Measurement of ferritin and anti‐ferritin autoantibodies in serum and colostrum of <scp>H</scp>olstein and <scp>J</scp>apanese <scp>B</scp>lack cows

Kanno, Yoshiya; Ohtsuka, Hiromichi; Yoshikawa, Yasunaga; Watanabe, Kiyotaka; Orino, Koichi

doi:10.1111/asj.12034

Cited by 3 publications

(9 citation statements)

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“…Ferritin is present in blood and extracellular fluids, including milk. In cows it was found that ferritin concentrations were higher in colostrum than in serum [75], in agreement with [57] who reported a higher ferritin genes expression in human colostrum with respect to mature milk. The expression of M-SAA3.2 gene in the bovine mammary gland was reported by [76] who confirmed the role of this gene in host defense, likely in the neonate gastrointestinal tract.…”

Section: Discussionsupporting

confidence: 80%

RNA-Sequencing for profiling goat milk transcriptome in colostrum and mature milk

Crisà

Ferré

Chillemi³

et al. 2016

BMC Vet Res

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BackgroundIn this work we aimed at sequencing and assembling the goat milk transcriptome corresponding at colostrum and 120 days of lactation. To reconstruct transcripts we used both the genome as reference, and a de novo assembly approach. Additionally, we aimed at identifying the differentially expressed genes (DEGs) between the two lactation stages and at analyzing the expression of genes involved in oligosaccharides metabolism.ResultsA total of 44,635 different transcripts, organized in 33,757 tentative genes, were obtained using the goat genome as reference. A significant sequence similarity match was found for 40,353 transcripts (90%) against the NCBI NT and for 35,701 (80%) against the NR databases. 68% and 69% of the de novo assembled transcripts, in colostrum and 120 days of lactation samples respectively, have a significant match with the merged transcriptome obtained using Cufflinks/Cuffmerge. CSN2, PAEP, CSN1S2, CSN3, LALBA, TPT1, FTH1, M-SAA3, SPP1, GLYCAM1, EEF1A1, CTSD, FASN, RPS29, CSN1S1, KRT19 and CHEK1 were found between the top fifteen highly expressed genes. 418 loci were differentially expressed between lactation stages, among which 207 and 122 were significantly up- and down-regulated in colostrum, respectively. Functional annotation and pathway enrichment analysis showed that in goat colostrum somatic cells predominate biological processes involved in glycolysis, carbohydrate metabolism, defense response, cytokine activity, regulation of cell proliferation and cell death, vasculature development, while in mature milk, biological process associated with positive regulation of lymphocyte activation and anatomical structure morphogenesis are enriched. The analysis of 144 different oligosaccharide metabolism-related genes showed that most of these (64%) were more expressed in colostrum than in mature milk, with eight expressed at very high levels (SLCA3, GMSD, NME2, SLC2A1, B4GALT1, B3GNT2, NANS, HEXB).ConclusionsTo our knowledge, this is the first study comparing goat transcriptome of two lactation stages: colostrum and 120 days. Our findings suggest putative differences of expression between stages and can be envisioned as a base for further research in the topic. Moreover because a higher expression of genes involved in immune defense response, carbohydrate metabolism and related to oligosaccharide metabolism was identified in colostrum we here corroborate the potential of goat milk as a natural source of lactose-derived oligosaccharides and for the development of functional foods.Electronic supplementary materialThe online version of this article (doi:10.1186/s12917-016-0881-7) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 80%

RNA-Sequencing for profiling goat milk transcriptome in colostrum and mature milk

Crisà

Ferré

Chillemi³

et al. 2016

BMC Vet Res

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“…Although Kanno et al . () developed direct semi‐quantitative measurement of autoantibodies (IgG, IgM and IgA) to ferritin in the sera and colostrum of bovines with ferritin‐coated plates, in this study, the measurement of immunoglobulin classes with ferritin‐binding activity with co‐immunoprecipitation method has been developed. Ferritin is hypothesized to naturally occur as a heteropolymer, suggesting that H‐ or L‐subunit homopolymers are not naturally found.…”

