow individuals revealed 80 genes that were differentially expressed in the presence of LPS and 21 genes that were differentially expressed in the absence of LPS (p < 0.005, ANOVA). Expression of a subset of these genes was confirmed using real-time RT-PCR. Functional relevance for one gene confirmed to be expressed at a higher level in lpshigh, adipophilin, was inferred when reduction in adipophilin mRNA by small interfering RNA in the human monocyte-like cell line THP-1 resulted in a modest but significant reduction in LPS-induced MCP-1 mRNA expression. These data illustrate a novel approach to the identification of factors that determine interindividual variability in innate immune inflammatory responses and identify adipophilin as a novel potential regulator of LPS-induced MCP-1 production in human monocytes.

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Section: High (Lps High ) and Low (Lps Low ) Response Phenotypes Are supporting

confidence: 82%

Identification of High and Low Responders to Lipopolysaccharide in Normal Subjects: An Unbiased Approach to Identify Modulators of Innate Immunity

Wurfel

Park

Radella

et al. 2005

The Journal of Immunology

109

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“…05% sodium azide. TNF- [27] and Il-1 [28] were determined by specific RIA, as described [ 125 I] Bolton-Hunter-labelled cytokines from NEN Dupont (Bad Homburg, Germany) were used as tracers. Cytokines for calibration of the standard curves were provided by the National Institute for Biological Standards and Control, Potters Bar, UK.…”

Section: Measurements Of Tnf-and Il-1mentioning

confidence: 99%

Elevated cell‐associated levels of interleukin 1β and interleukin 6 in inflamed mucosa of inflammatory bowel disease

Gröttrup-Wolfers

Møeller

Karbach

et al. 1996

Eur J Clin Investigation

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The aim of this study was to investigate the involvement of the monocyte‐derived cytokines interleukin 1β (IL‐1β), interleukin 6 (IL‐6) and tumour necrosis factor‐α (TNF‐α) in idiopathic inflammatory bowel disease. Endoscopic biopsies of normal and inflamed intestinal mucosa were obtained from patients with ulcerative colitis (n = 11) and with Crohn’s disease (n = 10). Intestinal mucosal cells were isolated by collagenase digestion. Cell viability, morphology and CD14 expression were determined. To measure cell‐associated cytokine levels, cells were lysed and analysed for IL‐1β and TNF‐α in specific radioimmunoassays and for IL‐6 using a biological assay. Compared with mucosal cells from control patients without inflammatory bowel disease the inflamed intestine in ulcerative colitis and Crohn’s disease displayed markedly enhanced levels of IL‐1β (median 245 pg 10−6 cells, range 30–1275) and IL‐6 (median 22 U 10−6 cells, range 1–298). Non‐inflamed mucosa in patients with ulcerative colitis and Crohn’s disease did not shown elevated levels of IL‐1β (median 50 pg 10−6 cells, range 33–90) or IL‐6 (mean below detection limit of assay, i.e. 1 U 10−6 cells). In contrast, no clear cut difference between inflamed and non‐inflamed mucosa could be detected for TNF‐α. High tissue levels of IL‐6 were associated with a high endoscopic grade of local inflammation. These results suggest that the monocyte‐derived cytokines IL‐1β and IL‐6 are mediators of inflammation in inflammatory bowel disease.

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“…All subjects gave informed consent prior to the study. Separation of PBMC was performed as described elsewhere (22). PBMC were washed twice in saline and suspended in culture medium (RPMI 1640 Dutch modification, ICN Biomedicals, Costa Mesa) supplemented with human serum 5%, gentamicin 1%, l -glutamine 1%, and pyruvate 1%.…”

Section: Experimental Designmentioning

confidence: 99%

Native LDL potentiate TNFα and IL-8 production by human mononuclear cells

Netea

Kullberg

Demacker

et al. 2002

Journal of Lipid Research

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Measurement of immunoreactive interleukin-1β from human mononuclear cells: Optimization of recovery, intrasubject consistency, and comparison with interleukin-1α and tumor necrosis factor

Cited by 169 publications

References 35 publications

Identification of High and Low Responders to Lipopolysaccharide in Normal Subjects: An Unbiased Approach to Identify Modulators of Innate Immunity

Identification of High and Low Responders to Lipopolysaccharide in Normal Subjects: An Unbiased Approach to Identify Modulators of Innate Immunity

Elevated cell‐associated levels of interleukin 1β and interleukin 6 in inflamed mucosa of inflammatory bowel disease

Native LDL potentiate TNFα and IL-8 production by human mononuclear cells

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