Measurement of cytotoxic T lymphocyte activity of human cytomegalovirus seropositive individuals by a highly sensitive coupled luminescent method

Ogbomo, Henry; Geiler, Janina; Leutz, Anke; Kietzell, Kristina von; Michaelis, Martin; Činátl, Jindřich

doi:10.1007/s00430-009-0126-5

Cited by 3 publications

(1 citation statement)

References 35 publications

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“…Cytotoxicity assay and Xow cytoXuorometric analysis Cytotoxicity of NK cells was determined by a 4-h coupled luminescent method using the "aCella-Tox" kit (Cell Technology, Mountain View, CA), as previously described [21]. LNCaP and DU145 cell lines, both mock-and NS1 IAVinfected 20-h p.i., were used as target cells.…”

Section: Cell Viability Assaymentioning

confidence: 99%

Tumor cells infected with oncolytic influenza A virus prime natural killer cells for lysis of resistant tumor cells

Ogbomo

Michaelis

Geiler

et al. 2009

Med Microbiol Immunol

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Tumor resistance to lysis by resting natural killer (NK) cells may be overcome by priming of NK cells with cytokines or by binding of NK activating receptors to ligands expressed on target cells. In this study, major histocompatibility complex class I (MHC-I)-negative LNCaP and MHC-I-positive DU145 cells were infected with genetically modified influenza A virus lacking the non-structural gene 1 (NS1 IAV). The cells were used to investigate the influence of NS1 IAV infection on NK cell lysis of tumor cells as well as to prime NK cells for lysis of LNCaP and DU145 cells. While LNCaP cells infected with DeltaNS1 IAV showed enhanced lysis when compared with mock-infected cells (93% +/- 1.47 vs. 52% +/- 0.74), both mock-infected and DeltaNS1 IAV-infected DU145 cells were resistant to NK cell lysis. Moreover, NK cells primed with DeltaNS1 IAV-infected LNCaP/DU145 cells effectively lysed resistant DU145 and sensitive LNCaP cells to a greater extent than NK cells primed with mock-infected LNCaP/DU145 or non-primed NK cells. Also, NK cell priming with DeltaNS1 IAV-infected tumor cells enhanced extracellular signal-regulated kinase phosphorylation and increased granule release in NK cells. The increased granule release was specifically mediated by NKp46, which eventually potentiated NK cells primed with DeltaNS1 IAV-infected tumor cells to overcome the inhibitory effects posed by MHC-I expression on DU145 cells. These findings show that in addition to direct lytic activity of NK cells, DeltaNS1 IAV may influence anti-tumoral responses by priming NK cells.

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Section: Cell Viability Assaymentioning

confidence: 99%

Tumor cells infected with oncolytic influenza A virus prime natural killer cells for lysis of resistant tumor cells

Ogbomo

Michaelis

Geiler

et al. 2009

Med Microbiol Immunol

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Recent publications in medical microbiology and immunology: a retrospective

Doerr

Cinatl

2011

Med Microbiol Immunol

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A look back is done to some clinical and basic research activities recently published in medical microbiology and immunology. The review covers clinical experiences and in vitro experiments to understand the emergency, pathogenicity, epidemic spread, and vaccine-based prevention of avian and swine-origin flu. Some new developments and concepts in diagnosis, (molecular) epidemiology, and therapy of AIDS, viral hepatitis C, and herpesvirus-associated diseases are outlined. Regulation of immune system has been discussed in a special issue 2010 including some aspects of CNS affections (measles). Mycobacterial infection and its prevention by modern recombinant vaccines have reached new interest, as well as new concepts of vaccination and prophylaxis against several other bacteria. Adaptation to host niches enables immune escape (example brucella) and determines virulence (example N. meningitidis). Chlamydia pneumoniae, previously considered to trigger atherosclerosis, is hypothetically associated to Alzheimer disease, while CMV, another putative trigger of atherosclerosis, gains evidence of oncomodulation in CNS tumor diseases. In terms of globalization, exotic virus infections are increasingly imported from southern countries.

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When and How to Evaluate the Natural Killer Cell Function

Queirós

2014

ARRB

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Measurement of cytotoxic T lymphocyte activity of human cytomegalovirus seropositive individuals by a highly sensitive coupled luminescent method

Cited by 3 publications

References 35 publications

Tumor cells infected with oncolytic influenza A virus prime natural killer cells for lysis of resistant tumor cells

Tumor cells infected with oncolytic influenza A virus prime natural killer cells for lysis of resistant tumor cells

Recent publications in medical microbiology and immunology: a retrospective

When and How to Evaluate the Natural Killer Cell Function

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