Section: Discussionmentioning

confidence: 97%

“…In bovines, colostral ferritin and maternal autoantibodies (IgG and IgM) to ferritin are likely to be transferred to newborn calves (Kanno et al . ). Colostrum‐fed foals showed a sharp increase in ferritin antibody activities after 2 weeks of age, suggesting that maternal anti‐ferritin autoantibody is passively transferred via colostrum (Wilson et al .…”

Section: Discussionmentioning

confidence: 99%

“…; Kanno et al . ). However, although the biochemical properties of equine colostral ferritin remain to be elucidated, high serum iron and ferritin levels in foals just after birth are likely to be as a result of the intake of colostrum (Harvey et al .…”

Section: Discussionmentioning

confidence: 99%

“…In addition, higher ferritin concentrations and ferritin‐binding activities of antibodies in colostrum were found, suggesting that colostral ferritin and autoantibodies to ferritin were transferred to newborn calves (Kanno et al . ).…”

Section: Introductionmentioning

confidence: 97%

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Change of antibody levels to ferritin in the sera of foals after birth: Possible passive transfer of maternal anti‐ferritin autoantibody via colostrum and age‐related anti‐ferritin autoantibody production

Numata

Kondo

Nambo

et al. 2013

Animal Science Journal

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Antibody (immunoglobulin G (IgG), IgM or IgA) levels relative to ferritin in six foal sera (three male and three female) after birth (day 0 and 2, 6, 10, 20, 28, 36, 40, 52 and 56 weeks of age) were semi-quantitatively measured with normalization with antibody activity to ferritin in one adult horse serum. After addition of horse spleen ferritin to the serum sample, the complex formed between antibodies to ferritin in the serum and ferritin was co-immunoprecipitated using antibody to horse spleen ferritin. Antibody classes of the co-immnoprecipitate were detected with antibodies specific for horse IgG, IgM or IgA heavy chain. Six adult horse serum samples were found to have ferritin-binding activities in all immunoglobulin classes examined. Although ferritin antibody activities (IgG, IgM and IgA) were scant in the foal sera before sucking colostrum (day 0), their activities increased at 2 weeks of age. IgG antibodies showed a biphasic response and IgM antibody activity increased up to 40 weeks of age. Antibody (IgG, IgM and IgA) activities to ferritin in three colostrum samples were significantly higher than in adult horse serum samples. These results demonstrate that antibody to ferritin in foal serum is derived from colostrum after birth and is produced thereafter.

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Physiological implications of mammalian ferritin-binding proteins interacting with circulating ferritin and a new aspect of ferritin- and zinc-binding proteins

Orino

2015

Biometals

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Serum ferritin levels are relatively low (<1 µg/ml) and serum ferritin generally disappears rapidly from the circulation (t 1/2 < 10 min). There are various mammalian ferritin-binding proteins (FBPs) in the blood. Ferritin is cleared by direct uptake by ferritin receptors and by indirect receptor-mediated uptake of FBP complexed with ferritin. Mammalian ferritin binds both heme and iron, and binding occurs through two mechanisms: direct binding with ferritin to H-kininogen and anti-ferritin autoantibody, and indirect heme-mediated binding of fibrinogen and apolipoprotein B to ferritin. Anti-ferritin autoantibody and fibrinogen are proposed to be common mammalian FBPs, as is α2-macroglobulin. FBP-ferritin binding may affect blood coagulation and influence iron metabolism, oxidative condition, angiogenesis, inflammatory condition and immune response. Aside from apolipoprotein B, FBPs bind zinc ion to form antioxidant and anti-inflammatory agents. The possible simultaneous uptake of zinc ion with FBP-ferritin complex is likely to attenuate iron- and/or heme-mediated oxidative damage and inflammatory response.

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Measurement of ferritin and anti‐ferritin autoantibodies in serum and colostrum of Holstein and Japanese Black cows

Cited by 3 publications

References 35 publications

RNA-Sequencing for profiling goat milk transcriptome in colostrum and mature milk

RNA-Sequencing for profiling goat milk transcriptome in colostrum and mature milk

Change of antibody levels to ferritin in the sera of foals after birth: Possible passive transfer of maternal anti‐ferritin autoantibody via colostrum and age‐related anti‐ferritin autoantibody production

Physiological implications of mammalian ferritin-binding proteins interacting with circulating ferritin and a new aspect of ferritin- and zinc-binding proteins

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