Measurement of cytogenetic endpoints in women environmentally exposed to air pollution

Michalska, Jadwiga; Motykiewicz, Grażyna; Pendzich, Joanna; Kalinowska, Ewa; Midro, Alina T.; Chorazy, M

doi:10.1016/s1383-5718(99)00120-5

Cited by 15 publications

(8 citation statements)

References 19 publications

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“…CAs are detected as structural chromatid or chromosome-type aberrations, such as breaks and gaps within a chromosome [Carrano and Natarajan, 1988]. In previous studies, MN induction and CAs have been extensively used as biomarkers of genotoxicity [Fenech, 1993;Michalska et al, 1999] and for quantifying adverse human health effects, particularly cancer [Hagmar et al, 1994;Tucker and Preston, 1996]. Our present study indicates that rural Indian women using biofuels for cooking have higher levels of DNA damage than women using cleaner-burning petroleum-based fuels.…”

Section: Introductionmentioning

confidence: 57%

“…Also, a number of biomonitoring studies indicate that occupational or environmental exposure to high levels of PAHs, air pollution, and/or atmospheric PM results in DNA damage [Bender et al, 1988;Bolognesi et al, 1997a,b;Michalska et al, 1999]. In a study that measured the genotoxicity specifically associated with the combustion of biomass fuels, Bell and Kamens [1990] reported that extracts of particles generated from the burning of cowdung, crop residues and wood were mutagenic in the Salmonella typhimurium reversion assay.…”

Section: Discussionmentioning

confidence: 99%

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Cytogenetic biomonitoring of Indian women cooking with biofuels: Micronucleus and chromosomal aberration tests in peripheral blood lymphocytes

Musthapa

Lohani

Tiwari

et al. 2004

Environ and Mol Mutagen

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India currently has the largest number of indoor air pollution-related health problems in the world, with three-quarters of its households burning wood, cowdung, or crop residues ("traditional" biomass fuels) for cooking, and the remainder using kerosene and relatively clean-burning liquefied petroleum gas (LPG). Combustion of these fuels produces various pollutants that may cause serious health effects in exposed populations. In this study, the micronucleus (MN) and chromosomal aberration (CA) assays were used to evaluate the relative amounts of DNA damage produced by the use of these cooking fuels. Cytogenetic evaluation of 179 female subjects showed a significant increase in both MN and CA frequency in blood lymphocytes from users of biomass-fuels in comparison to lymphocytes from LPG users (used as a reference population). The relative MN and CA frequencies for the users of the various fuels decreased in the order cowdung > cowdung/wood >/= wood > kerosene >/= LPG. Further, the results indicated an effect of subject age, and the duration of exposure on the MN and CA frequencies in biomass fuel users. Age had no significant effects on the genotoxicity responses in subjects with 10 years, CA and MN frequencies were higher in older individuals (>30 years of age) than younger subjects. Regardless of age, subjects burning biomass fuels had higher MN and CA frequencies than LPG users only when exposures were of at least 5 years duration. These results indicate that burning biomass-based fuels increases the frequency of cytogenetic alterations in blood lymphocytes of exposed populations, possibly because of exposure to the various noxious gases and toxic substances present in biomass fuels. These cytogenetic markers could be used in the field to assess the genotoxic consequences of burning various cooking fuels and for early detection of genetic abnormalities in people exposed to various pollutants and toxicants.

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Section: Introductionmentioning

confidence: 57%

Section: Discussionmentioning

confidence: 99%

Cytogenetic biomonitoring of Indian women cooking with biofuels: Micronucleus and chromosomal aberration tests in peripheral blood lymphocytes

Musthapa

Lohani

Tiwari

et al. 2004

Environ and Mol Mutagen

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“…This is the first study to report an association between chromosomal aberrations detected by FISH in cord blood and prenatal exposures to airborne PAHs. Similarly, several studies carried out in adult populations have showed a positive relationship between air pollution or PAHs and chromosomal aberrations (26)(27)(28)33). Prior research has indicated an association between PAH-DNA adducts and somatic gene mutation in newborns (40), and between PAH exposure and adducts and chromosomal aberrations in adults (28,33).…”

Section: Discussionmentioning

confidence: 96%

“…Multiple studies comparing genetic damage in persons living in polluted, industrialized urban areas with those living in less polluted, rural areas have found significantly increased levels of carcinogen-DNA and -protein adducts, sister chromatid exchanges, somatic mutation frequencies, and/or chromosomal aberrations in adults (13,(25)(26)(27)(28).…”

Section: Introductionmentioning

confidence: 99%

Chromosomal Aberrations in Cord Blood Are Associated with Prenatal Exposure to Carcinogenic Polycyclic Aromatic Hydrocarbons

