Measurement of gp210 autoantibodies in sera of patients with primary biliary cirrhosis

Bauer, Alicja; Habior, Andrzej

doi:10.1002/jcla.20170

Cited by 14 publications

(7 citation statements)

References 21 publications

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“…A total of 25 studies on GP210 (19 studies in English [4] , [5] , [9] , [10] , [12] , [13] , [18] – [21] , [26] , [28] – [35] and six in Chinese [6] , [14] , [15] , [17] , [22] , [23] ) were published between 1988 and 2013 ( Table 1 ), and involved a total of 2,806 PBC patients and 7,494 controls. Of these 25 studies, 15 were carried out in Asia [5] , [6] , [9] , [12] – [15] , [17] , [19] , [20] , [22] , [23] , [29] , [31] , [33] , five in Europe [10] , [28] , [30] , [32] , [35] , and five in South America [4] , [18] , [21] , [26] , [34] ; 56% (14 of 25) using immunoblotting and 48% (12 of 25) with the ELISA method (one article [26] was conducted using two methods). A total of 21 studies on SP100 (13 in English [4] , [8] , [9] , [10] , [12] , [18] – …”

Section: Resultsmentioning

confidence: 99%

Meta-Analysis Assessment of GP210 and SP100 for the Diagnosis of Primary Biliary Cirrhosis

Zhao²,

et al. 2014

PLoS ONE

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PurposeTo conduct a systematic review of included studies assessing the association of GP210 and SP100 with the risk of primary biliary cirrhosis (PBC) using meta-analysis.MethodsFive databases, the Cochrane Library, MEDLINE, VIP, CNKI, WANFANG were used to detect the role of GP210 and SP100 in diagnosis of PBC. Approximately 13,000 participants from several countries were included in this analysis. Meta-DiSc statistical software was used for analysis.Results25 studies on GP210 and 21 studies on SP100 were included in the meta-analysis. The DOR, sensitivity, specificity of GP210 in diagnosis of PBC were 24.854 (11.957–51.660), 0.272 (0.257–0.288), 0.985 (0.982–0.988), respectively, and they were 9.133 (4.739–17.600), 0.231 (0.213–0.249), 0.977 (0.973–0.981) for SP100.ConclusionOur meta-analysis indicated both GP210 and SP100 had high specificity but low sensitivity in diagnosis of PBC.

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Section: Resultsmentioning

confidence: 99%

Meta-Analysis Assessment of GP210 and SP100 for the Diagnosis of Primary Biliary Cirrhosis

Zhao²,

et al. 2014

PLoS ONE

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“…The ANA pattern in PBC includes multiple nuclear dots (MND-ANA) and nuclear membrane-associated ANAs (NM-ANAs). About 30% of patients manifest anti-nuclear envelope (NE) positivity [ 13 , 14 , 15 , 16 ]. In the majority of cases, anti-NM antibodies target gp210 and p62 proteins.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Autoantibodies against Promyelocytic Leukemia Nuclear Body Components and Biochemical Parameters in Sera of Patients with Primary Biliary Cholangitis

et al. 2021

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Primary biliary cholangitis (PBC) is a chronic autoimmune liver disease characterized by immune-mediated destruction of intrahepatic bile ducts and the presence of specific antibodies. The aim of the study was to examine the diagnostic significance of antibodies against promyelocytic leukemia nuclear body (PML NB) components such as Sp100, Sp140, and PML in a cohort of PBC patients and compare the results with biochemical and histological parameters. Serum samples were collected from 93 PBC patients. Anti-Sp100 and anti-PML antibodies were assessed using commercially available kits, anti-Sp140 using developed “in-house” ELISA test. Anti-Sp140, anti-Sp100, and anti-PML antibodies were present in 25 (27%), 37 (40%), and 29 (31%) PBC patients, respectively. Anti-PML NB positive patients also showed increased concentration of bilirubin and alkaline phosphatase (p < 0.05). In the group with the presence of at least two types of these antibodies, more frequent deaths or transplantations were observed. A correlation between the presence of antibodies and histological grade (OR = 2.55 p = 0.039) was established. Patients with bilirubin > 1.1 mg/dL at the time of diagnosis had a significantly shorter time of survival than patients with bilirubin ≤ 1.1 mg/dL (HR 5.7; 95% C.I., 2.7, 12.3; p < 0.001). Our data confirm very high specificity of anti-PML NB antibodies, which can expand the laboratory diagnostic capabilities of PBC. We found an association between positive reactivity of autoantibodies directed against components of PML nuclear bodies and higher concentrations of bilirubin and alkaline phosphatase, but the main prognostic marker of survival remains serum bilirubin.

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“…The nuclear rim pattern is indicative of antibodies directed against components of the nuclear envelope and nuclear pore complex, with gp210 being the primary target antigen in PBC [38]. Gp210 antibodies were found in approximately 25% of patients with PBC, had a specificity of greater than 95% [36,39] and were associated with an increased risk of hepatic failure [10,40,41] and a higher Mayo risk score [42]. In the present study, 27% of our PBC cohort reacted with gp210, but there was no significant association with a higher Mayo risk score or liver decompensation.…”

Section: Discussionmentioning

confidence: 99%

Autoantibodies to GW bodies and other autoantigens in primary biliary cirrhosis

Stinton

Swain

Myers

et al. 2010

Clinical and Experimental Immunology

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SummaryAutoantibodies to intracellular targets in mitochondria and nuclei are serological hallmarks of primary biliary cirrhosis (PBC). One of the most recently identified cellular targets of PBC autoantibodies is a novel cytoplasmic structure referred to as GW bodies [GWB, G (glycine) W (tryptophan)-containing bodies (GWB)]. GWB are indentified as discrete cytoplasmic domains that are involved in mRNA processing via the RNA interference (RNAi) pathway. Key components of GWB include the proteins GW182, Ago2, RNA-associated protein 55 (RAP55) and Ge-1/Hedls. The primary objective was to study the frequency and clinical association of antibodies directed to GWB components, in 109 PBC patients. Autoantibodies to mitochondrial antigen-pyruvate dehydrogenase complex (M2), branched-chain 2-oxo-acid dehydrogenase complex and 2-oxo glutarate dehydrogenase complex (3E-BPO), gp210, sp100, promyelocytic leukaemia cell antigen (PML) and liver kidney microsomal-1 antigen (LKM-1) were detected by a line immunoassay and antibodies to GWB (GW182, RAP55, Ge-1, GW2, GW3) and glutamate receptor interacting protein (GRIP)-associated protein-1 (GRASP-1), by an addressable laser bead immunoassay (ALBIA). The most common GWB autoantigen targets were: RAP55-28%, GW182-12%, GW2-2% and antibodies to GRASP-1-17%. By comparison, the frequency of reactivity to established PBC autoantigens was: gp210, 27%; sp100, 27% and PML, 17%. None of the autoantibodies were associated with differences in Mayo risk score or liver decompensation. This study is the first study to show that antibodies to RAP55, GW182 and GRASP-1 are the most common GWB targets in PBC.

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Measurement of gp210 autoantibodies in sera of patients with primary biliary cirrhosis

Cited by 14 publications

References 21 publications

Meta-Analysis Assessment of GP210 and SP100 for the Diagnosis of Primary Biliary Cirrhosis

Meta-Analysis Assessment of GP210 and SP100 for the Diagnosis of Primary Biliary Cirrhosis

Analysis of Autoantibodies against Promyelocytic Leukemia Nuclear Body Components and Biochemical Parameters in Sera of Patients with Primary Biliary Cholangitis

Autoantibodies to GW bodies and other autoantigens in primary biliary cirrhosis

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