volume 16, issue 1, P29-35 1994
DOI: 10.1016/0141-0229(94)90106-6
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K.G. Tucker, S. Chalder, M. Al-Rubeai, C.R. Thomas

Abstract: A novel automated image analysis method is described for characterizing the viability and morphology of animal cells from suspension cultures. With the aid of the exclusion dye Trypan Blue, the total and viable cell counts and the percentage of dead cells present are found. The area, perimeter, equivalent diameter, and circularity of the projected image of each cell are also measured, allowing the estimation of cell volume. The image analysis method is inherently sensitive, precise, consistent (non-operator de…

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