Measurement of corneal and limbal epithelial thickness by anterior segment optical coherence tomography and in vivo confocal microscopy

Le, Qihua; Chen, Yan; Yang, Yujing; Xu, Jianjiang

doi:10.1186/s12886-016-0342-x

Cited by 23 publications

(10 citation statements)

References 15 publications

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“…Consistent with previous data (3,4,5,13,14,17,25,26), our ndings demonstrated that the corneal epithelium was nonuniform. The superior epithelium was thinner than the inferior one.…”

Section: Discussionsupporting

confidence: 93%

“…Numerous diagnostic modalities are available to quantify corneal thickness (CT) and ET such as very high-frequency (VHF) digital ultrasound, confocal microscopy, and anterior segment optical coherence tomography (AS-OCT) (13,14). AS-OCT is a non-contact, high-resolution imaging technique that allows precise delineation of corneal surfaces (15).…”

Section: Introductionmentioning

confidence: 99%

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Corneal Epithelium Thickness Profile in Normal Adults

Salman,

Kailani,

Omran

et al. 2023

Preprint

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Aim To characterize the corneal epithelial thickness (ET) and corneal thickness (CT) profiles in normal eyes of Syrian adults, and to investigate associated factors with central epithelial thickness (CET). Method ET and CT were measured by Cirrus high-definition optical coherence tomography (HD-OCT) device in 208 eyes of 104 healthy adults aged 18 to 40 years. The average ET and CT were calculated in 25 (9 mm circle) zones and 17 (7 mm circle) zones, respectively. Correlations of CET with age, refractive errors, keratometry, and central corneal thickness (CCT) were calculated. Results The average ET was thicker in the central 2 mm than the paracentral, midperipheral and peripheral zones (50.42 ± 3.51 µm, 49.29 ± 3.61 µm, 47.80 ± 3.54 µm, and 46.74 ± 3.47 µm, respectively), whereas the average CT was thinnest in the centre. While Males have thicker ET than females in all zones (p < 0.05, all), CT did not significantly differ (p > 0.05, all). ET was thinner in the superior area than in the inferior, with the highest difference registered in the peripheral zone (-6 ± 5 µm). We found a positive significant correlation between CET and sphere (r = 0.25, p = 0.0022), and a negative significant correlation between CET and flat keratometry (r = -0.148, p = 0.043). CET was not correlated with age, cylinder or steep keratometry. Conclusion We present a comprehensive study in healthy, normal eyes using Cirrus HD-OCT to map the corneal epithelium with a 9 mm diameter. The epithelium was thinner centrally than peripherally and superiorly than inferiorly. Myopic eyes tend to have thinner corneal epithelium than hyperopic eyes. This may help in refractive procedures and in the prediction of corneal diseases.

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“…Consistent with previous data (3,4,5,13,14,17,25,26), our ndings demonstrated that the corneal epithelium was nonuniform. The superior epithelium was thinner than the inferior one.…”

Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Corneal Epithelium Thickness Profile in Normal Adults

Salman,

Kailani,

Omran

et al. 2023

Preprint

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“…One study defined the limbus as having a 1-mm width extending centripetally from the scleral spur. The mean LETs were 75.23 μm in the superior quadrant, 75.29 μm in the inferior quadrant, 61.18 μm in the nasal quadrant, and 61.02 μm in the temporal quadrant, 17 all of which are in agreement with our measurements made in the Chinese group. However, in another study on a Han population that arbitrarily defined the limbus located 1.5-mm extending centripetally from the scleral spur in every limbal quadrant, the mean LETs in the four quadrants were found to be significantly less than the value observed in the current study.…”

Section: Discussionsupporting

confidence: 90%

In Vivo Evaluation of the Limbus Using Anterior Segment Optical Coherence Tomography

Cordova

et al. 2018

Trans. Vis. Sci. Tech.

