Measurement of eicosanoids in menstrual fluid by the combined use of high pressure chromatography and radioimmunoassay

Hofer, Gaby; Bieglmayer, Ch.; Kopp, Brigitte; Janisch, H

doi:10.1016/0090-6980(93)90118-q

Cited by 31 publications

(16 citation statements)

References 14 publications

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“…Multiple methods have been developed for the detection and quantitation of prostanoid metabolites. Radio-immuno assays [24], enzyme linked immunoassays [25], HPLC methods [26-29], and gas chromatography-mass spectrometry methods [30, 31] have been developed in the past for the quantitative analysis of these metabolites from many different matrices. Though these assays are useful, limitations of these methods include inadequate sensitivity, limited selectivity, narrow range of metabolites, and cross-reactivity.…”

Section: Introductionmentioning

confidence: 99%

Rapid and simultaneous quantitation of prostanoids by UPLC–MS/MS in rat brain

Shaik

Miller

Graham

et al. 2014

Journal of Chromatography B

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The metabolites of AA produced from the cyclooxygenase (COX) pathway, collectively termed as prostanoids, and from the CYP 450 pathway, eicosanoids, have been implicated in various neurodegenerative and neuroinflammatory diseases. This study developed a quantitative UPLC-MS/MS method to simultaneously measure 11 prostanoids including prostaglandins and cyclopentenone metabolites in the rat brain cortical tissue. Linear calibration curves ranging from 0.104 to 33.3 ng/ml were validated. The inter-day and intra-day variance for all metabolites was less than 15%. The extraction recovery efficiency and matrix (deionized water) effects measured at 12.5 ng/ml (750 pg on column) ranged from 88 to 100% and 3 to 14%, respectively, with % CV values below 20%. Additionally, applying the processing and extraction conditions of this method to our previous CYP450 eicosanoids method resulted in overall improvement in extraction recovery and reduction in matrix effects at low (0.417 ng/ml) and high (8.33 ng/ml) concentrations. In rat brain cortical tissue samples, concentrations of prostanoids ranged from 10.2 to 937 pmol/g wet tissue and concentration of eicosanoids ranged from 2.23 to 793 pmol/g wet tissue. These data demonstrate that the successive measurement of prostanoids and eicosanoids from a single extracted sample of rat brain tissue can be achieved with a UPLC-MS/MS system and that this method is necessary for evaluation of these metabolites as potential biomarkers in delineating their role in various neuroinflammatory and cerebrovascular disorders.

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Section: Introductionmentioning

confidence: 99%

Rapid and simultaneous quantitation of prostanoids by UPLC–MS/MS in rat brain

Shaik

Miller

Graham

et al. 2014

Journal of Chromatography B

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“…Resolution mediators have the potential to regulate these events and promote the transition to endometrial repair. LOX metabolites are among the most abundant AA derivatives present in menstrual blood and include 15-HETE (Hofer et al 1993). Importantly, ANXA1 secretion is detected from cultured human endometrial samples and can be inhibited by progesterone (Gurpide et al 1986).…”

Section: Menstrual Functionmentioning

confidence: 99%

Molecular regulators of resolution of inflammation: potential therapeutic targets in the reproductive system

Hutchinson

Rajagopal²,

Sales³

et al. 2011

Reproduction

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Inflammatory processes are central to reproductive events including ovulation, menstruation, implantation and labour, while inflammatory dysregulation is a feature of numerous reproductive pathologies. In recent years, there has been much research into the endogenous mechanisms by which inflammatory reactions are terminated and tissue homoeostasis is restored, a process termed resolution. The identification and characterisation of naturally occurring pro-resolution mediators including lipoxins and annexin A1 has prompted a shift in the field of anti-inflammation whereby resolution is now observed as an active process, triggered as part of a normal inflammatory response. This review will address the process of resolution, discuss available evidence for expression of pro-resolution factors in the reproductive tract and explore possible roles for resolution in physiological reproductive processes and associated pathologies. Reproduction (2011) 142 15-28

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“…MenstrualJuid was collected on tampons by 12 women who did not use an oral contraceptive but suflered j o m slight primary dysmenorrhea and by three pain-fre women who used an oral contraceptive . Eicosanoids (cycloox ygenase products : 6-ketoprostaglandin Fla, thromboxane Bz, prostaglandin E2, prostaglandin Fza,13,, leukotriene B4, leukotriene C4, leukotriene D4, leukotriene €4) andfemale sex steroids ( I '-/P-estradiol and progesterone) were analyzed by the combined use ofhigh-peformance liquid chromatography and radioimmunoassay. 12-HETE was the main arachidonic acid metabolite.…”

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confidence: 99%

Concentrations of various arachidonic acid metabolites in menstrual fluid are associated with menstrual pain and are influenced by hormonal contraceptives

Bieglmayer

Hofer

Kainz

et al. 1995

Gynecological Endocrinology

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In a pilot study we investigated the association between concentrations of various eicosanoids in menstrual blood with pain and oral contraceptive use. Menstrual fluid was collected on tampons by 12 women who did not use an oral contraceptive but suffered from slight primary dysmenorrhea and by three pain-free women who used an oral contraceptive. Eicosanoids (cyclooxygenase products: 6-ketoprostaglandin F1 alpha, thromboxane B2, prostaglandin E2, prostaglandin F2 alpha, 13,14-dihydro-15-ketoprostaglandin F2 alpha, 12-hydroxy-heptadecatrienoic acid; lipoxygenase products: 5-, 12-, 15-hydroxy-eicosatetraenoic acid (HETE), leukotriene B4, leukotriene C4, leukotriene D4, leukotriene E4) and female sex steroids (17 beta-estradiol and progesterone) were analyzed by the combined use of high-performance liquid chromatography and radioimmunoassay. 12-HETE was the main arachidonic acid metabolite. An increased metabolism of arachidonic acid was associated with pain, especially when synthesis of 12-HETE was elevated. Oral contraceptive use decreased the synthesis of prostaglandins as well as leukotrienes. The concordant changes of cyclooxygenase and lipoxygenase products in dysmenorrhea or in oral contraceptive use may be explained by an increased or decreased phospholipid metabolism, respectively.

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Measurement of eicosanoids in menstrual fluid by the combined use of high pressure chromatography and radioimmunoassay

Cited by 31 publications

References 14 publications

Rapid and simultaneous quantitation of prostanoids by UPLC–MS/MS in rat brain

Rapid and simultaneous quantitation of prostanoids by UPLC–MS/MS in rat brain

Molecular regulators of resolution of inflammation: potential therapeutic targets in the reproductive system

Concentrations of various arachidonic acid metabolites in menstrual fluid are associated with menstrual pain and are influenced by hormonal contraceptives

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