Measurement of Dielectric Properties of Biological Materials Using Co-Axial Fork-Type Probe

Urvashi, .; Zeeshan, Zeeshan; Daya, K. S.; Doneria, Priya

doi:10.1109/jsen.2019.2928117

“…In our earlier work, we reported the denaturing effect of papain on egg proteins, plant protein, and insulin. We found that the dielectric constant decreases on adding papain to proteins 23 . Here, we have extended our investigation to understand the effect of temperature variation in samples of insulin mixed with different concentrations of papain.…”

Section: Resultsmentioning

confidence: 76%

“…To make heating effectively, the isolated box was used for thermal insulation and experimental setup is discussed in supplementary file (See Supplementary File 2 ). The method of calculating the dielectric constant is also previously reported 23 .…”

Section: Methodsmentioning

confidence: 99%

Role of structural specificity of ZnO particles in preserving functionality of proteins in their corona

Singh

¹

,

Saifi

²

,

Kumar

³

et al. 2021

Sci Rep

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Reconfiguration of protein conformation in a micro and nano particle (MNP) protein corona due to interaction is an often-overlooked aspect in drug design and nano-medicine. Mostly, MNP-Protein corona studies focus on the toxicity of nano particles (NPs) in a biological environment to analyze biocompatibility. However, preserving functional specificity of proteins in an NP corona becomes critical for effective translation of nano-medicine. This paper investigates the non-classical interaction between insulin and ZnO MNPs using a classical electrical characterization technique at GHz frequency with an objective to understand the effect of the micro particle (MP) and nanoparticle (NP) morphology on the electrical characteristics of the MNP-Protein corona and therefore the conformation and functional specificity of protein. The MNP-Protein corona was subjected to thermal and enzymatic (papain) perturbation to study the denaturation of the protein. Experimental results demonstrate that the morphology of ZnO particles plays an important role in preserving the electrical characteristics of insulin.

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“…For the present studies, we put the sample solution into polypropylene made sample holder (250 µL Holding capacity) then heat it using hotplate for the range 30 o C to 55 o C. To make heating effectively, the isolated box was used for thermal insulation and experimental setup is discussed in supplementary file (See supplementary file 2). The method of calculating the dielectric constant is also previously reported 23 .…”

Section: Methodsmentioning

confidence: 99%

Role of Structural Specificity of ZnO Particles in Preserving Functionality of Proteins in their Corona

Singh

¹

,

Saifi

²

,

Kumar

³

et al. 2021

Preprint

0

View full text Add to dashboard Cite

Reconfiguration of protein conformation in a micro and nano particle (MNP) protein corona due to interaction is an often-overlooked aspect in drug design and nano-medicine. Mostly, MNP-Protein corona studies focus on the toxicity of nano particles (NPs) in a biological environment to analyze biocompatibility. However, preserving functional specificity of proteins in an NP corona becomes critical for effective translation of nano-medicine. This paper investigates the non-classical interaction between insulin and ZnO MNPs using a classical electrical characterization technique at GHz frequency with an objective to understand the effect of the micro particle (MP) and nanoparticle (NP) morphology on the electrical characteristics of the MNP-Protein corona and therefore the conformation and functional specificity of protein. The MNP-Protein corona was subjected to thermal and enzymatic (papain) perturbation to study the denaturation of the protein. Experimental results demonstrate that the morphology of ZnO particles plays an important role in preserving the electrical characteristics of insulin.

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“…An application to low-moisture rosemary can be found in [ 20 ]. Nevertheless, the extraction of EO from aromatic herbs is an industrial process that can be conducted with the aid of microwave energy as an alternative to conventional hydro-distillation [ 21 ], and permittivity measurements are necessary for equipment design.…”

Section: Introductionmentioning

confidence: 99%

“…The frequency range is, generally, one of the main aspects regarding dielectric measurements of materials. The different studies mentioned range from an extremely low frequency (<1 kHz) [ 17 ], going by 1–1000 Hz [ 10 ], 1.4 GHz [ 19 ], 2.45 GHz [ 20 ], 3.5 GHz [ 14 , 15 ], 5.5–7.5 GHz [ 21 ], 7.5–8.5 GHz [ 15 ], 1–10 GHz [ 3 ], 300 MHz–11 GHz [ 17 ], 300 MHz–18 GHz [ 12 , 13 ], 0.5–40 GHz [ 18 ], and up to 100 GHz [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Permittivity Measurements for Cypress and Rockrose Biomass Versus Temperature, Density, and Moisture Content

Pérez-Campos

¹

,

Fayos-Fernández

²

,

Guerrero

³

et al. 2020

Sensors

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Permittivity of materials is of utmost importance for microwave applicators’ design and to predict high-frequency dielectric heating of materials. In the case of aromatic plant biomass, however, there are few data that help researchers design microwave applicators for microwave-assisted extraction. In this work, the permittivity of cypress and rockrose biomass samples were measured versus temperature, density, and moisture content. A resonant technique based on a coaxial bi-reentrant microwave cavity was employed to obtain the complex permittivity of biomass samples as a function of those magnitudes around the 2.45 GHz ISM frequency. The obtained measurements show that large variations for permittivity values can be found with moisture content and density changes for both cypress and rockrose biomass. Temperature also has effects in a lesser degree, although it has an important influence on the cypress biomass loss factor. Polynomial expressions fitting the experimental data were provided in order to facilitate the estimation of intermediate values, which were not explicitly arranged in this work. As a general trend, the permittivity of cypress and rockrose biomass increases with increasing values of moisture content and density, whereas the biomass loss factor increases when temperature rises.

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Measurement of Dielectric Properties of Biological Materials Using Co-Axial Fork-Type Probe

Cited by 9 publications

References 37 publications

Role of structural specificity of ZnO particles in preserving functionality of proteins in their corona

Role of structural specificity of ZnO particles in preserving functionality of proteins in their corona

Role of Structural Specificity of ZnO Particles in Preserving Functionality of Proteins in their Corona

Permittivity Measurements for Cypress and Rockrose Biomass Versus Temperature, Density, and Moisture Content

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