Bocskay¹,

Tang²,

Orjuela³

et al. 2005

Cancer Epidemiology, Biomarkers &Amp; Prevention

108

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Molecular and traditional epidemiology studies have indicated a possible relationship between in utero environmental exposures and increased risk for childhood cancers, especially acute leukemias. Chromosomal aberrations have been associated with environmental exposures and cancer risk in adults. In order to more clearly define the association between prenatal exposures to carcinogenic polycyclic aromatic hydrocarbons (PAH) and chromosomal aberrations, chromosomal aberration frequencies were measured in a subset of 60 newborns from the Columbia Center for Children's Environmental Health (CCCEH) Prospective Cohort Study. The subset was composed of African American and Dominican, nonsmoking mother-newborn pairs residing in low-income neighborhoods of New York City, who were exposed to varying levels of airborne PAHs. Prenatal exposure was assessed by questionnaire, personal air monitoring during the third trimester, and PAH-DNA adducts in umbilical cord blood. Chromosomal aberrations were measured in cord blood lymphocytes by fluorescence in situ hybridization. PAH-DNA adducts were not associated with chromosomal aberrations. However, airborne PAHs were significantly associated with stable aberration frequencies in cord blood (P < 0.01). Moreover, stable aberration frequencies were significantly higher among African American newborns compared with Dominican, despite no significant differences in PAH exposure. These results show for the first time an association between prenatal exposure to airborne carcinogenic PAHs and chromosomal aberrations in cord blood, suggesting that such prenatal exposures have the potential to cause cytogenetic damage that has been related to increased cancer risk in other populations.

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“…They have been used widely as a biomarker of cancer risk in adults [Brogger et al, 1990;Chorazy, 1996;Motykiewicz et al, 1998;Huttner et al, 1999;Michalska et al, 1999], and to measure of the effect of environmental pollutants in infants and children [Ramsey et al, 1995;Sram et al, 1999;Pluth et al, 2000;Rossner et al, 2002;Neri et al, 2006a,b].…”

Section: Introductionmentioning

confidence: 99%

Fluorescence in situ hybridization is necessary to detect an association between chromosome aberrations and polycyclic aromatic hydrocarbon exposure in utero and reveals nonrandom chromosome involvement

Bocskay

Orjuela

Tang

et al. 2007

Environ and Mol Mutagen

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Chromosome aberrations are associated with environmental exposures in infants and children. Recently we reported that prenatal exposure to airborne polycyclic aromatic hydrocarbons (PAHs) was significantly (P < 0.01) associated with stable aberration frequencies in cord blood from a subset of 60 newborns from the Columbia Center for Children's Environmental Health Prospective Cohort Study (Bocskay K et al. [2005]: Cancer Epidemiol Biomarkers Prev 14:506-511). To determine whether the environmental exposures may be targeting specific chromosomes and to compare various methods for measuring chromosome aberrations, we further evaluated this same subset of subjects composed of African-American and Dominican nonsmoking mother-newborn pairs residing in low-income neighborhoods of New York City, and exposed to varying levels of airborne PAHs. Chromosome aberrations were measured in cord blood lymphocytes, both by whole chromosome probe (WCP) fluorescence in situ hybridization (FISH) and traditional Giemsa-staining. Prenatal exposures were assessed by personal air monitoring. Breaks in chromosomes 1-6, as detected by WCP FISH, were nonrandomly distributed, underscoring the importance of appropriate chromosome probe selection to capture cytogenetic damage in response to exposure. FISH for stable aberrations was found to be a more sensitive method for detecting aberration frequencies associated with environmental exposures, when compared with FISH for unstable aberrations or Giemsa-staining for aberrations. Together, these results suggest that PAHs may be targeting specific chromosomes and highlight the importance of using the more sensitive detection methods to assess risk in populations with low levels of exposure.

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Measurement of cytogenetic endpoints in women environmentally exposed to air pollution

Cited by 15 publications

References 19 publications

Cytogenetic biomonitoring of Indian women cooking with biofuels: Micronucleus and chromosomal aberration tests in peripheral blood lymphocytes

Cytogenetic biomonitoring of Indian women cooking with biofuels: Micronucleus and chromosomal aberration tests in peripheral blood lymphocytes

Chromosomal Aberrations in Cord Blood Are Associated with Prenatal Exposure to Carcinogenic Polycyclic Aromatic Hydrocarbons

Fluorescence in situ hybridization is necessary to detect an association between chromosome aberrations and polycyclic aromatic hydrocarbon exposure in utero and reveals nonrandom chromosome involvement

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