Measurement of hydroxyl radical in rat blood vessel by microbore liquid chromatography and electrochemical detection: an on-line microdialysis study

Tsai, Tung Hu; Cheng, Fu Chou; Hung, Ling Chien; Chen, C.F.

doi:10.1016/s0378-4347(99)00367-9

Cited by 24 publications

(6 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There have been several applications of online MD -LC systems reported over the past decade by the Tsai group and others for the in vivo monitoring of drugs [112 -116] , neurotransmitters, neuropeptides, and other important biomolecules, including reactive oxygen species [117,118] . Applications included primarily pharmacokinetic, metabolism, and neurochemical studies.…”

Section: Off -Line Analysis Using Liquid Chromatographymentioning

confidence: 99%

Analytical Considerations for Microdialysis Sampling

Nandi

Kuhnline

Lunte

2011

Applications of Microdialysis in Pharmaceutical Science

View full text Add to dashboard Cite

Section: Off -Line Analysis Using Liquid Chromatographymentioning

confidence: 99%

Analytical Considerations for Microdialysis Sampling

Nandi

Kuhnline

Lunte

2011

Applications of Microdialysis in Pharmaceutical Science

View full text Add to dashboard Cite

“…For determination of nitric oxide metabolites (NO x ¡ ) or dihydroxybenzoic acid (DHBA), a microdialysis probe (CMA/20 20/40 PC; CMA/Microdialysis AB, Stockholm, Sweden) was put into jugular vein/right atrium as described previously (Tsai et al 1999). The NO x ¡ concentrations in the dialysates were measured with the Eicom ENO-20 NO x ¡ analysis system (Eicom, Kyoto) (Togashi et al 1998).…”

Section: Biochemical Determinationmentioning

confidence: 99%

Interleukin-1 receptor antagonist restores homeostatic function and limits multiorgan damage in heatstroke

et al. 2008

View full text Add to dashboard Cite

We attempt to investigate whether interleukin-1 receptor antagonist (IL-1ra) therapy improves survival during heatstroke by attenuating multiorgan dysfunction. Anesthetized rabbits, immediately after the onset of heatstroke, are divided into three major groups and given: nothing, normal saline (1 ml/kg, i.v.), or IL-1ra (200-400 microg per 1 ml/kg, i.v.). They are exposed to ambient temperature of 40 degrees C to induce heatstroke. Another group of rabbits is exposed to room temperature (24 degrees C) and used as normothermic controls. Hyperthermia, hypotension, cerebral ischemia and edema, hepatic and renal failure, increased levels of both nitric oxide metabolites (NO ( x ) (-) ) and dihydroxybenzoic acid (DHBA) in plasma, hyperkalemia, respiratory alkalosis, and metabolic acidosis and hypoxia are all observed in vehicle-treated heatstroke animals. When the vehicle-treated animals undergo heat stress, their survival time values are found to be 12-18 min. Resuscitation with IL-1ra dose-dependently improves survival time (duration, 132-303 min). As compared with vehicle-treated heatstroke rabbits, IL-1ra therapy significantly causes attenuation of heatstroke-induced hyperthermia, hypotension, cerebral ischemia and edema, intracranial hypertension, hepatic and renal dysfunction, NO ( x ) (-) and DHBA overproduction, hyperkalemia, hypoxia, respiratory alkalosis, and metabolic acidosis. The results indicate that IL-1ra therapy may restore tissue blood flow and homeostatic function, and limit multiorgan dysfunction and death in heatstroke.

show abstract

“…doi:10.1016/j.jelechem.2005.01.002 Å OH without a derivation or trapping step [16]. Gas chromatography-mass spectrometry (GC-MS) [17] and high performance liquid chromatography with electrochemical detection (HPLC-ED) [18][19][20] are the most commonly used methods. In HPLC-ED methods, SA has been usually used as the trap to determine Å OH by measuring its hydroxylation products (2,3-dihydroxybenzoic acid, 2,3-DHBA, and 2,5-dihydroxybenzoic acid, 2,5-DHBA).…”

Section: Introductionmentioning

confidence: 99%

Study on production of free hydroxyl radical and its reaction with salicylic acid at lead dioxide electrode

Wang

et al. 2005

Journal of Electroanalytical Chemistry

View full text Add to dashboard Cite

Measurement of hydroxyl radical in rat blood vessel by microbore liquid chromatography and electrochemical detection: an on-line microdialysis study

Cited by 24 publications

References 25 publications

Analytical Considerations for Microdialysis Sampling

Analytical Considerations for Microdialysis Sampling

Interleukin-1 receptor antagonist restores homeostatic function and limits multiorgan damage in heatstroke

Study on production of free hydroxyl radical and its reaction with salicylic acid at lead dioxide electrode

Contact Info

Product

Resources

About