Self Cite

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PurposesTo investigate the limbal structure using anterior segment optical coherence tomography (AS-OCT) and compare the difference between a Chinese Han population and a Caucasian population.MethodsSixty healthy Chinese Han subjects (109 eyes, Chinese group) and 32 healthy Caucasian subjects (51 eyes, Caucasian group) were included in this comparative cross-sectional study. The central cornea and the superior, inferior, nasal, and temporal limbal regions of each subject underwent Fourier-domain AS-OCT. The following parameters were measured: corneal epithelial thickness (CET), maximum limbal epithelial thickness (LET), the mean LET, the width of limbus, distance between scleral spur and the location where limbal epithelium was the thickest (S-T), and limbal epithelial area between scleral spur and the end of Bowman's layer (LEA).ResultsCET was similar in both groups (P = 0.577). The width of limbus was more than 32.8% greater in all limbal quadrants in the Caucasian group (range, 1.25–2.20 mm) than in the Chinese group (range, 0.81–1.40 mm). S-T and LEA were also significantly higher in all limbal quadrants in the Caucasian group (all P < 0.001). The maximum LET and mean LET were 7.8% and 6.9% thicker at the nasal limbus and 8.1% and 8.7% thicker in the temporal limbus in Caucasian subjects than in Chinese subjects.ConclusionsThe limbal structures can be visualized using AS-OCT and differ significantly between the Caucasian and Chinese eyes.Translational RelevanceResearch of the limbus and surgeons performing procedures involving the limbal area should take into consideration of the anatomic differences especially when limbus is used as an anatomic reference.

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“…The external limbal location has served as a surrogate for the internal anterior chamber angle. The limbus is an area where the cornea transitions to the sclera [ 1 ]. Surgically, the anterior limbal line can be seen externally as the ridge where the conjunctiva inserts into the corneal margin.…”

Section: Introductionmentioning

confidence: 99%

Displacement between anterior chamber width obtained by swept-source anterior segment optical coherence tomography and white-to-white distance

et al. 2021

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Purpose To determine the relationship between the external limbal location, represented by white-to-white (WTW) distance, and the actual angle location, represented by spur-to-spur (STS) and angle-to-angle (ATA) distances. Methods 166 eyes from 166 participants were imaged using CASIA2 anterior chamber optical coherence tomography (AS-OCT) and LenStar LS 900 optical biometer. The horizontal ATA and STS were measured using the swept-source Fourier-domain AS-OCT (CASIA2). The horizontal WTW was automatically measured using LenStar. The displacement lengths (DL) between WTW-STS and WTW-ATA were calculated. Bland-Altman plots and intraclass correlation were performed. Results The study showed that WTW has a positive correlation with STS (ICC = 0.82, p<0.001) and ATA (ICC = 0.82, p<0.001). The Bland-Altman analysis demonstrated that the mean difference of WTW-STS is 0.10 mm (95% CI 0.06 to 0.14 mm) with limits of agreement of -0.42 to 0.63 mm between WTW and STS, and the mean difference of WTW-ATA is 0.10 mm (95% CI 0.06 to 0.15 mm) with limits of agreement of -0.48 to 0.64 mm between WTW and ATA. Linear regression with adjustment showed that a WTW value greater than 12.07 mm is associated with a greater DL (WTW-STS DL ß 0.18, p = 0.003; WTW-ATA DL ß 0.14, p = 0.03). Conclusions Greater WTW was significantly associated with higher displacement of WTW from the two distances representing anterior chamber width. External limbal location may not accurately represent the actual angle location in eyes with larger WTW.

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Measurement of corneal and limbal epithelial thickness by anterior segment optical coherence tomography and in vivo confocal microscopy

Cited by 23 publications

References 15 publications

Corneal Epithelium Thickness Profile in Normal Adults

Corneal Epithelium Thickness Profile in Normal Adults

In Vivo Evaluation of the Limbus Using Anterior Segment Optical Coherence Tomography

Displacement between anterior chamber width obtained by swept-source anterior segment optical coherence tomography and white-to-white distance

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