Measurement of cortisol, cortisone and 11β-hydroxysteroid dehydrogenase type 2 activity in hair of sows during different phases of the reproductive cycle

López-Arjona, Marina; Tecles, Fernando; Mateo, Sandra V.; Contreras-Aguilar, María Dolores; Martínez-Miró, Silvia; Cerón, José J.; Martı́nez-Subiela, Silvia

doi:10.1016/j.tvjl.2020.105458

Cited by 13 publications

(30 citation statements)

References 34 publications

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“…In the first stage, to determine which solvent would be better for the extraction of oxytocin from hair, two different chemicals were tested. One was methanol, which has been demonstrated to be efficient for cortisol and cortisone extraction [7][8][9][10][11], and the second was acetonitrile, which has been used previously for oxytocin extraction from urine [12] or saliva [3].…”

Section: Selection Of the Solvent For The Sample Extraction Proceduresmentioning

confidence: 99%

“…In both cases, the procedure for hair processing was the same, and it was based on a previous procedure used for cortisol [13] and cortisone [7] extraction. The hair samples from the sows were weighed (250 mg), washed with isopropanol twice, and left at room temperature (RT) until completely dry.…”

Section: Selection Of the Solvent For The Sample Extraction Proceduresmentioning

confidence: 99%

“…Stored samples from a total of 20 multiparous Large White sows, which were used in a previous research to measure cortisol and cortisone in hair of pigs, were included in this study [7].…”

Section: Study Animals and Samplingmentioning

confidence: 99%

“…For oxytocin measurement in hair samples, the assay developed in this study was used. For the cortisol and cortisone determination, an indirect competitive alphaLISA was used in the previous study [7]. For cortisol determination, a commercially available monoclonal antibody against cortisol obtained from a mouse was used, while for cortisone determination, a polyclonal antibody against cortisone was used.…”

Section: Study Animals and Samplingmentioning

confidence: 99%

“…Hair has been used as a non-invasive way to evaluate the stress response in animals through the measurement of cortisol [6] and cortisone [7]. In addition to the advantage of being a non-invasive sample, in some cases, such as cortisol, hair is useful in the evaluation of the long-term evolution of the dynamics of this analyte in the body [7][8][9][10]. In pigs, previous reports indicated that hair samples can provide information to a period varying from one to two months before the date of collection [8].…”

Section: Introductionmentioning

confidence: 99%

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A Procedure for Oxytocin Measurement in Hair of Pig: Analytical Validation and a Pilot Application

López-Arjona

Tecles

Mateo

et al. 2021

Biology

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There is growing interest in oxytocin as a biomarker of stress and welfare. The objective of this study was to develop and validate a procedure based on a highly sensitive immunoassay to measure oxytocin in the hair of pigs. In addition, a pilot study to apply this procedure to evaluate possible changes in concentrations of oxytocin in hair during the reproductive cycle of pigs at different periods of the year was conducted. This procedure used methanol for sample extraction, since it offered better recoveries than acetonitrile, and the immunoassay developed was precise and accurate for the quantification of the oxytocin in the hair. When this procedure was applied to hair collected at different times of the reproductive cycle and season, higher values were found at days 23 and 59 after farrowing in the winter–spring period. In addition, higher oxytocin values in the spring–summer period were found in hair collected 5 days before farrowing compared to winter–spring. Oxytocin in hair showed moderate and low correlations with cortisone and cortisol in hair, respectively. This study represents the first report in which oxytocin was measured in hair and could open new lines for future research about the measurement of oxytocin in pigs and other biological species as a biomarker of stress.

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Section: Selection Of the Solvent For The Sample Extraction Proceduresmentioning

confidence: 99%

Section: Selection Of the Solvent For The Sample Extraction Proceduresmentioning

confidence: 99%

“…Stored samples from a total of 20 multiparous Large White sows, which were used in a previous research to measure cortisol and cortisone in hair of pigs, were included in this study [7].…”

Section: Study Animals and Samplingmentioning

confidence: 99%

Section: Study Animals and Samplingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

A Procedure for Oxytocin Measurement in Hair of Pig: Analytical Validation and a Pilot Application

López-Arjona

Tecles

Mateo

et al. 2021

Biology

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Determination of hair cortisol in horses: comparison of immunoassay vs LC-HRMS/MS

et al. 2022

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The measure of hair cortisol concentration (HCC) is becoming an emerging approach to monitor mid-/long-term stress in animals, so it is more and more important to develop accurate and reliable methods. In the light of this, the aim of the present study was to compare mane HCCs of 47 horses with different managements, by means of an immunoassay (ELISA) and liquid chromatography coupled to hybrid high-resolution mass spectrometry (LC-HRMS/MS). After the washing step, the ground hair was extracted with methanol. The extract was evaporated and redissolved in two different aqueous solutions, depending on the detection technique. The methods were validated according to EMA guideline for bioanalytical method validation, in the range 2–50 pg mg−1 (ELISA) and 1–100 pg mg−1 (LC-HRMS/MS). Satisfactory quantitative performances were obtained for both of the approaches, but this latter demonstrated better precision. The detected concentrations in real samples were encompassing the range 1.3–8.8 pg mg−1 and 2.0–17.9 pg mg−1 by means of LC-HRMS/MS and ELISA, respectively. Overall, HCCs measured with ELISA technique were 1.6 times higher. The overestimation of immunoassay results might be caused by cross-reactivity phenomena of laboratory reagents and other structurally similar hormones present in the mane. Graphical abstract

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Consequences of timing of organic enrichment provision on pig performance, health and stress resilience after weaning and regrouping

Bučková¹,

Muns²,

Cerón³

et al. 2022

animal

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Measurement of cortisol, cortisone and 11β-hydroxysteroid dehydrogenase type 2 activity in hair of sows during different phases of the reproductive cycle

Cited by 13 publications

References 34 publications

A Procedure for Oxytocin Measurement in Hair of Pig: Analytical Validation and a Pilot Application

A Procedure for Oxytocin Measurement in Hair of Pig: Analytical Validation and a Pilot Application

Determination of hair cortisol in horses: comparison of immunoassay vs LC-HRMS/MS

Consequences of timing of organic enrichment provision on pig performance, health and stress resilience after weaning and regrouping

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