Measurement of Corticosterone Metabolites in Birds' Droppings: An Analytical Approach

Möstl, Erich; Rettenbacher, Sophie; Palme, Rupert

doi:10.1196/annals.1343.004

Cited by 223 publications

(233 citation statements)

References 50 publications

Supporting

Mentioning

219

Contrasting

Unclassified

Order By: Relevance

“…There are now several papers reviewing how to interpret faecal hormone (or hormone metabolite) measures with respect to plasma measures (e.g. Möstl and Palme, 2002;Millspaugh and Washburn, 2004;Goymann, 2005;Möstl et al, 2005;Palme et al, 2005;Touma and Palme, 2005). A small number of studies have simultaneously measured plasma hormones and faecal hormone metabolites to detect changes following artificial stimulation and suppression in domestic animals Palme et al, 1999;Dehnhard et al, 2003); however, to our knowledge no studies have specifically quantified the relationship between plasma and faecal measures in a natural context, nor examined the consistency of results obtained when plasma hormone and faecal hormone metabolite measures are used simultaneously to assess baseline hormone concentrations.…”

Section: Introductionmentioning

confidence: 99%

Comparing plasma and faecal measures of steroid hormones in Adelie penguins Pygoscelis adeliae

et al. 2009

View full text Add to dashboard Cite

Abstract. Physiological measurements of both stress and sex hormones are often used to estimate the consequences of natural or human-induced change in ecological studies of various animals. Different methods of hormone measurement exist, potentially explaining variation in results across studies; methods should be cross-validated to ensure they correlate.We directly compared faecal and plasma hormone measurements for the first time in a wild free-living species, the Adelie penguin (Pygoscelis adeliae). Blood and faecal samples were simultaneously collected from individual penguins for comparison, and assayed for testosterone and corticosterone (or their metabolites). Sex differences and variability within each measure, and correlation of values across measures were compared. For both hormones, plasma samples showed greater variation than faecal samples. Males had higher mean corticosterone concentrations than females, but the difference was only statistically significant in faecal samples. Plasma testosterone, but not faecal testosterone, was significantly higher in males than females. Correlation between sample types was poor overall, and weaker in females than in males, perhaps because measures from plasma represent hormones that are both free and bound to globulins, whereas measures from faeces represent only the free portion. Faecal samples also represent a cumulative measure of hormones over time, as opposed to a plasma 'snapshot' concentration. Our data indicate that faecal sampling appears more suitable for assessing baseline hormone concentrations, while plasma sampling may best define immediate responses to environmental events. Consequently, future studies should ensure they select the most appropriate matrix and method of hormone measurement to answer their research questions.

show abstract

Section: Introductionmentioning

confidence: 99%

Comparing plasma and faecal measures of steroid hormones in Adelie penguins Pygoscelis adeliae

et al. 2009

View full text Add to dashboard Cite

show abstract

“…Faeces also have the advantage of enabling time-integrated measures of GC. However, most native GCs are metabolised in a speciesspeciWc manner before excretion, and resulting GC metabo- ( Möstl et al 2005). Therefore, assays for GC metabolites need to be validated for each species.…”

Section: Introductionmentioning

confidence: 99%

Non-invasive measurement of faecal glucocorticoid metabolites in Upland Geese Chloephaga picta

et al. 2008

Self Cite

View full text Add to dashboard Cite

Glucocorticoid (GC) hormones rise in response to stressors, including natural events including weather or predator presence, and human activities, such as hunting, scientiWc research or recreational visits. However, because blood sampling itself causes stress and is dangerous or even impossible in some wildlife species, feedback-free methods for GC determination are needed to assess stress in these animals. Faecal GC analyses have thus gained interest. Here, we validate a non-invasive method to estimate the physiological stress in the Upland goose Chloephaga picta. An adrenocorticotropin hormone (ACTH) challenge was conducted in captive adults (female and male), and droppings were collected before, during and after the experiment. Corticosterone metabolite (CM) secretion in response to the ACTH challenge was measured with several enzyme immunoassays (EIA) to Wnd the most appropriate test. We used CM levels during the periods before and after the experiment as control data. An EIA for 11-oxoetiocholanolone achieved the highest response to the ACTH challenge and also reXected a stress response to unfamiliar environment. Furthermore, CM concentrations of dry samples were highly correlated with the corresponding non-dried (frozen) samples. The data suggest that this method is appropriate to measure the stress in Upland geese, and that samples can be stored either frozen or dry form.

