Measurement of glycosylated ferritin with Concanavalin A: Assay design, optimization and validation

Raynor, Alexandre; Peoc’h, Katell; Boutten, Anne

doi:10.1016/j.jchromb.2022.123184

Cited by 3 publications

(1 citation statement)

References 16 publications

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“…The GF fraction was determined using Worwood’s procedure [ 20 ], with minor modifications previously described and validated [ 21 ]. This method separated ferritin glyco-forms based on their affinity for concanavalin-A (Con-A).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Glycosylated Ferritin in Adult-Onset Still’s Disease and Differential Diagnoses

Guerber

Garneret

Jammal

et al. 2022

JCM

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Glycosylated ferritin (GF) has been reported as a good diagnostic biomarker for adult-onset Still’s disease (AOSD), but only a few studies have validated its performance. We performed a retrospective study of all adult patients with at least one GF measurement over a 2-year period in one hospital laboratory. The diagnosis of AOSD was based on the expert opinion of the treating physician and validated by two independent investigators. Patients’ characteristics, disease activity, and outcome were recorded and compared. Twenty-eight AOSD and 203 controls were identified. Compared to controls, the mean GF was significantly lower (22.3% vs. 39.3, p < 0.001) in AOSD patients. GF had a high diagnostic accuracy for AOSD, independent of disease activity or total serum ferritin (AUC: 0.674 to 0.915). The GF optimal cut-off value for AOSD diagnosis was 16%, yielding a specificity of 89% and a sensitivity of 63%. We propose a modified diagnostic score for AOSD, based on Fautrel’s criteria but with a GF threshold of 16% that provides greater specificity and increases the positive predictive value by nearly 5 points. GF is useful for ruling out differential diagnoses and as an appropriate classification criterion for use in AOSD clinical trials.

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Section: Methodsmentioning

confidence: 99%