show abstract

“…Em animais selvagens, tais condições são raramente encontradas, pois estes são facilmente estressáveis, o que resulta na alteração dos valores hormonais plasmáticos em poucos segundos, principalmente no que se refere aos hormônios relacionados ao estresse. Adicionalmente, além da elevada probabilidade de ocorrência de traumas no momento da contenção, no caso de animais de porte muito pequeno, o volume de sangue possível de ser colhido pode ser insuficiente e as vias de acesso para a sua colheita restritas 15 . Devido a todos esses fatores, em animais selvagens têm-se preconizado a mensuração hormonal nas excreções, tais como fezes e urina, por sua facilidade de obtenção e maior segurança tanto para o animal quanto para o pesquisador 15 .…”

Section: Discussionunclassified

“…Adicionalmente, além da elevada probabilidade de ocorrência de traumas no momento da contenção, no caso de animais de porte muito pequeno, o volume de sangue possível de ser colhido pode ser insuficiente e as vias de acesso para a sua colheita restritas 15 . Devido a todos esses fatores, em animais selvagens têm-se preconizado a mensuração hormonal nas excreções, tais como fezes e urina, por sua facilidade de obtenção e maior segurança tanto para o animal quanto para o pesquisador 15 . Os esteroides, como a corticosterona e a progesterona, por sofrerem metabolização hepática, não estão presentes nas fezes nas suas formas originais e sim na forma de metabólitos.…”

Section: Discussionunclassified

Dosagem de metabólitos de glucocorticoides e progesterona em fezes de papagaio-verdadeiro (Amazona aestiva)

Fujihara¹,

Filho²,

Monteiro³

et al. 2014

Ciênc. anim. bras.

View full text Add to dashboard Cite

RESUMOOs objetivos do presente trabalho foram avaliar as concentrações fecais de metabólitos de glicocorticoides, mensurados por enzimaimunoensaio, empregando-se anticorpos contra cortisol, e por radioimunoensaio, empregando-se anticorpo contra corticosterona, e dos metabólitos da progesterona, mensurados por raioimunoensaio empregando-se anticorpo contra progesterona, em papagaios-verdadeiros (Amazona aestiva) após desafio com ACTH. A estimulação da adrenal com 25 UI/animal de ACTH resultou na elevação das concentrações de metabólitos de glicocorticoides, mas não modificou a dos metabólitos da progesterona fecal em papagaio-verdadeiro. Embora não tenha sido observada a sincronização dos picos de excreção fecal dos metabólitos de glicocorticoides mensurados por enzimaimunoensaio e radioimunoensaio, houve dois picos de excreção, um entre 2 e 4 horas e outro entre 8 e 10 horas. Apesar dos picos, não foram detectados efeitos de Grupos (Tratamento x Controle), momento (horas de coleta) ou sexo (macho x fêmea) nos resultados observados nas concentrações fecais de metabólitos de glicocorticoides e de progesterona, com os métodos empregados. PALAVRAS-CHAVE: aves, corticosterona, cortisol, não-invasivo, progesterona. MEASUREMENT OF GLUCOCORTICOID AND PROGESTERONE METABOLITES IN FECES OF BLUE FRONTED PARROT (Amazona aestiva) ABSTRACTThe objectives of the present study were to evaluate fecal concentrations of metabolites of glucocorticoids, measured by enzyme immunoassay with a cortisol antibody and by radioimmunoassay with a corticosterone antibody, and progesterone by radioimmunoassay with a progesterone antibody in blue-fronted parrot (Amazona aestiva) after ACTH challenge. The adrenal stimulation with ACTH (25 UI/animal) resulted in an increase of fecal glucocorticoids metabolites concentration, but it did not affect the concentrations of fecal progesterone metabolites. Although there were no synchronized peaks of glucocorticoid metabolites excretion measured by enzyme immunoassay and radioimmunoassay, there were two peaks of excretion, one at 2-4 hours and other at 8-10 hours. Despite the occurrence of peaks, the analysis of fecal glucocorticoids metabolites and progesterone

show abstract

Measurement of Corticosterone Metabolites in Birds' Droppings: An Analytical Approach

Cited by 223 publications

References 50 publications

Comparing plasma and faecal measures of steroid hormones in Adelie penguins Pygoscelis adeliae

Comparing plasma and faecal measures of steroid hormones in Adelie penguins Pygoscelis adeliae

Non-invasive measurement of faecal glucocorticoid metabolites in Upland Geese Chloephaga picta

Dosagem de metabólitos de glucocorticoides e progesterona em fezes de papagaio-verdadeiro (Amazona aestiva)

Contact Info

Product

Resources

About